Rare cell capture system and application thereof
A capture chamber and capture device technology, used in large-capacity systems for separating rare cells, systems for collecting circulating tumor cells from blood with low hemolysis, and in the online field, which can solve the problem of inability to complete whole-body blood screening analysis and blood collection samples Problems such as small amount and sample selectivity bias
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0102] Example 1: Detection of capture ability of breast cancer circulating tumor cells
[0103] 1. Streptavidin-bottomed bread quilt
[0104] 1.1 Wash the chip 3 times with 200 μL sterile PBS to make sure that there are no air bubbles left in the chip channel.
[0105] Inject 100ul of streptavidin protein into the chip with a syringe, then place the chip in a wet box, and coat at 37 degrees Celsius for 2 hours.
[0106] 2. Coating of capture antibody
[0107] 2.1 Add human-EpCAM capture antibody (biotin-labeled) to 1×PBS sterile solution, mix well, and prepare antibody capture working solution with a concentration of 5 μg / ml.
[0108] 2.2 Inject 100 μL of antibody capture working solution into the chip with a syringe, then place the chip in a wet box and incubate overnight at 4°C. During the entire incubation process, ensure that all chip flow channels are covered with antibody capture working solution.
[0109] 2.3 Wash the chip coated with the capture antibody with 200 ...
Embodiment 2
[0121] Example 2: Detection of Capture Efficiency of Breast Cancer Cells
[0122] 1. Streptavidin-bottomed bread quilt
[0123] 1.1 Wash the chip 3 times with 200 μL sterile PBS to make sure that there are no air bubbles left in the chip channel.
[0124] Inject 100ul of streptavidin protein into the chip with a syringe, then place the chip in a wet box, and coat at 37 degrees Celsius for 2 hours.
[0125] 2. Coating of capture antibody
[0126] 2.1 Add human-EpCAM capture antibody (biotin-labeled) to 1×PBS sterile solution, mix well, and prepare antibody capture working solution with a concentration of 5 μg / ml.
[0127] 2.2 Inject 100 μL of antibody capture working solution into the chip with a syringe, then place the chip in a wet box and incubate overnight at 4°C. During the entire incubation process, ensure that all chip flow channels are covered with antibody capture working solution.
[0128] 2.3 Wash the chip coated with the capture antibody with 200 μL of 1×PBS ste...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| height | aaaaa | aaaaa |
| height | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More