Method for measuring phthalates in flavors and fragrances for tobaccos

Abstract

The invention discloses a method for measuring phthalates (PAEs) in flavors and fragrances for tobaccos. The method comprises the following steps: dispersing and initially extracting samples with an aqueous ethanol solution at a certain concentration; then using a mixed solvent of n-hexane/ethyl acetate/isopropanol at a certain ratio for liquid-liquid extraction of an initial extract; directly sampling supernatant for analysis after layering through liquid-liquid extraction. The method provided by the invention can disperse most flavor and fragrance samples well in the initial extraction step,and uses the mixed solvent of n-hexane/ethyl acetate/isopropanol for the liquid-liquid extraction of the initial extract to not only purify the initial extract, but also make a recovery rate of 18 PAEs reach 90% or higher. The method uses fewer glass instruments, uses an internal standard method to further simplify a solution transfer step and greatly reduce probability of contamination during pretreatment, and is suitable for measuring mass samples.

Description

technical field [0001] The invention belongs to the field of physical and chemical analysis of harmful components of tobacco additives, and is suitable for the determination of phthalates in tobacco flavors and fragrances (liquid, paste and milk). Background technique [0002] Tobacco flavor is a kind of food flavor. Adding flavors in the process of making cigarettes can neutralize, correct, or change the taste and mouthfeel of tobacco to some extent to achieve a pleasant effect. However, tobacco flavors are not only used for flavoring shredded tobacco, but are also used on cigarette packaging materials (such as cigarette paper, filter rods, etc.) to meet the olfactory needs of consumers. It can be said that when consumers consume cigarette products, they have a very large chance of being directly exposed to tobacco flavors. At present, the safety and hygiene requirements for tobacco flavors mainly refer to food flavors. [0003] Phthalates (PAEs) are one of the controlle...

AI Technical Summary

Problems solved by technology

There is a recommended method for the determination of tobacco flavor in the tobacco industry, but this method uses ethanol to directly dilute the sample, uses GC-MS for measurement, and has no impurity removal steps, so there are many problems in use

On the one hand, many flavors or spices are insoluble in ethanol (such as some extracts), and on the other hand, the flavor components are complex, and direct injection will pollute the analytical column and ion source, and the matrix effect is obvious

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