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Euglena culture medium

A technology of culture medium and liquid culture medium, applied in the direction of unicellular algae, microorganism-based methods, microorganisms, etc., can solve the problems of high production cost and low fatty acid yield, etc.

Inactive Publication Date: 2018-11-02
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, microalgae biodiesel has made great progress at home and abroad, but still faces many challenges, such as high production cost and low yield of fatty acids, etc. These problems have become the bottleneck of further large-scale production of microalgae biodiesel

Method used

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Examples

Experimental program
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example 1

[0026] Example 1: Euglena slenderum ( Euglena gracilis ) by nitrosoguanidine (NTG) mutagenesis breeding and the acquisition of mutants

[0027] 1) Prepare PolyA medium according to the formula in Table 1

[0028] Table 1 PolyA medium for culturing in the present invention

[0029]

[0030] In the present invention, the solid PolyA medium is composed of 2 g of sodium acetate, 1 g of tryptone, 1 g of yeast extract, 10 g of agar powder, and water to 1 L.

[0031] 2) Preparation method of PBS solution

[0032] The PBS solutions of the present invention are all phosphate buffers of 0.2 mol / L and pH 7.0: from 0.2 mol / L Na 2 HPO 4 61mL with 0.2mol / L NaH 2 PO 4 39mL mixed.

[0033] 3) The preparation method of the 0.8mg / ml nitrosoguanidine (NTG) solution of the present invention

[0034] Consists of 1.6ml of 0.2M PBS (pH7.0) mixed with 4.0mg / ml NTG.

[0035] Prepare Heter liquid medium according to the formula in Table 2

[0036] Table 2 Heter liquid medium for culturin...

example 2

[0049] Example 2: Comparison of wild-type Euglena gracilis (WT) and mutant strains A4 and A11

[0050] 1. Comparison of growth rates

[0051] The obtained mutant strains A4 and A11 of Euglena slenderness were cultured in Heter liquid medium for 3-4 days, and inoculated into new Heter liquid medium at a volume ratio of 1:10, so that the number of cells after inoculation was 2.0×105 1 / ml, three parallel samples were taken for each group, 1ml of algal fluid was taken every day for hemocytometer counting, and the growth curve was drawn. In this step, the conditions are all light 65±5μmol / m 2 s 1 , 24h light, temperature 25±1℃. Comparison of the growth of wild-type Euglena slenderness (WT) and mutant strains A4 and A11 figure 2 shown.

[0052] 2. Comparison of fatty acid content

[0053] 1) Extraction of fatty acids

[0054] The algal strain is cultured in a culture flask, and the growth plateau phase is reached after 9 days of culture. During this period, the accumulatio...

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Abstract

The invention discloses an euglena mutant, a preparation method and an euglena culture medium. A collection number of the euglena mutant is CCAP (Culture Collection of Algae and Protozoa) 1224 / 5Z. Wild euglena gracilis is treated a chemical reagent, namely nitrosoguanidine (NTG); an undirectional variation occurs; enlarged cultivation is performed; the euglena mutant growing quickly and having a high fatty acid content is screened out by a directional screening method; and the collection number of the mutant is CCAP 1224 / 5Z. The euglena mutant with a high production rate and the high fatty acid content is screened out by a chemical reagent nitrosoguanidine (NTG) mutation breeding technology combined with the directional screening method.

Description

technical field [0001] The invention belongs to the field of Euglena culturing, in particular to a medium for Euglena slenderness with high production rate and high fatty acid yield. Background technique [0002] In recent years, the world is facing the increasing depletion of fossil energy, and people have begun to seek new alternative energy sources. Biodiesel is the only energy that can replace petroleum fuel on a large scale, so it has received extensive attention. Compared with petroleum diesel, biodiesel has the characteristics of non-polluting the environment and being renewable. Among them, microalgal biodiesel is a kind of biomass energy with great development potential. Compared with traditional biodiesel, microalgal biodiesel has more advantages, such as easy cultivation, no arable land occupation, and high oil yield. , rapid reproduction, etc. [0003] With the increasing pressure of energy depletion and environmental deterioration in human society, the use o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 陆嘉欣杨磊王江新
Owner SHENZHEN UNIV
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