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Detection method, detection kit and application of fmo3 gene SNP related to tia susceptibility

A kit and susceptibility technology, applied in biochemical equipment and methods, microbial determination/test, DNA/RNA fragments, etc., can solve problems such as unreported relationship, and achieve the effect of reducing the risk of drug development

Active Publication Date: 2021-07-13
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Different SNPs on the FMO3 gene of human chromosome 1 (1q24.3) are related to individual fishy smell syndrome and drug metabolism (Gong Zheng, Wang Qi. Gene polymorphism of flavin monooxygenase 3 and its role in drug The role in metabolism and toxicity [J]. Chinese Journal of Traditional Chinese Medicine, 2015, 40(14): 2701-2705.), but there is no report on the relationship between FMO3 (flavin monooxygenase 3) gene SNP and TIA

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  • Detection method, detection kit and application of fmo3 gene SNP related to tia susceptibility
  • Detection method, detection kit and application of fmo3 gene SNP related to tia susceptibility
  • Detection method, detection kit and application of fmo3 gene SNP related to tia susceptibility

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with the examples, which are used to explain the content of the invention, but not to limit the present invention.

[0028] (1) Blood sample collection and genomic DNA extraction

[0029] The steps for preparing genomic DNA from human peripheral blood are as follows:

[0030] (1) Thaw 1 mL of anticoagulated stored frozen blood into a centrifuge tube after thawing at room temperature, add 1 mL of phosphate buffered saline (PBS), mix well, centrifuge at 12,000 rpm for 10 min (4°C), pour off the supernatant containing lysed red blood cells; repeat once.

[0031] (2) Suspend the white blood cell pellet with 500 μL of DNA extraction solution, incubate in a water bath at 37°C for 1 hour, add 10 μL of 10 mg / mL proteinase K, and mix evenly by rotating up and down, the liquid becomes viscous. Cells were lysed and proteins digested in a water bath at 56°C overnight. During the heat preservation process, turn ...

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Abstract

The invention provides a method for detecting FMO3 gene SNP related to TIA susceptibility, a detection kit and application thereof. Utilize the specific nucleic acid primers shown in SEQ.ID.NO.2 and SEQ.ID.NO.3, and the UEP primers shown in SEQ.ID.NO.4 to amplify the SNP site rs1736557, using the matrix The SNP site rs1736557 is genotyped by assisted laser desorption ionization time-of-flight mass spectrometry, and the invention also relates to a detection kit for the SNP site rs1736557. The invention provides a new approach for the identification of TIA susceptibility, and provides an experimental basis for the selection of clinical test subjects of antithyroid drugs.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to a FMO3 gene single nucleotide polymorphism (single nucleotide polymorphism, SNP) site rs1736557 related to susceptibility to antithyroid drug-induced agranulocytosis (TIA) and its application. Background technique [0002] Hyperthyroidism is a general term for diseases characterized by increased excitability and hypermetabolism in the nervous, circulatory, and digestive systems caused by excessive thyroid hormones produced by the thyroid gland, referred to as hyperthyroidism. An epidemiological survey in my country in 2010 showed that the prevalence of hyperthyroidism was 1.1%. There are three main treatment methods, including antithyroid drugs (Antithyroid drug, ATD), radioactive iodine therapy 131 I and surgical treatment. All three methods can reduce the synthesis of thyroid hormone, so as to achieve the purpose of treating hyperthyroidism. At present, antithyroid drugs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 张宝龚晓娟贺雅毅张倩阎春霞李少卿杨婧思
Owner XI AN JIAOTONG UNIV