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Method for preparing water-soluble nano-selenium microsphere based on L.casei 393

A technology of Lactobacillus casei and nano-selenium, which is applied in the field of bioengineering and nano-material preparation, can solve problems such as complex technical process, low bioavailability, and unsafe factors, and achieve simple preparation technology, high bioavailability, water-soluble good sex effect

Pending Publication Date: 2018-10-19
NORTHWESTERN POLYTECHNICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to avoid the deficiencies of the prior art, the present invention proposes a method for preparing water-soluble nano-selenium microspheres based on Lactobacillus casei Lactobacillus casei 393, L.casei 393, to solve the problem of selenium additives in existing food, feed or medicine There are problems such as strong toxicity, poor water solubility, low bioavailability, large environmental pollution, complex production and preparation processes, high production costs, and unsafe factors.

Method used

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  • Method for preparing water-soluble nano-selenium microsphere based on L.casei 393

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Embodiment 1

[0036] Embodiment 1: the preparation method of Lactobacillus casei L.casei 393 nano-selenium microspheres

[0037] A method utilizing Lactobacillus casei L.casei 393 to convert toxic sodium selenite into nontoxic intracellular biological nano-selenium, and separating and extracting to prepare nano-selenium microspheres is implemented according to the following steps:

[0038] Step 1, strain activation:

[0039] Lactobacillus casei L.casei 393 strain was activated and cultured with MRS solid medium, and the formula of MRS solid medium was as follows: peptone 1±0.1%, beef 1±0.1%, yeast extract 0.5±0.01%, diammonium hydrogen citrate 0.2 ±0.01%, glucose 2±0.1%, sodium acetate 0.5±0.01%, dipotassium hydrogen phosphate 0.2±0.01%, magnesium sulfate heptahydrate 0.06±0.01%, manganese sulfate monohydrate 0.025±0.01%, Tween 80 0.1±0.01 %, agar 2±0.1%, pH 6.2-6.6; inoculate L. casei 393 in MRS solid medium by streaking, and culture anaerobically at 37±1℃ for 48±2h.

[0040] Step 2, ana...

Embodiment 2

[0045] Embodiment 2: the preparation method of Lactobacillus casei L.casei 393 nanometer selenium microspheres of different characteristics

[0046] The present invention also provides a method for preparing nano-selenium microspheres with different particle sizes, different selenium, protein and polysaccharide contents by using Lactobacillus casei L.casei 393, which is implemented according to the following steps:

[0047] Step 1, strain activation:

[0048] Lactobacillus casei L.casei 393 strain was activated and cultured with MRS solid medium, and the formula of MRS solid medium was as follows: peptone 1±0.1%, beef 1±0.1%, yeast extract 0.5±0.01%, diammonium hydrogen citrate 0.2 ±0.01%, glucose 2±0.1%, sodium acetate 0.5±0.01%, dipotassium hydrogen phosphate 0.2±0.01%, magnesium sulfate heptahydrate 0.06±0.01%, manganese sulfate monohydrate 0.025±0.01%, Tween 80 0.1±0.01 %, agar 2±0.1%, pH 6.2-6.6; inoculate L. casei 393 in MRS solid medium by streaking, and culture anaero...

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Abstract

The invention relates to a method for preparing water-soluble nano-selenium microspheres based on L.casei 393. L.casei 393 is an abbreviation of Lactobacillus casei 393. The method comprises the following steps: carrying out activation culture on an L.casei 393 strain by utilizing an MRS (DeMan, Rogosa and Sharpe) solid culture medium; further carrying out anaerobic selenium enrichment culture, and converting toxic sodium selenite into nontoxic intracellular biological nano-selenium by utilizing the L.casei 393; carrying out separation extraction on the intracellular nano-selenium of the L.casei 393, thereby obtaining nano-selenium microsphere freeze-dried powder. The particle sizes of the obtained nano-selenium microspheres are 30-300nm, surfaces of the nano-selenium microspheres are coated by polysaccharides and proteins, and in addition, the nano-selenium microspheres are easily dissolved in water. The nano-selenium microspheres are good in anti-oxidation activity.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and nano-material preparation, and relates to a method for preparing water-soluble nano-selenium microspheres based on Lactobacillus casei 393 and L. casei 393. Background technique [0002] Selenium is an essential trace element for humans and animals, and is closely related to the health of the body. However, in actual production, for example, inorganic selenium-sodium selenite is mainly added in the feed of livestock and poultry, which has strong toxic effects and low bioavailability, which easily causes environmental pollution. In addition, the dosage of inorganic selenium is difficult to control, and young animals are more sensitive to selenium, which can easily lead to poisoning. Compared with inorganic selenium, organic selenium is less toxic and has a relatively higher absorption rate. However, the subchronic toxicity of the two is similar. At the same time, the production process ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/245
CPCC12P3/00
Inventor 徐春兰乔磊郭宇马丽程忆忆
Owner NORTHWESTERN POLYTECHNICAL UNIV
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