Rapid establishment and propagation method for ardisia lindleyana plant resources
A Zijinniu and coastal technology, applied in the field of plant propagation, can solve the problems of small quantity, natural reproduction ability, weak propagation and diffusion ability, etc., and achieve the effect of less pests and diseases, good growth and improved rooting rate
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Embodiment 1
[0018] 1) Material selection and disinfection: Cut the plump and mature coastal Zijinniu berries, wrap them in a fresh-keeping bag, and bring them back to the laboratory, remove the impurities around the Zijinniu berries and remove the arils, and put the saturated bleach supernatant Soak in water for 15 minutes, then rinse in running water for 0.5 hours, and gently scrub with a soft brush. After rinsing, rinse twice with double distilled water, put it on an ultra-clean workbench and disinfect it with 75% alcohol for 30 seconds, then pour off the alcohol , add 0.1% liter HgCl 2 Treat for 10min, then HgCl 2 Pour the mercury solution into a waste mercury bottle, rinse it with sterile water for 4 times, dry the surface moisture with sterile filter paper, and set aside;
[0019] 2) Induction culture: Cut the fruit longitudinally with a sterilized scalpel, pick up the powdered seeds with tweezers, move them to the mouth of the culture bottle, tap the tweezers lightly, so that the s...
Embodiment 2
[0026] 1) Material selection and disinfection: Cut the plump and mature coastal Zijinniu berries, wrap them in a fresh-keeping bag, bring them back to the laboratory, remove the impurities around the Zijinniu berries and remove the arils, and put the supernatant of saturated bleaching powder Soak in water for 15 minutes, then rinse in running water for 0.5 hours, and gently scrub with a soft brush. After rinsing, rinse with double distilled water for 3 times, put it on an ultra-clean workbench and disinfect it with 75% alcohol for 30 seconds, then pour off the alcohol , add 0.1% liter HgCl 2 Treat for 13min, then HgCl 2 Pour the mercury solution into a waste mercury bottle, rinse it with sterile water for 6 times, dry the surface moisture with sterile filter paper, and set aside;
[0027] 2) Induction culture: cut the fruit longitudinally with a sterilized scalpel, pick up the powdered seeds with tweezers, move them to the mouth of the culture bottle, tap the tweezers lightly...
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