Device and method for detecting L-carnitine in infant milk powder based on liquid chromatography-mass spectrometry combination

Abstract

The invention relates to a device for detecting L-carnitine in infant milk powder based on liquid chromatography-mass spectrometry combination. The device comprises a container and further comprises abottom plate, a sliding rail and a detection mechanism, wherein the sliding rail is fixedly arranged on the bottom plate and is used for transferring the container; the detection mechanism is fixedlyarranged on the bottom plate and positioned on the lateral direction of the sliding rail; the detection mechanism comprises a plurality of liquid measuring devices, a sample measuring device, a centrifugal machine, a hot bath device, a cold bath device and a mass spectrometer which are arranged sequentially. According to the corresponding detection method, the quantity of the liquid measuring devices is four; ultrapure water is stored in the first liquid measuring device; an acetonitrile aqueous solution is stored in the second liquid measuring device; a potassium hydroxide aqueous solution is stored in the third liquid measuring device; a hydrochloric acid aqueous solution is stored in the fourth liquid measuring device. The detection method comprises a sample treatment stage and a massspectrometric analysis stage. Various reagents and raw materials for detection can be measured rapidly, so that the detection efficiency is improved and accurate detection result is obtained.

Description

technical field [0001] The invention relates to the technical field of milk powder detection, in particular to a device and method for detecting L-carnitine in infant milk powder based on liquid chromatography-mass spectrometry. Background technique [0002] Carnitine is a class of amino acids belonging to quaternary ammonium cationic complexes that can be biosynthetically produced from lysine and methionine. Carnitine has two isomers: the biologically active L-carnitine and the non-biologically active enantiomer D-carnitine. L-carnitine is mainly distributed in muscle and heart, and can transport long-chain fatty acids into mitochondria, where oxygenation occurs to generate energy. Humans can not only obtain L-carnitine through food, but also synthesize it in the body. However, infants and young children have low self-synthesizing ability, so it is necessary to supplement L-carnitine in infant food. my country stipulates that if L-carnitine is added to formula foods for o...

AI Technical Summary

Problems solved by technology

Due to the complex matrix and large interference of infant formula milk powder, it is difficult to measure accurately

[0004] At the same time, because the detection process requires frequent measurement and transfer of materials, the shaking of the hand-held container during the movement can easily cause the material to adhere to the inner wall of the container, which in turn leads to inaccurate detection results. In order to obtain accurate results, it is necessary to repeat it many times. testing, but this requires repeated measuring process, heavy workload, affecting the speed

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