Tea variety discrimination method based on chemical components

A discriminant method and discriminant function technology, applied in scientific instruments, analytical materials, material analysis by electromagnetic means, etc., can solve the problems of lack of science, cumbersome detection process, and cumbersome operation of the six major teas, and achieve automatic detection Identify, improve accuracy and effectiveness, identify simple and fast effects

Active Publication Date: 2018-11-06
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this identification method has the following defects: first, the types of chemical components that can be selected as discriminant factors are limited, and the identification of the six major teas is not scientific, and the accuracy of identification is not high, and only some teas can be accurately identified; secondly , multi-step judgment is required, the number of discriminant formulas to be substituted is large, the operation is cumbersome, simple and effective one-step discrimination cannot be performed, and it is difficult to quickly classify tea leaves
This method needs to detect many compounds. At the same time, in order to detect the content, it is necessary to establish a standard curve of 21 compounds, and to detect the content of each tea sample. The detection process is relatively cumbersome.

Method used

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  • Tea variety discrimination method based on chemical components
  • Tea variety discrimination method based on chemical components
  • Tea variety discrimination method based on chemical components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Step 1. Sample collection and pretreatment: select six types of tea samples to be identified from the same batch of fresh leaves, each type of tea sample to be identified is 6 tea samples, and a total of 36 dry tea samples are ground into powder; For each tea sample, accurately weigh 50 mg tea sample, accurately add 800 μL of 70% methanol; vortex shake well, sonicate; Methanol solution) in the sample bottle for detection.

[0040] Step 2, select the detection platform and set the platform environment: detect the tea samples processed in step 1 by LC-MS, wherein, chromatographic conditions: use Hypersil Gold chromatographic column; mobile phase 0.1% formic acid water (phase A)-containing 0.1% formic acid in acetonitrile (phase B), gradient elution (0-2min, 5-40%B; 2-7min, 40-80%B; 7-11min, 80-95%B; 11-15min, 95% B); the column temperature is 35° C., the flow rate is 0.3 mL / min, and the injection volume is 4 μL. Mass spectrometry conditions: Q Exactive high-resolution c...

Embodiment 2

[0050] Step 1, sample collection and pretreatment: All tea samples in Example 1 are processed through the same batch of fresh leaves. In order to be closer to the actual detection and eliminate the influence caused by the same batch of fresh leaves in Example 1, the tea samples sold in the market are randomly collected. Part of the tea samples in the six teas, a total of 43 tea samples (Table 3), were processed according to the same sample pretreatment method and steps 2 and 3 in Example 1 to these 43 tea samples to be identified.

[0051] Table 3 Source information table of 43 tea samples to be identified

[0052]

[0053]

[0054]

[0055] Step 4. Discrimination of tea types: according to the number n of tea samples of each type in the tea sample library i With the method that the ratio of the number of tea samples of each type to be discriminated in the tea sample set is 2:1, 43 tea samples are randomly divided into a tea sample bank as a sample and a tea sample se...

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Abstract

An embodiment of the invention provides a tea variety identification method based on chemical components. The method comprises steps as follows: S1, an identification function of each tea variety is established; S2, the variety of to-be-discriminated tea samples is judged according to the identification function of each tea variety. According to the provided method, the tea variety is discriminated with a chemical method, the problems in sensory identification are solved, and tea is more objectively and scientifically classified, so that the accuracy and the effectiveness of judgement resultsare improved; ion strength of multiple compounds in tea is taken as an index for establishing the identification function, and the correct recognition ratio of identification is high; single tea sample can be classified or multiple tea samples can be classified in batches only through one step without manual intervention, automatic detection identification can be realized, and identification is simple and rapid.

Description

technical field [0001] The embodiments of the present invention relate to the field of food, and more specifically, to a method for discriminating tea based on chemical components. Background technique [0002] Tea tree is a perennial evergreen leaf plant belonging to the genus Camellia of the family Theaceae. Tea is made from the fresh leaves of tea tree through different processing techniques. It is rich in various functional chemical components such as catechins, flavonols and flavonol glycosides, amino acids and alkaloids. The current tea classification methods are usually based on processing methods, combined with quality characteristics and appearance differences, and divide tea into six major tea categories: green tea, yellow tea, dark tea, white tea, oolong tea, and black tea. [0003] At present, the identification of tea types is mainly based on sensory evaluation, and sensory evaluation is easily affected by factors such as the physiological conditions, work expe...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 王一君阚志鹏宁井铭章林忠胡丽珍李大祥宛晓春
Owner ANHUI AGRICULTURAL UNIVERSITY
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