Method for determining content of index component derived from herba artemisiae annuae in reduning injection
A determination method and injection technology, applied in the field of analytical chemistry, to achieve the effects of simple operation, increased quality control, and accurate analysis data
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0069] Examples 1-5 Quantitative detection method of index components derived from Artemisia annua in Reduning injection
[0070] Preparation of standard solution: take scopoletin and (1S,6R,7R,10R)-6-carboxy-10-methyl-α-methylene-1-(1-oxobutyl)-cyclohexane Acrylic acid reference substance, add methanol solution with a volume concentration of 50% (methanol and purified water are prepared according to the volume ratio of 1:1) to prepare mixed reference substance stock solutions with concentrations of 4.17 and 19.50 μg / mL, respectively, 0.22 μm microporous membrane filtration.
[0071] Preparation of the solution to be tested: Accurately measure 1 mL of Reduning Injection and place it in a 10 mL volumetric flask, add methanol solution with a volume concentration of 50% to dilute to the mark, shake well, and filter with a 0.22 μm microporous membrane to obtain the solution.
[0072] Column gradient elution program: 0min→2min→4min→11min→13min→13.1min→14min, mobile phase A: 5%→5%...
Embodiment 6
[0083] Embodiment 6 precision test
[0084] Using the detection method provided in Example 1, take the standard solution provided in Example 1, inject 6 needles continuously, and calculate the RSD of each index component with the peak area, which are respectively scopoletin and (1S, 6R, 7R, 10R) The RSD values (n=6) of the peak areas of -6-carboxy-10-methyl-α-methylene-1-(1-oxobutyl)-cyclohexane acrylic acid were 0.46%, 2.17%, indicating that the instrument The precision is good.
Embodiment 7
[0085] The investigation of embodiment 7 linear relationship
[0086]Accurately weigh an appropriate amount of scopoletin and (1S,6R,7R,10R)-6-carboxy-10-methyl-α-methylene-1-(1-oxobutyl)-cyclohexaneacrylic acid, add Made with 50% methanol containing scopoletin 4.17μg / mL, (1S,6R,7R,10R)-6-carboxy-10-methyl-α-methylene-1-(1-oxobutyl)- The mixed standard solution of cyclohexane acrylic acid 19.50 μg / mL was used as the mother liquor and diluted step by step with 50% methanol to obtain scopoletin concentrations of 3.34 μg / mL, 1.67 μg / mL, 0.83 μg / mL, 0.42 μg / mL, 0.10 μg / mL and (1S,6R,7R,10R)-6-carboxy-10-methyl-α-methylene-1-(1-oxobutyl)-cyclohexaneacrylic acid Concentrations of 15.60 μg / mL, 7.80 μg / mL, 3.90 μg / mL, 1.95 μg / mL, 0.49 μg / mL of the standard solution, each accurately draw 1 μL, using the detection method provided in Example 1 to measure, the peak area Integral value y versus concentration x (μg·mL -1 ) for regression analysis, the linear regression equations are resp...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More