Method for extracting spica prunellae and rosmarinus officinalis acid on basis of response surface processes

A technology based on rosmarinic acid and response surface method, which is applied in the field of extracting rosmarinic acid from Prunella vulgaris based on response surface method, can solve the problems of short cycle, high precision, and no consideration of interaction, etc., and achieve the effect of efficient extraction

Inactive Publication Date: 2018-10-16
TONGREN POLYTECHNIC COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional univariate optimization test has the disadvantage of not considering the interaction between various factors, while the response surface method test is a comprehensive technology of the optimization process, which has the characteristics of less test times, short cycle time, and high precision.

Method used

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  • Method for extracting spica prunellae and rosmarinus officinalis acid on basis of response surface processes
  • Method for extracting spica prunellae and rosmarinus officinalis acid on basis of response surface processes
  • Method for extracting spica prunellae and rosmarinus officinalis acid on basis of response surface processes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] drying and crushing Prunella vulgaris into 60 meshes to obtain Prunella vulgaris powder;

[0038] Weigh 20.0000g of Prunella vulgaris powder, add acetic acid-sodium acetate buffer solution, enzymatically hydrolyze for 2 hours under the conditions of enzymolysis temperature 50°C, enzymolysis pH value 4.0, cellulase dosage 16mg / g, and filter the enzymolysis material , to obtain crude extract;

[0039] Concentrate the crude extract to a thick paste with a rotary evaporator, and dry it in a vacuum oven for 24 hours to obtain the crude extract.

[0040] Take above-mentioned crude thing by weighing, adopt anhydrous methanol to dissolve, after dilution, adopt high performance liquid chromatography to measure Prunella vulgaris rosmarinic acid, and according to rosmarinic acid yield Y=[(rosmarinic acid content × extract weight) / Prunella vulgaris medicinal material weight]×100%. The calculated yield of rosmarinic acid was 20.25 mg / g.

Embodiment 2

[0042] drying and crushing Prunella vulgaris into 60 meshes to obtain Prunella vulgaris powder;

[0043] Weigh 20.0000g of Prunella vulgaris powder, add acetic acid-sodium acetate buffer solution, enzymatically hydrolyze for 2 hours under the conditions of enzymolysis temperature 45°C, enzymolysis pH value 4.5, cellulase dosage 12mg / g, and filter the enzymolysis material , to obtain crude extract;

[0044] Concentrate the crude extract to a thick paste with a rotary evaporator, and dry it in a vacuum oven for 24 hours to obtain the crude extract.

[0045] Take above-mentioned crude thing by weighing, adopt anhydrous methanol to dissolve, after dilution, adopt high performance liquid chromatography to measure Prunella vulgaris rosmarinic acid, and according to rosmarinic acid yield Y=[(rosmarinic acid content × extract weight) / Prunella vulgaris medicinal material weight]×100%. The calculated yield of rosmarinic acid was 23.79 mg / g.

Embodiment 3

[0047] drying and crushing Prunella vulgaris into 60 meshes to obtain Prunella vulgaris powder;

[0048] Weigh 20.0000g of Prunella vulgaris powder, add acetic acid-sodium acetate buffer solution, enzymatically hydrolyze for 2 hours under the conditions of enzymolysis temperature 50°C, enzymolysis pH value 5.0, cellulase dosage 16mg / g, and filter the enzymolysis material , to obtain crude extract;

[0049] Concentrate the crude extract to a thick paste with a rotary evaporator, and dry it in a vacuum oven for 24 hours to obtain the crude extract.

[0050] Take above-mentioned crude thing by weighing, adopt anhydrous methanol to dissolve, after dilution, adopt high performance liquid chromatography to measure Prunella vulgaris rosmarinic acid, and according to rosmarinic acid yield Y=[(rosmarinic acid content × extract weight) / Prunella vulgaris medicinal material weight]×100%. The calculated yield of rosmarinic acid was 26.13mg / g.

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Abstract

The invention belongs to the technical field of spica prunellae extraction, and particularly discloses a method for extracting spica prunellae and rosmarinus officinalis acid on the basis of responsesurface processes. The method has the advantages that spica prunellae is extracted by the aid of cellulase enzymatic hydrolysis processes, technological parameters such as the enzymatic hydrolysis temperatures, the enzymatic hydrolysis pH (potential of hydrogen) values, the cellulase usage and the enzymatic hydrolysis time are reasonably determined and selected in extraction procedures, accordingly, the yield of the spica prunellae and rosmarinus officinalis acid can reach 17.69 mg/g at least, toxic organic solvents can be effectively omitted, the purpose of efficiently extracting the spica prunellae and rosmarinus officinalis acid can be achieved, and the method is high in efficiency and is controllable and stable.

Description

technical field [0001] The invention belongs to the technical field of Prunella vulgaris extraction, in particular to a method for extracting Prunella vulgaris rosmarinic acid based on the response surface method. Background technique [0002] Prunella vulgaris is the dried fruit ear of Prunella vulgaris L., a plant of the Labiatae family. Pharmacological studies have shown that the main component of Prunella vulgaris is rosmarinic acid, which has antibacterial, antiviral, and antioxidant activities. About Prunella rosmarinic acid Ultrasonic extraction and reflux extraction have been reported in the literature. Ultrasonic extraction uses ethanol as the extraction solvent, which has the disadvantages of a large amount of extraction solvent and special requirements for extraction equipment. The reflux extraction also uses ethanol as the extraction solvent. Disadvantages such as large amount of organic solvent and long extraction time. [0003] Enzymatic extraction is a bioeng...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/52
CPCC07C67/48C07C67/52C07C69/732
Inventor 韩建军宁娜
Owner TONGREN POLYTECHNIC COLLEGE
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