The invention belongs to the technical field of production of alcohols, and relates to a preparation method of a sea cucumber wine, which comprises the steps of raw material treatment, water immersion of sea cucumber, rice steaming with sea cucumber, fermentation and preparation of finished product. The method specifically comprises the following steps: processing a grain raw material into polished rice, mixing sea cucumber powder with the polished rice, immersing in a grape juice water solution, steaming the sea-cucumber / rice-immersed water, putting into a fermentation tank, adding malt and yeast, adding jam, evenly mixing with water, sinking the tank for 10-12 hours while controlling the temperature at 24-26 DEG C, heating, fermenting for 3-5 days while controlling the temperature at 30-31 DEG C, transferring the wine mash into another fermentation tank, standing to ferment at 15-18 DEG C for 20-30 days, filtering the fermented wine rice with a filtering machine, standing at 0-5 DEG C for 1-2 days, taking the supernate, sealing and sterilizing. The method has the advantages of simple preparation technique and accessible equipment, has the flavor of Molao wine and the nutrition of the sea cucumber, enhances the health-care and health-preserving effects of the product, and has unique mouthfeel.