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Molecular marker method of growth characteristics of pigs

A technology for molecular markers and growth traits, which is applied in the field of molecular markers for pig growth traits, and can solve problems such as unclear mechanisms

Active Publication Date: 2018-10-26
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Expression of calbindin 3, like that of calbindin 1, is activated by the active vitamin D metabolite calcitriol, but the exact mechanism remains unclear

Method used

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  • Molecular marker method of growth characteristics of pigs
  • Molecular marker method of growth characteristics of pigs
  • Molecular marker method of growth characteristics of pigs

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Effect test

Embodiment 1

[0048] The ear margin tissues of 390 large Yorkshire pigs were collected, and their growth rate was measured at the same time, that is, the age at 100 kg body weight. Take it back to the laboratory, and carry out the genotype detection of the molecular marker CALB2 gene of pig growth traits respectively. The specific operation steps are as follows:

[0049] (1) Extracting the genomic DNA of the Great Yorkshire pig

[0050] Extraction of porcine genomic DNA from ear margin tissue of Large Yorkshire pigs;

[0051] (2) Primer design

[0052] A pair of primers were designed between -1201bp and -654bp of the start transcription site of the pig calcium binding protein 2 (CALB2) gene, that is, the upstream primer and the downstream primer. The sequences of the upstream primer and the downstream primer are as follows:

[0053] CALB2-F upstream primer: 5'-TGGTGTTCCTCTCTCCCCTG-3'

[0054] CALB2-R downstream primer: 5'-CCACTGTCCTCTCGTGTTGA-3';

[0055] (3) Polymerase chain reaction ...

Embodiment 2

[0079]The ear margin tissues of 235 Duroc pigs were collected, and their growth rate was measured at the same time, that is, the age at 100 kg body weight. Take it back to the laboratory, and carry out the genotype detection of the molecular marker CALB2 gene of pig growth traits respectively. The specific operation steps are as follows:

[0080] (1) Genomic DNA extraction of Duroc pigs

[0081] Extraction of porcine genomic DNA from ear margin tissues of Duroc pigs;

[0082] (2) Primer design

[0083] A pair of primers were designed between -1201bp and -654bp of the start transcription site of the pig calcium binding protein 2 (CALB2) gene, that is, the upstream primer and the downstream primer. The sequences of the upstream primer and the downstream primer are as follows:

[0084] CALB2-F upstream primer: 5'-TGGTGTTCCTCTCTCCCCTG-3'

[0085] CALB2-R downstream primer: 5'-CCACTGTCCTCTCGTGTTGA-3';

[0086] (3) Polymerase chain reaction

[0087] A. Preparation of polymerase...

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Abstract

The invention relates to a molecular marker method of growth characteristics of pigs. The molecular marker method comprises the following operation steps: extracting genome DNA (Deoxyribonucleic Acid)of the pigs from ear marginal tissues of the pigs; designing one pair of primers from -1201bp to -654bp of a transcription start site of calcium-binding protein 2 (CALB2) of the pigs, i.e., an upstream primer and a downstream primer; carrying out polymerase chain reaction to obtain an in vitro amplification product with the length of 548bp from the -1201bp to the -654bp of the transcription startsite of the CALB2 of the pigs; carrying out restriction enzyme HhaI enzyme digestion reaction on the in vitro amplification product; detecting the restriction fragment length polymorphism (RLFP) of an enzyme digestion product, so as to obtain a strip AA type gene type with a good growth speed, i.e., a mutation type; selecting strip AA type piglets for breeding, wherein the growth and fattening period of the strip AA type pigs is shortened by 3.6 to 4.0 days when being compared with that of GG type pigs.

Description

technical field [0001] The invention belongs to the technical field of pig breeding, and in particular relates to a molecular marker method for growth traits of pigs. Background technique [0002] Growth rate is an important indicator that affects the economic benefits of pig producers. The shorter the feeding cycle of pigs with faster growth rate, the lower the cost required, and the higher income pig producers can obtain. The traditional breeding of pig growth speed is to measure the weight of pigs one by one when they grow to 85kg to 130kg. At this time, the age is about 150-180 days. Then select and retain, time-consuming, labor-intensive, and capital-consuming. [0003] Calbindin refers to the general term for several calcium-dependent binding proteins. It was originally a vitamin D-dependent calcium-binding protein found in the kidney and small intestine of chickens and mammals. Because of the hand-shaped domain of calcium-binding protein ( EF-hand) is divided into d...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 李庆岗吴义景苏世广杨家军
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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