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Disease marker indirect immunofluorescence assay method based on dopamine modified ternary quantum points

A ternary quantum dot and dopamine technology, which is applied in the fields of optical and biochemical engineering, can solve the problem that the sensitivity of disease markers needs to be improved

Active Publication Date: 2018-11-06
FUJIAN MEDICAL UNIV
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Problems solved by technology

[0004] Conventional fluorescent immunoassay detects the change of fluorescent signal through the combination of quantum dots and corresponding antibodies, but the complex modification process required for the combination of quantum dots and antibodies is the key obstacle, and the sensitivity of the detection of disease markers needs to be improved

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  • Disease marker indirect immunofluorescence assay method based on dopamine modified ternary quantum points
  • Disease marker indirect immunofluorescence assay method based on dopamine modified ternary quantum points
  • Disease marker indirect immunofluorescence assay method based on dopamine modified ternary quantum points

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Embodiment Construction

[0039] The present invention is described in detail below in conjunction with accompanying drawing and embodiment:

[0040] Invention principle introduction:

[0041] Ternary Quantum Dots CuInS Based on Dopamine Functionalization 2 / ZnS (CuInS 2 / ZnS QDs-DA) reaction mechanism of indirect fluorescent immunosensing technology is shown in Figure 1. First, tyrosinase (TYR) is bound to detection antibodies for disease biomarkers via a copper-free click reaction. Additionally, the corresponding biotin-modified aptamers were bound to avidin-coated 96-well plates, and disease biomarkers and TYR-detection antibodies were sequentially added. Add CuInS after washing away excess TYR-detection antibody 2 / ZnS QDs-DA. TYR Catalyzes the Oxidation of CuInS in the Presence of Disease Biomarkers 2 The dopamine moiety in / ZnS QDs-DA converts it into dopamine quinone leading to fluorescence quenching. Catalytic oxidation of CuInS by detecting TYR 2 / ZnS QDs-DA reaction-induced electron t...

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Abstract

The invention discloses a disease marker indirect immunofluorescence assay method based on dopamine modified ternary quantum points. According to the method, firstly, tyrosinase (TYR) is combined withdetection antibodies of disease biomarkers through copper-free click reaction; in addition, biotin modified corresponding aptamers are combined with avidin coated 96 hole plates; the disease biomarkers and TYR-detection antibodies are sequentially added; after washing excessive TYR-detection antibodies, CuInS2 / ZnS QDs-DA is added; under the existence of the disease biomarkers, the TYR can realizethe catalytic oxidation on dopamine parts in CuInS2 / ZnS QDs-DA so that the dopamine parts can be converted into dopamine quinone to cause fluorescence quenching. Through detecting the change of fluorescence signals due to electron transferring caused by reaction of CuInS2 / ZnS QDs-DA catalytic oxidation reaction by TYR, the high-sensitivity detection on the disease biomarkers can be realized.

Description

technical field [0001] The invention relates to the fields of optical and biochemical engineering, in particular to a dopamine-based ternary quantum dot CuInS 2 Construction and application of indirect fluorescent immunosensing technology for detection of disease biomarkers using ZnS. Background technique [0002] Disease biomarkers refer to biochemical indicators that can mark changes or possible changes in system, organ, tissue, cell and subcellular structure or function due to disease, and can be used as disease diagnosis, differential diagnosis, curative effect evaluation, disease staging, Auxiliary indicators for disease risk assessment, prognosis estimation, and health evaluation. In recent years, routine detection methods for disease biomarkers mainly include instrumental analysis and enzyme-linked immunosorbent assay (ELISA). Among them, the instrumental analysis method cannot be widely used due to the disadvantages of expensive equipment and complicated operation....

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 韩志钟陈丽陈敬华李春艳
Owner FUJIAN MEDICAL UNIV
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