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Temperature sensitive hydrogel composition including nucleic acid and chitosan

A temperature-sensitive hydrogel and composition technology, which is applied in the directions of medical preparations containing active ingredients, drug delivery, organic active ingredients, etc., can solve problems such as composition differences, and achieve the effect of improving retention and adhesion time.

Active Publication Date: 2018-10-23
PHARMARES PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] European Published Patent No. 2745849 shows the combination of polydeoxyribonucleotide (PDRN) and chitosan, but does not refer to thermosensitive hydrogels containing 0.002 to 0.25% by weight of polydeoxyribonucleotides And 1 to 10% by weight chitosan, it can be seen that there are differences with the composition of the present invention

Method used

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  • Temperature sensitive hydrogel composition including nucleic acid and chitosan
  • Temperature sensitive hydrogel composition including nucleic acid and chitosan
  • Temperature sensitive hydrogel composition including nucleic acid and chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059]

[0060] In order to prepare the nucleic acid-chitosan hydrogel, the nucleic acid and chitosan stock solutions (stock solutions) of the concentrations corresponding to the examples in the following Table 1 were prepared. At this time, nucleic acid was added to 200 mM sodium phosphate dibasic dodecahydrate buffer solution, and dissolved using a heating stirrer at 65° C. for 1 hour or more.

[0061] Chitosan was dissolved with 100 mM acetic acid.

[0062] The nucleic acid and chitosan stock solutions prepared according to the concentrations in Table 1 below were mixed at a weight ratio of 1:1, and stirred in a heating stirrer at 60° C. for 1 hour. Afterwards, the temperature was lowered to normal temperature and stirred for 1 hour, thereby preparing the nucleic acid-chitosan hydrogel.

Embodiment 2

[0067]

[0068] Nucleic acid-chitosan-hyaluronic acid hydrogels were prepared by the following procedure.

[0069]The nucleic acid was dissolved in a 200 mM sodium phosphate dibasicdodecahydrate buffer solution to a concentration of 2.2% by weight. At this time, it was melt|dissolved using the heating stirrer of 65 degreeC for 1 hour or more.

[0070] Chitosan was dissolved in 100 mM acetic acid to a concentration of 0.4% by weight.

[0071] Sodium hyaluronate was dissolved in 200 mM sodium phosphate dibasicdodecahydrate buffer solution so as to have a concentration of 2% by weight. At this time, after dissolving for 30 minutes using a heating stirrer at 40° C., the temperature was lowered to normal temperature while stirring.

[0072] The prepared 2.2 wt% nucleic acid and 0.4 wt% chitosan solution were mixed at a weight ratio of 9:1, and stirred in a heating stirrer at 65° C. for 10 minutes. In the nucleic acid-chitosan mixed solution, further add 2% by weight of sodium ...

experiment example 1

[0080]

[0081] The hydrogel compositions of Example 1, Example 2, Comparative Example 1, and Comparative Example 2 were used to verify gelation, gel stability, and solubility.

[0082] After mixing each composition, observe the transparency and gelation state of each composition for three days with the naked eye. The gelation is verified by whether there is viscoelasticity, and the stability of the gel is verified by whether there is a precipitate and whether stratification occurs. to verify.

[0083] In terms of gel solubility, when the hydrogel compositions of Example 1, Example 2, Comparative Example 1, and Comparative Example 2 were titrated in an aqueous solution at 37.5°C to gel, and then maintained at 37.5°C Stir at 400 rpm for 5 minutes while confirming whether the gel is dissolved. The results are shown in Table 3, figure 1 and figure 2 .

[0084] [table 3]

[0085]

[0086] From the table 3 and figure 1 It can be seen that the temperature-sensitive hydro...

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Abstract

The present invention provides a temperature sensitive hydrogel composition including a nucleic acid and chitosan. Since the hydrogel has excellent biocompatibility and biostability, and simultaneously has sol-gel phase transition properties depending on temperature changes, the hydrogel is present in a sol state at room temperature and becomes a gel when the hydrogel is injected into the human body or applied on the surface of epithelial skin and the temperature increases. Thus, the temperature-sensitive hydrogel of the present invention can be directly injected into and applied on certain parts requiring treatment and the retention and attaching time of a drug is increased through gelation depending on the temperature so that drug efficacy is sufficiently exhibited. Therefore, it is expected that the temperature-sensitive hydrogel of the present invention can be utilized for various treatments.

Description

technical field [0001] The invention relates to a thermosensitive hydrogel composition comprising nucleic acid and chitosan. Background technique [0002] Hydrogel refers to a polymer structure with a three-dimensional network structure containing an aqueous phase (Rohindra D.R. et al., 2004), through the formation of covalent bonds or non-covalent bonds between hydrophilic polymers. valence bonds to form (HwangJunseok et al., 2008). Since hydrogels composed of various hydrophilic polymers have high water content and excellent biocompatibility, many researches have been conducted on their application as biomaterials. Hydrogels that are particularly sensitive to stimuli such as pH, temperature, electric field, magnetic field, light, ultrasound, etc., can be used in drug delivery systems with controlled release depending on the presence or absence of stimuli (Hoffman A.S., 2002). [0003] Among them, as far as thermosensitive hydrogels are concerned, they are most widely stu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36C08J3/075A61K31/7088C08L5/08
CPCA61K47/36A61K31/7088C08J3/075C08L5/08C08J2377/04A61K9/0024C08J2405/08C08J2305/00C08J2305/08C08L5/00
Inventor 金益洙金汉奎洪铁岩李修渊
Owner PHARMARES PROD
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