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Rare cell capture system and its application

A capture chamber and capture device technology, applied in a large-capacity, low-hemolytic system for collecting circulating tumor cells from blood, a system for separating rare cells, and in the online field, it can solve the problem of inability to screen and analyze whole-body blood, cells in blood, etc. Damage, sample selectivity bias, etc.

Active Publication Date: 2021-01-01
CHENGDU PRECISOME BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no matter what kind of in vitro CTCs collection and separation device is available, it is not possible to screen and analyze whole body blood. There are problems such as small blood sample volume, insufficient number of CTCs collection, and possible selective bias of samples.
Connecting the existing external CTCs collection and separation device online to the blood circulation in the body may cause damage to the cells in the blood, and there is a risk of hemolysis. The blood after hemolysis caused by the device will produce cell fragments to promote coagulation and reduce blood quality, thereby May cause potential clinical risk

Method used

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  • Rare cell capture system and its application
  • Rare cell capture system and its application
  • Rare cell capture system and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1: Detection of capture ability of breast cancer circulating tumor cells

[0103] 1. Streptavidin-bottomed bread quilt

[0104] 1.1 Wash the chip 3 times with 200 μL sterile PBS to make sure that there are no air bubbles left in the chip channel.

[0105] Inject 100ul of streptavidin protein into the chip with a syringe, then place the chip in a wet box, and coat at 37 degrees Celsius for 2 hours.

[0106] 2. Coating of capture antibody

[0107] 2.1 Add human-EpCAM capture antibody (biotin-labeled) to 1×PBS sterile solution, mix well, and prepare antibody capture working solution with a concentration of 5 μg / ml.

[0108] 2.2 Inject 100 μL of antibody capture working solution into the chip with a syringe, then place the chip in a wet box and incubate overnight at 4°C. During the entire incubation process, ensure that all chip flow channels are covered with antibody capture working solution.

[0109] 2.3 Wash the chip coated with the capture antibody with 200 ...

Embodiment 2

[0121] Example 2: Detection of Capture Efficiency of Breast Cancer Cells

[0122] 1. Streptavidin-bottomed bread quilt

[0123] 1.1 Wash the chip 3 times with 200 μL sterile PBS to make sure that there are no air bubbles left in the chip channel.

[0124] Inject 100ul of streptavidin protein into the chip with a syringe, then place the chip in a wet box, and coat at 37 degrees Celsius for 2 hours.

[0125] 2. Coating of capture antibody

[0126] 2.1 Add human-EpCAM capture antibody (biotin-labeled) to 1×PBS sterile solution, mix well, and prepare antibody capture working solution with a concentration of 5 μg / ml.

[0127] 2.2 Inject 100 μL of antibody capture working solution into the chip with a syringe, then place the chip in a wet box and incubate overnight at 4°C. During the entire incubation process, ensure that all chip flow channels are covered with antibody capture working solution.

[0128] 2.3 Wash the chip coated with the capture antibody with 200 μL of 1×PBS ste...

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Abstract

The present invention relates to the detection of biological and pathological body fluids, in particular to a system for separating rare components from biological fluid samples and its application, further to a system for separating rare cells from whole blood cell populations and its application, in particular to a High-capacity, on-line, low-hemolytic collection of circulating tumor cells from blood and its application.

Description

technical field [0001] The present invention relates to the detection of biological and pathological body fluids, in particular to a system for separating rare components from biological fluid samples and its application, further to a system for separating rare cells from whole blood cell populations and its application, in particular to a High-capacity, on-line, low-hemolytic collection of circulating tumor cells from blood and its application. Background technique [0002] Rare cells refer to some atypical cells in biological fluid samples (including blood, pleural effusion, ascites, urine, cerebrospinal fluid, etc.). Studies have shown that the collection of rare cells and the use of them to complete NGS analysis have important guiding significance for finding potential therapeutic mechanisms, pathological mechanisms and targeted drug development of diseases. At present, the research methods for the detection of rare cells in blood mainly include flow cytometry, morpholo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N5/09
CPCC12M47/04C12N5/0693C12N2509/00A61M1/3679A61M2205/0244
Inventor 石虎兵李燕雏徐琪孔祥菊王学焱韩赫文
Owner CHENGDU PRECISOME BIOTECH CO LTD
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