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A kind of production method of cane juice beverage rich in fructooligosaccharide

The technology of a fructooligosaccharide and a production method, which is applied in the production field of fructooligosaccharide-rich cane juice beverages, can solve the problems of high price, high production cost, difficult separation and the like, and achieves the realization of continuous reaction, simplified purification process and easy automation. production effect

Inactive Publication Date: 2021-10-22
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Fructosyltransferase can catalyze the conversion of sucrose into fructooligosaccharides, but the enzyme is easily soluble in water, expensive, difficult to separate from the substrate after production, and can only be used once, resulting in high production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of production method that is rich in fructooligosaccharide sucrose juice beverage, comprises the steps:

[0028] (1) Dissolve the mixture with a molar ratio of Al: citric acid = 1:2 in a small amount of distilled water, stir until a clear viscous liquid, and slowly heat the solution in a water bath at 80°C for 4 hours to evaporate excess water and then heat it at 90°C Heating at constant temperature for 12 h. The obtained gel was dried at 120 °C for 12 h, and then calcined at 500 °C to obtain nano-Al 2 o 3 , denoted as n-Al 2 o 3 .

[0029] (2) Add wt%=1% nickel nitrate solution to n-Al 2 o 3 In the middle, let it stand for 24h, dry, and bake at 500°C to get Ni-loaded nano-Al 2 o 3 , denoted as n-Al 2 o 3 -1Ni.

[0030] (3) Dissolve the fructosyltransferase in the buffer solution of citric acid and sodium citrate with pH=6, prepare the enzyme solution with a concentration of 10 mg / mL, and weigh 10 mg nanometer n-Al 2 o 3 -1Ni alumina carrier, add 10m...

Embodiment 2

[0034] A kind of production method that is rich in fructooligosaccharide sucrose juice beverage, comprises the steps:

[0035] (1) Dissolve the mixture with a molar ratio of Al: citric acid = 1:2 in a small amount of distilled water, stir until a clear viscous liquid, and slowly heat the solution in a water bath at 80°C for 4 hours to evaporate excess water and then heat it at 90°C Heating at constant temperature for 12 h. The obtained gel was dried at 120 °C for 12 h, and then calcined at 500 °C to obtain nano-Al 2 o 3 , denoted as n-Al 2 o 3 .

[0036] (2) Add wt%=5% nickel nitrate solution to n-Al 2 o 3 In the middle, let it stand for 24h, dry, and bake at 500°C to get Ni-loaded nano-Al 2 o 3 , denoted as n-Al 2 o 3 -5Ni.

[0037](3) Dissolve the fructosyltransferase in the buffer solution of citric acid and sodium citrate with pH=6, prepare the enzyme solution with a concentration of 10 mg / mL, and weigh 10 mg nanometer n-Al 2 o 3 -5Ni alumina carrier, add 10mL...

Embodiment 3

[0041] A kind of production method that is rich in fructooligosaccharide sucrose juice beverage, comprises the steps:

[0042] (1) Dissolve the mixture with a molar ratio of Al: citric acid = 1:2 in a small amount of distilled water, stir until a clear viscous liquid, and slowly heat the solution in a water bath at 80°C for 4 hours to evaporate excess water and then heat it at 90°C Heating at constant temperature for 12 h. The obtained gel was dried at 120 °C for 12 h, and then calcined at 500 °C to obtain nano-Al 2 o 3 , denoted as n-Al 2 o 3 .

[0043] (2) Add wt%=1% nickel nitrate solution to n-Al 2 o 3 In the middle, let it stand for 24h, dry, and bake at 500°C to get Ni-loaded nano-Al 2 o 3 , denoted as n-Al 2 o 3 -1Ni.

[0044] (3) Dissolve the fructosyltransferase in the buffer solution of citric acid and sodium citrate with pH=6, prepare the enzyme solution with a concentration of 10 mg / mL, and weigh 10 mg nanometer n-Al 2 o 3 -1Ni alumina carrier, add 10m...

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PUM

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Abstract

The invention discloses a production method of cane juice beverage rich in fructo-oligosaccharides. Glycosyltransferase is immobilized on a water-insoluble nano-oxide carrier, so that the immobilized enzyme is easily separated from a substrate and a product, and then The immobilized fructosyltransferase converts the sucrose in the sugarcane juice into fructooligosaccharides, reduces the sugar content of the sugarcane juice, improves the health-preserving effect of the sugarcane juice, and increases the value of the sugarcane juice.

Description

technical field [0001] The invention relates to the technical field of beverage preparation, in particular to a production method of fructooligosaccharide-rich cane juice beverage. Background technique [0002] Sugarcane juice contains a large amount of iron, zinc, manganese and other essential trace elements, among which the content of iron is particularly high, reaching 9mg / kg, ranking first among fruits and known as "blood-enriching fruit". In addition, sugarcane juice also contains glutamic acid, aspartic acid, serine and other amino acids, as well as organic acids such as malic acid and citric acid. Sugarcane fresh juice has the characteristics of rich nutrition, delicious taste, laxative and decongestion, and alleviating alcoholism. However, sugarcane juice contains a lot of sucrose, fructose and glucose, of which the sucrose content is 12%-18%. A large intake of these sugars can easily lead to some health problems, such as tooth decay, obesity and other problems. Lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L2/02A23L2/52A23L2/60A23L2/84A23L33/10A23L33/105
CPCA23L2/02A23L2/52A23L2/60A23L2/84A23V2002/00A23L33/10A23L33/105A23V2200/30A23V2250/28
Inventor 王璐陈杰博雷玉凤
Owner FUJIAN AGRI & FORESTRY UNIV
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