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A feed additive containing lysozyme

A technology of feed additive and lysozyme, which is applied in the field of feed, can solve the problems of few microbial lysozymes, limited application, and limited application range, etc.

Active Publication Date: 2021-07-06
浙江伊杰斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the most common lysozyme on the market is egg white lysozyme, but it only has an effect on Gram-positive bacteria, which limits its application range
In recent years, extensive research on lysozyme from various sources has been carried out at home and abroad, but there are few research reports on the preparation of microbial lysozyme by fermentation methods, resulting in limited application of this type of non-egg white lysozyme in actual industrial production

Method used

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  • A feed additive containing lysozyme
  • A feed additive containing lysozyme
  • A feed additive containing lysozyme

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L. Get 25mL liquid culture medium and place in Erlenmeyer flask, insert the thalline seed of Streptomyces griseus S-35 by the inoculum size of 4% (v / v), carry out shaking flask fermentation, fermentation condition is 30 ℃, 200r / min, After 24 hours of fermentation and cultivation, the fermentation liquid was centrifuged to remove the bacteria, and the supernatant was successively salted out with ammonium sulfate (add ammonium sulfate to the supernatant to 20% saturation, stirred for 1 hour, then stood still for 1 hour, centrifuged at 10,000 r / min, precipitated Dissolve it in distilled water, put it into a dialysis bag with a molecular weight of 10,000, and place it in 10mmol / LPBS for full dialysis; add ammonium sulfate to the supernatant in the dialysis bag to 70% saturation, stir for 1 hour, let it stand for 1 hour, and centrifuge at 10,000r / min. The precipitate was dialyzed in the same way as above to obt...

Embodiment 2

[0030] Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L. Get 25mL liquid culture medium and place in Erlenmeyer flask, insert the thalline seed of Streptomyces griseus S-35 by the inoculum size of 4% (v / v), carry out shaking flask fermentation, fermentation condition is 20 ℃, 150r / min, After 24 hours of fermentation and cultivation, the fermentation liquid was centrifuged to remove the bacteria, and the supernatant was successively salted out with ammonium sulfate (add ammonium sulfate to the supernatant to 20% saturation, stirred for 1 hour, then stood still for 1 hour, centrifuged at 10,000 r / min, precipitated Dissolve it in distilled water, put it into a dialysis bag with a molecular weight of 10,000, and place it in 10mmol / LPBS for full dialysis; add ammonium sulfate to the supernatant in the dialysis bag to 70% saturation, stir for 1 hour, let it stand for 1 hour, and centrifuge at 10,000r / min. Take the precipitate and dialyze, the method is the same as ...

Embodiment 3

[0032]Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L. Get 25mL liquid culture medium and place in Erlenmeyer flask, insert the thalline seed of Streptomyces griseus S-35 by 4% (v / v) inoculum size, carry out shaking flask fermentation, fermentation condition is 40 ℃, 300r / min, After 24 hours of fermentation and cultivation, the fermentation liquid was centrifuged to remove the bacteria, and the supernatant was successively salted out with ammonium sulfate (add ammonium sulfate to the supernatant to 20% saturation, stirred for 1 hour, then stood still for 1 hour, centrifuged at 10,000 r / min, precipitated Dissolve it in distilled water, put it into a dialysis bag with a molecular weight of 10,000, and place it in 10mmol / LPBS for full dialysis; add ammonium sulfate to the supernatant in the dialysis bag to 70% saturation, stir for 1 hour, let it stand for 1 hour, and centrifuge at 10,000r / min. The precipitate was dialyzed, and the dialysate was obtained in the ...

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Abstract

The invention relates to a feed additive containing lysozyme, wherein the lysozyme is prepared by a fermentation process, and the specific steps are as follows: take a liquid culture medium and place it in a conical flask, and insert Streptomyces griseus at an inoculation amount of 4% v / v Shake-flask fermentation was carried out on the bacterial seeds of the bacterium, and the fermentation conditions were 20-40°C, 150-300r / min. After 24 hours of fermentation and cultivation, the fermentation liquid was centrifuged to remove the bacterium, and the supernatant was sequentially used by ammonium sulfate salting-out method, coagulation After purification by gel column chromatography and cation exchange column chromatography, it can be obtained by freeze-drying. The lysozyme feed additive obtained by using the above fermentation process has a good preventive effect on piglet diarrhea, and the effect is better when used together with the traditional Chinese medicine extract.

Description

technical field [0001] The invention relates to the field of feed, in particular to a feed additive containing lysozyme. Background technique [0002] In recent years, with the highly intensive production of the animal husbandry industry, the incidence of livestock and poultry diseases has also increased day by day, and the mortality rate due to diseases has increased year by year, causing major economic losses to the animal husbandry industry. Diarrhea is one of the main factors affecting the survival rate and growth of piglets. The traditional prevention and treatment measures are the use of antibiotics. Although the use of antibiotics in livestock and poultry feed has played an important role in effectively controlling diseases, improving feed conversion rate, and ensuring the stable supply of livestock products, due to the over-range, high-dose or multi-species, low-dose antibiotics in feed production for a long time The use, as well as the abuse and misuse of antibioti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K20/189A23K10/30A23K50/30A61K38/47A61P1/12A61K36/71
CPCA61K38/47A61K36/71C12Y302/01017A61K2236/53A61K2236/39A61K2236/331A23K50/30A23K50/60A23K10/12A23K20/189A23K10/30A61P1/12C12N9/2462A61K2236/51A61K2300/00Y02A40/70
Inventor 潘宏涛卢亚萍
Owner 浙江伊杰斯生物科技有限公司
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