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Preparation method of peony seed polypeptides

A technology of peony seeds and peony seed meal, which is applied to the protein components of waste, protein food processing, and tissue-based treatment. It can solve the problems of long microbial fermentation time, poor combination effect, and cumbersome procedures. The effect of short time consumption and simple process

Inactive Publication Date: 2018-11-06
龙池牡丹实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing peony seed polypeptides are generally prepared by microbial fermentation and / or protease, but the effect of using protease and microbial fermentation alone is poorer than the combination of the two, and microbial fermentation takes a long time and the procedure is cumbersome. High yield requires multiple types of enzyme joint treatment, which is costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] The invention discloses a method for preparing peony seed polypeptide, which can be realized by appropriately improving process parameters for reference by those skilled in the art. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The preparation method of the present invention has been described through the examples, and the relevant personnel can obviously make changes or appropriate changes and combinations to the preparation method described herein without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention .

[0020] In the specific embodiment of the present invention, related to the comparative test, each test group has the same test environment and raw materials, except for the due differences.

[0021] The preparation method of a peony s...

Embodiment 1

[0022] Embodiment 1: preparation method of the present invention

[0023] The defatted peony seed meal with normal color, no peculiar smell, and no mildew is used as raw material; the defatted peony seed meal is crushed with a pulverizer and passed through a 40-mesh sieve to obtain peony seed meal powder, which is freeze-dried and freeze-dried. Dry powder and water are mixed evenly according to the weight ratio of 1:5, and then the peony seed meal mixture is prepared by colloidal grinding and then homogenized by a high-pressure homogenizer, and the homogenization pressure is 25-30MPa;

[0024] Adding 0.03% of the total weight of cellulase enzymolysis to the peony seed meal mixture to obtain peony seed meal enzymatic solution 1, and then adding 0.04% of its total weight Alcalase enzyme and papaya to the peony seed meal enzymatic solution 1 The mixed enzyme liquid of protease (the volume ratio of Alcalase enzyme and papain is 1:1) is enzymatically hydrolyzed to obtain peony seed...

Embodiment 2

[0026] Embodiment 2: comparative test

[0027] Contrast process 1: Same as Example 1, the difference is that without freeze-drying, the peony seed meal powder is directly mixed with water to make a peony seed meal mixture;

[0028] Contrast process 2: same as embodiment 1, the difference is that freeze-drying and cellulose enzymolysis are not carried out, and the mixed enzyme enzyme of Alcalase enzyme and papain is directly carried out with the peony seed dregs mixed solution;

[0029] Contrast process 3: with embodiment 1, difference is to adopt neutral Neutrase protease and papain during mixed enzymolysis;

[0030] Contrast process 4: with embodiment 1, difference is that adopts Alcalase enzyme and trypsin during mixed enzymolysis;

[0031] Yield = peony seed polypeptide / defatted peony seed meal * 100%

[0032] The results are shown in Table 1.

[0033] Table 1

[0034] craft

[0035] It can be seen from Table 1 that after freeze-drying, cellulose hydrolysis an...

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Abstract

The present invention relates to the technical field of health-care food and discloses a preparation method of peony seed polypeptides. The preparation method comprises the following steps: defatted peony seed meal is crushed; then the peony seed meal powder is freeze-dried; then the freeze-dried peony seed meal powder is mixed with water uniformly; the mixture is homogenized to prepare a peony seed meal mixture; then cellulase is added for enzymatic hydrolysis to prepare a peony seed meal enzymatic hydrolysate 1; then a mixed enzyme solution of Alcalase enzyme and papain is added for enzymatic hydrolysis to prepare a peony seed meal enzymatic hydrolysate 2; the peony seed meal enzymatic hydrolysate 2 is subjected to high temperature enzyme deactivation; then a solid-liquid separation is conducted to prepare a peony seed polypeptide solution; and the peony seed polypeptide solution is concentrated and dried to obtain the peony seed polypeptides. The freeze-drying technology is firstlyused to conduct wall-breaking of the peony seed meal raw materials; then the cellulase is firstly used to remove binding of proteins by other substances in the peony seed meal; and finally the appropriate mixed enzymolysis combination of the Alcalase enzyme and papain are used to conduct the high-efficiency enzymolysis to obtain the high-yield peony seed polypeptides. At the same time, the used proteases are small in number; and the whole technology is simple and short in time-consuming.

Description

technical field [0001] The invention relates to the technical field of health food, in particular to a preparation method of peony seed polypeptide. Background technique [0002] Peony is a deciduous subshrub of the genus Paeoniae, and it is one of the wild relatives of Chinese cultivated peony varieties. Peony flowers are edible, and peony seed oil is rich in unsaturated fatty acids, which has very important health care and medicinal value. The unsaturated fatty acids in peony seed oil extracted from peony seeds are mainly linolenic acid, including oleic acid, linoleic acid, and various essential trace elements such as K, Fe, Zn, etc., but there are still a lot of nutrients Remaining in the peony seed cake after extracting the oil, protein substances account for 30-40%. [0003] Peony polypeptide refers to the oligopeptide mixture obtained by separating and refining the protein in peony seed cake through indirect microbial fermentation or direct action of protease. Exist...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23J3/34A23J3/24
CPCA23J1/005A23J3/24A23J3/346
Inventor 王封
Owner 龙池牡丹实业有限公司
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