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Diterpene alkaloid compound in delphinium pseudoaemulans C.Y.Yang etB.Wang and preparation method and application thereof

A technology of diterpene alkaloids and compounds, applied in the field of phytochemistry, can solve the problems that the chemical composition has not been reported yet

Active Publication Date: 2018-10-23
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Saitacheng Delphinium, born in Saul Mountain, 1900 meters above sea level, hillside grassland, shrubs and wetlands in river valleys, produced in Bukser County, is a unique plant in Xinjiang, with obvious regional specificity, but its chemical composition Not yet reported

Method used

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  • Diterpene alkaloid compound in delphinium pseudoaemulans C.Y.Yang etB.Wang and preparation method and application thereof
  • Diterpene alkaloid compound in delphinium pseudoaemulans C.Y.Yang etB.Wang and preparation method and application thereof
  • Diterpene alkaloid compound in delphinium pseudoaemulans C.Y.Yang etB.Wang and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Take 10.0 kg of the whole herb of Delphinium delphinium Saitacheng, crush it, extract it with 50 L of ethanol with a volume fraction of 80% by percolation at room temperature, evaporate the solvent to dryness under reduced pressure, and obtain the crude extract of Delphinium delphinium Saitacheng;

[0069] The obtained crude extract was treated with 2% H 2 SO 4 dispersed, the resulting acidic aqueous layer was washed with Na 2 CO 3 Adjust the pH to 10, then extract with chloroform, and evaporate the chloroform to dryness under reduced pressure to obtain the total alkaloid extract;

[0070] The obtained total alkaloid extract is used for silica gel column chromatography, and the filler is a normal phase silica gel column for separation, with a volume ratio of 100:1, 80:1, 60:1, 40:1, 20:1, 10:1, 5:1, 2:1, 1:1 and 0:1 chloroform and methanol were used as eluents for gradient elution, and the fractions were analyzed by silica gel thin layer chromatography (TLC). The sam...

Embodiment 2

[0075] Take 10.0kg of the whole plant of Delphinium delphinium Saitacheng, crush it and extract it with 50L of 95% ethanol under reflux at room temperature, evaporate the solvent to dryness under reduced pressure, and obtain the crude extract of Delphinium delphinium Saitacheng;

[0076] The crude extract extract that obtains is that the volume concentration is 3% H 2 SO 4 Disperse, adjust the pH of the obtained acidic water layer to 10 with ammonia water, extract with ether, and evaporate the ether to dryness under reduced pressure to obtain the total alkaloid extract;

[0077] The obtained total alkaloid extract is separated with a normal phase silica gel column, with a volume ratio of 100:1, 80:1, 60:1, 40:1, 20:1, 10:1, 5:1, 2:1, 1:1 and 0:1 chloroform-methanol were used as the eluent for gradient elution, and the fractions were analyzed by silica gel thin-layer chromatography (TLC), and the same fractions were combined to obtain 6 fractions A-F;

[0078] The obtained B ...

Embodiment 3

[0082] Take 10.0kg of the whole herb of Delphinium delphinium Saitacheng, crush it and extract it with 50L of 40% ethanol percolation at room temperature, evaporate the solvent to dryness under reduced pressure, and obtain the crude extract of Delphinium delphinium Saitacheng;

[0083] The obtained crude extract was treated with 5% H 2 SO 4 dispersed, the resulting acidic aqueous layer was washed with Na 2 CO 3 Adjust the pH to 10, extract with ethyl acetate, and evaporate the ethyl acetate to dryness under reduced pressure to obtain the total alkaloid extract;

[0084] The obtained total alkaloid extract is separated with a normal phase silica gel column, with a volume ratio of 100:1, 80:1, 60:1, 40:1, 20:1, 10:1, 5:1, 2:1, 1:1 and 0:1 chloroform-methanol were used as the eluent for gradient elution, and the fractions were analyzed by silica gel thin-layer chromatography (TLC), and the same fractions were combined to obtain 6 fractions A-F;

[0085] The obtained component...

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Abstract

The invention relates to a diterpene alkaloid compound in delphinium pseudoaemulans C.Y.Yang etB.Wang and a preparation method and application thereof. The preparation method comprises the following steps of using the pseudoaemulans C.Y.Yang etB.Wang as the raw material, extracting by a solvent, treating by acid and alkaline, extracting by a solvent, separating by two, three or four types of silica gel column chromatography, thin layer preparation chromatography, glucan gel LH-20 column chromatography or high performance liquid chromatography, detecting and analyzing by thin layer chromatography or high performance liquid chromatography, so as to obtain the novel diterpene alkaloid compound. The determination of the in-vitro tumor cell toxicity is performed on the diterpene alkaloid compound. Proofed by experiment results, the diterpene alkaloid compound separated from the delphinium pseudoaemulans C.Y.Yang etB.Wang can show different antitumor activity on human lung cancer A549 cellsand human cervical cancer Hela cells, and can be used for preparing antitumor medicines.

Description

technical field [0001] The invention relates to the field of phytochemistry, in particular to a diterpenoid alkaloid compound from Delphinium delphinium in Saita City in plant medicinal materials, a preparation method and an application. Background technique [0002] Delphinium (Delphinium) belongs to Ranunculaceae (Ranunculecae) ​​plants, there are more than 300 species in the world, widely distributed in the northern temperate zone, about 140 species in my country, except for Taiwan and Hainan provinces, are distributed all over the country, southwest and northwest are Its main distribution area, Xinjiang has 26 species and 3 varieties. There are 18 species of plants in this genus in my country that are used for medicinal purposes among the people to treat bruises, rheumatism, toothache, enteritis and other diseases. Another 4 are used as soil pesticides to kill lice, mosquitoes and fly larvae. [0003] The qualitative chemical components in this genus include diterpene a...

Claims

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Application Information

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IPC IPC(8): C07D471/08C07D491/18A61P35/00
CPCA61P35/00C07B2200/07C07D471/08C07D491/18
Inventor 阿吉艾克拜尔·艾萨薛文娟赵波
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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