Chemiluminiscent microfluidic chip based on magnetic beads

Abstract

The invention discloses a chemiluminiscent microfluidic chip based on magnetic beads. The chemiluminiscent microfluidic chip comprises a chip body, wherein the chip body is provided with a sample cavity, a reaction cavity and a waste liquor cavity; the sample cavity is communicated with the reaction cavity through a sample feed pipe; the inner wall of the reaction cavity is arc-shaped, and a magnet enriching area for separating the magnetic beads through strong magnets is arranged on the inner wall of the reaction cavity. Therefore, for the microfluidic chip, the magnetic beads are collected and fixed during magnetic separation cleaning and can be stored, effective recovery can be realized, and loss of the magnetic beads due to circulating flushing of a cleaning solution is avoided.

Description

technical field [0001] The invention discloses a chemiluminescent microfluidic chip based on magnetic beads. Background technique [0002] With the rapid development of precision medicine and hierarchical diagnosis and treatment, in vitro diagnostic reagents are increasingly demanding higher requirements for the two important indicators of detection repeatability and portability. At this stage, small POCT rapid diagnostic equipment can only achieve qualitative or semi-quantitative test results, and the detection reagents have low sensitivity and poor repeatability, while large-scale chemiluminescence equipment is expensive, and small hospitals have limited funds and small sample sizes. It is not suitable to be equipped with large-scale testing instruments, and only relying on large hospitals cannot fully cover all audiences. Therefore, the development of in vitro diagnostic reagents with simple operation, high sensitivity, good repeatability and short detection time has ver...

AI Technical Summary

Problems solved by technology

[0010] 3. It is difficult to control the magnetic bead reagent

[0011] The magnetic beads used in the chemiluminescent reagents are prone to magnetic loss in the internal structure of the chip, resulting in the loss of a part of the immune complex along with the small particles of the magnetic beads, resulting in large fluctuations in the measured value of the final reaction result

Part of the problem of magnetic loss is due to the difficulty of dissolving the magnetic beads during the reaction process, and the other part is due to the structure of the chip that causes the magnetic beads to be separated incompletely during magnetic separation, so they are washed away

Images