Application of At3g16910 genes in cultivation of drought-resistant crops
An at3g16910, gene technology, applied in the application field of Arabidopsis thaliana At3g16910 gene in cultivating drought-resistant crops, can solve problems such as no research report, no research, etc.
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Embodiment 1
[0027] Arabidopsis At3g16910 The CDS length of the gene is 1710bp, which encodes a protein of 569 amino acids. It belongs to the acyl activating enzyme family gene. Existing studies have shown that this gene is related to fatty acid metabolism in peroxisomes, but there is no relationship between this gene and ABA-regulated lateral root development. and research reports on stomatal movement.
[0028] In the present invention, the inventor believes that Arabidopsis At3g16910 Genes are highly associated with ABA-regulated lateral root development and stomatal movement, through the identification of Arabidopsis At3g16910 Gene mutants (including functional loss and overexpression mutants) and wild-type comparative studies, the inventors believe that by using genetic engineering means, by using At3g16910 Gene overexpression can be used to breed new varieties of drought-tolerant crops.
[0029] This example mainly introduces Arabidopsis At3g16910 The discovery of genes that are h...
Embodiment 2
[0035] On the basis of Example 1, for further verification At3g16910 gene function, the inventors used genetic engineering technology to At3g16910 Genes were retransformed into Arabidopsis mutants separately rsa1-1 and wild-type (WT) plants, constructed transgenic homozygous plants com1 and OE1 , the build process is briefly described below.
[0036] homozygous plant com1 ( At3g16910 Gene function complementing plant) prepared
[0037] Cloned from Arabidopsis wild-type (WT) plant DNA by PCR At3g16910 The promoter and gene sequence of the gene, that is, according to the Arabidopsis At3g16910 The full-length genomic DNA sequence of the gene, plus the 1600 bp upstream promoter region, designed specific amplification primers to amplify At3g16910 The full-length sequence of the gene. The specific construction process is as follows.
[0038] , PCR amplification At3g16910 Gene fragment
[0039] The designed primer sequences are as follows (as shown in SEQ ID NO.1~2): ...
Embodiment 3
[0111] This example mainly introduces Arabidopsis At3g16910 Relationship between genes and the regulation of stomatal movement.
[0112] Arabidopsis wild type (WT), mutant ( rsa1-1 and rsa1-2 ), homozygous plants com1 , homozygous plants OE1 The seeds were planted on 0.6% agar MS medium respectively, and germinated and grown for 10 days in the light culture room with a light / dark cycle of 12 / 12h, a temperature of 22°C and a relative humidity of 70%, and then transplanted to the same condition 18 days in the nutrient soil below.
[0113] At the beginning of the light, cut the flat and unfolded leaves respectively, suspend the leaves flatly (the lower epidermis is in contact with the liquid surface) in the stomatal buffer solution for 5 hours, then carefully and quickly tear off the lower epidermis of the leaves with tweezers, and take pictures under a 40X microscope And count the stomatal opening.
[0114] The statistical results show that the mutant rsa1-1 Stomatal...
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