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Preparation method of fungal Candida biofilm flow model

A biofilm and flow model technology, applied in the biological field, can solve the problems of complex operation, rarely mixed biofilm incubation, and low flux.

Pending Publication Date: 2018-11-13
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the currently commonly used biofilm flow models are relatively complicated to operate, and the flux is relatively small, and they are rarely used for the incubation of mixed biofilms.

Method used

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  • Preparation method of fungal Candida biofilm flow model
  • Preparation method of fungal Candida biofilm flow model
  • Preparation method of fungal Candida biofilm flow model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one: a kind of preparation method of Candida fungus biofilm flow model

[0030] A method for preparing a fungal biofilm flow model, comprising construction of a fungal biofilm flow model device, fungal strain cultivation, fungal biofilm incubation, fungal biofilm evaluation, and scanning electron microscope detection. The specific plan is as follows.

[0031] (1) Construction of fungal biofilm flow model device

[0032] The fungal biofilm flow model device includes a beaker containing culture solution, a digital display peristaltic pump, a liquid separator (50ml) made of centrifuge tubes, and four centrifuge tubes (50ml / pc) for biofilm incubation and its stand, a beaker for the effluent.

[0033] The preparation method of the liquid separator injector is as follows: respectively punch one and a row of four 2mm holes at the relative positions of the centrifuge tube wall, when placed, one hole directly above is a liquid inlet hole, and 4 The first hole is th...

Embodiment 2

[0054] Embodiment two: a kind of preparation method of candida fungus biofilm flow model

[0055] The difference from Example 1 is that the strains are Candida albicans SC 5314 and Candida glabrataATCC64677. Candida albicans SC 5314 was donated by the School of Pharmacy, Shanghai Second Military Medical University, and Candidaglabrata ATCC64677 was provided by the First Affiliated Hospital of Anhui Medical University.

[0056] like Figure 6 As shown, under the flow regime, the stability of the flow model device after four biofilm incubation tubes were incubated for 24h with Candidaalbicans SC 5314-Candida glabrata ATCC64677 mixed biofilm was tested by XTT method. We found that the OD values ​​(492nm) of the dissociated bacterial cells on the catheter sheet in the four incubation tubes were 0.9633±0.1829, 0.9827±0.0460, 0.9223±0.0801, 0.9710±0.0370, and there was no significant difference between them.

[0057] like Figure 7 As shown, under the flow state, the stability of...

Embodiment 3

[0060] Embodiment three: a kind of preparation method of Candida fungus biofilm flow model

[0061] A preparation method of a Candida fungus biofilm flow model, comprising a Candida fungus biofilm flow model device, such as figure 1 As shown, the Candida fungus biofilm flow model device includes a first container 1 for containing fungal culture medium, a peristaltic pump 2, a separator 3, and four second containers for biofilm incubation. Container 4 and a third container 5 that accommodates the effluent; the liquid separator is provided with a liquid inlet, and four outlet holes are provided below the liquid inlet; the top of the second container The top hole is provided, and the bottom hole is provided with the bottom hole; the four second containers for biofilm incubation are located on the same level, and the liquid inlet hole of the liquid separator is connected with the outlet of the peristaltic pump through the pipeline. The liquid port is connected, the liquid inlet o...

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Abstract

The invention discloses a preparation method of a fungal Candida biofilm flow model. The preparation method comprises the following steps: establishing a fungal biofilm flow model device, culturing afungal culture, incubating a fungal biofilm, evaluating the fungal biofilm and performing scanning electron microscopy. The preparation method of the fungal Candida biofilm flow model has the advantages of easiness in operation and high stability and reproducibility.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a Candida fungus biofilm flow model. Background technique [0002] Fungal biofilm refers to the membrane-like multicellular complex formed by fungi attaching to the host cavity or the surface of biological materials, secreting polysaccharide matrix, fibrin, lipoprotein, etc., and surrounding themselves in it. Many studies have found that the degree of drug resistance of various fungi is related to the process of biofilm formation, and the more mature the biofilm, the stronger the drug resistance of the fungi. [0003] Fungi may form biofilms both in vivo and in vitro. There are two main forms of in vitro biofilm formation: static and dynamic. The biofilm formed under dynamic conditions has more abundant extracellular matrix and more complex structure, which makes the filmed fungus have stronger drug resistance. [0004] In vitro biofilm models in...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N1/14C12R1/645
CPCC12M23/58C12N1/14
Inventor 邵菁汪天明施高翔段强军陆克乔李倩倩吴大强汪长中
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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