Molecular marker method of growth characteristics of pigs
A technology for molecular markers and growth traits, which is applied in the field of molecular markers for pig growth traits, and can solve problems such as unclear mechanisms
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Embodiment 1
[0048] The ear margin tissues of 390 large Yorkshire pigs were collected, and their growth rate was measured at the same time, that is, the age at 100 kg body weight. Take it back to the laboratory, and carry out the genotype detection of the molecular marker CALB2 gene of pig growth traits respectively. The specific operation steps are as follows:
[0049] (1) Extracting the genomic DNA of the Great Yorkshire pig
[0050] Extraction of porcine genomic DNA from ear margin tissue of Large Yorkshire pigs;
[0051] (2) Primer design
[0052] A pair of primers were designed between -1201bp and -654bp of the start transcription site of the pig calcium binding protein 2 (CALB2) gene, that is, the upstream primer and the downstream primer. The sequences of the upstream primer and the downstream primer are as follows:
[0053] CALB2-F upstream primer: 5'-TGGTGTTCCTCTCTCCCCTG-3'
[0054] CALB2-R downstream primer: 5'-CCACTGTCCTCTCGTGTTGA-3';
[0055] (3) Polymerase chain reaction ...
Embodiment 2
[0079]The ear margin tissues of 235 Duroc pigs were collected, and their growth rate was measured at the same time, that is, the age at 100 kg body weight. Take it back to the laboratory, and carry out the genotype detection of the molecular marker CALB2 gene of pig growth traits respectively. The specific operation steps are as follows:
[0080] (1) Genomic DNA extraction of Duroc pigs
[0081] Extraction of porcine genomic DNA from ear margin tissues of Duroc pigs;
[0082] (2) Primer design
[0083] A pair of primers were designed between -1201bp and -654bp of the start transcription site of the pig calcium binding protein 2 (CALB2) gene, that is, the upstream primer and the downstream primer. The sequences of the upstream primer and the downstream primer are as follows:
[0084] CALB2-F upstream primer: 5'-TGGTGTTCCTCTCTCCCCTG-3'
[0085] CALB2-R downstream primer: 5'-CCACTGTCCTCTCGTGTTGA-3';
[0086] (3) Polymerase chain reaction
[0087] A. Preparation of polymerase...
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