Tea variety discrimination method based on chemical components
A discriminant method and discriminant function technology, applied in scientific instruments, analytical materials, material analysis by electromagnetic means, etc., can solve the problems of lack of science, cumbersome detection process, and cumbersome operation of the six major teas, and achieve automatic detection Identify, improve accuracy and effectiveness, identify simple and fast effects
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Embodiment 1
[0039] Step 1. Sample collection and pretreatment: select six types of tea samples to be identified from the same batch of fresh leaves, each type of tea sample to be identified is 6 tea samples, and a total of 36 dry tea samples are ground into powder; For each tea sample, accurately weigh 50 mg tea sample, accurately add 800 μL of 70% methanol; vortex shake well, sonicate; Methanol solution) in the sample bottle for detection.
[0040] Step 2, select the detection platform and set the platform environment: detect the tea samples processed in step 1 by LC-MS, wherein, chromatographic conditions: use Hypersil Gold chromatographic column; mobile phase 0.1% formic acid water (phase A)-containing 0.1% formic acid in acetonitrile (phase B), gradient elution (0-2min, 5-40%B; 2-7min, 40-80%B; 7-11min, 80-95%B; 11-15min, 95% B); the column temperature is 35° C., the flow rate is 0.3 mL / min, and the injection volume is 4 μL. Mass spectrometry conditions: Q Exactive high-resolution c...
Embodiment 2
[0050] Step 1, sample collection and pretreatment: All tea samples in Example 1 are processed through the same batch of fresh leaves. In order to be closer to the actual detection and eliminate the influence caused by the same batch of fresh leaves in Example 1, the tea samples sold in the market are randomly collected. Part of the tea samples in the six teas, a total of 43 tea samples (Table 3), were processed according to the same sample pretreatment method and steps 2 and 3 in Example 1 to these 43 tea samples to be identified.
[0051] Table 3 Source information table of 43 tea samples to be identified
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[0055] Step 4. Discrimination of tea types: according to the number n of tea samples of each type in the tea sample library i With the method that the ratio of the number of tea samples of each type to be discriminated in the tea sample set is 2:1, 43 tea samples are randomly divided into a tea sample bank as a sample and a tea sample se...
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