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Method for recovering fish protein from canned sardine processing fish soup waste liquid and application thereof

A technology of canned sardines and fish protein, applied in the field of food processing, can solve the problems of increasing the economic cost of sewage treatment, environmental pollution, etc., and achieve the effects of correcting malnutrition, removing sunburn, and reducing facial wrinkles

Inactive Publication Date: 2018-11-20
QUANZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production of canned sardines, the fish soup from boiled sardines is usually discarded as waste liquid, which will not only increase the economic cost of sewage treatment for enterprises, but also cause great pollution to the environment

Method used

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  • Method for recovering fish protein from canned sardine processing fish soup waste liquid and application thereof
  • Method for recovering fish protein from canned sardine processing fish soup waste liquid and application thereof
  • Method for recovering fish protein from canned sardine processing fish soup waste liquid and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] A method for recovering fish protein in canned sardine processing fish broth waste liquid, comprising the following steps:

[0020] (1) sardine canned fish soup waste liquid is extracted twice with n-hexane of 2 times the mass for degreasing, the extracted upper layer solution is recovered with n-hexane, and the extracted lower layer aqueous solution is collected for use;

[0021] (2) Put the lower aqueous solution into test tubes, adjust the pH value of the lower aqueous solution to 4.0 with 0.1mol / L HCl solution, let it stand for 25 minutes, then centrifuge at 7000r / min at 4°C for 20 minutes, discard the upper solution, and collect the precipitate ;

[0022] (3) Take out the precipitate and place it in a petri dish, place it in a freeze dryer, and carry out low-temperature and reduced-pressure drying at a temperature of -78° C. and a vacuum degree of <5 Pa to obtain fish protein.

Embodiment 2

[0024] A method for recovering fish protein in canned sardine processing fish broth waste liquid, comprising the following steps:

[0025] (1) sardine canned fish soup waste liquid is extracted twice with n-hexane of 2.5 times the mass for degreasing, and n-hexane is recovered from the extracted upper layer solution, and the extracted lower layer aqueous solution is collected for use;

[0026] (2) Put the lower aqueous solution into test tubes, adjust the pH value of the lower aqueous solution to 4.5 with 0.15mol / L HCl solution, let it stand for 20 minutes, then centrifuge at 4°C and 6500r / min for 25 minutes, discard the upper solution, and collect the precipitate ;

[0027] (3) Take out the precipitate and place it in a petri dish, place it in a freeze dryer, and carry out low-temperature and reduced-pressure drying at a temperature of -78° C. and a vacuum degree of <5 Pa to obtain fish protein.

Embodiment 3

[0029] A method for recovering fish protein in canned sardine processing fish broth waste liquid, comprising the following steps:

[0030] (1) sardine canned fish soup waste liquid is extracted twice with n-hexane of 2.2 times the mass for degreasing, and n-hexane is recovered from the extracted upper layer solution, and the extracted lower layer aqueous solution is collected for use;

[0031] (2) Put the lower aqueous solution into test tubes, adjust the pH value of the lower aqueous solution to 3.5 with 0.12mol / L HCl solution, let it stand for 30min, then centrifuge at 7500r / min at 4°C for 20min, discard the upper solution, and collect the precipitate ;

[0032] (3) Take out the precipitate and place it in a petri dish, place it in a freeze dryer, and carry out low-temperature and reduced-pressure drying at a temperature of -78° C. and a vacuum degree of <5 Pa to obtain fish protein.

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Abstract

The invention provides a method for recovering fish protein from canned sardine processing fish soup waste liquid and an application thereof, and belongs to the technical field of food. The canned sardine processing fish soup waste liquid is degreased with n-hexane, hydrochloric acid is added to the degreased fish soup to adjust the pH to produce a precipitate, and then the precipitate is centrifuged, separated and dried to obtain the fish protein. The fish protein in the fish soup waste liquid is obtained by salting-out, so the method has the advantages of simple technology and easy industrial production. The obtained fish protein can be separately processed into fish protein powder, or added as a nutrient to food such as biscuits, bread, cakes and seasonings to enhance food nutrition andflavor. The method can greatly improve the utilization rate of resources, reduce environmental pollution, improve the added value of fish soup waste and increase the profit of an enterprise.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for recovering fish protein in fish soup waste liquid from canned sardine processing and an application thereof. Background technique [0002] The development of my country's aquatic product processing industry is rapid year by year, and the output of marine fish processing such as hairtail, small yellow croaker, and sardine is increasing day by day. According to relevant research, the total output of aquatic products in my country in 2014 reached 6.461x 10 7 tons, of which the total fish production reached 2.711x10 7 tons, accounting for 33.33% of the world's total fish production, and ranked first in the world for 22 consecutive years, but the processing rate of aquatic products is only 35.1%. In marine fish processing waste, the content of protein, fish oil, trace elements, etc. is high, and the price is low. It is a kind of high-quality protein ...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30A23L33/17
CPCA23V2002/00A23L33/17C07K1/145C07K1/30A23V2200/32A23V2200/318A23V2250/543
Inventor 郑宗平叶南团郑瑞生郭凤仙林娈陈文静章超桦
Owner QUANZHOU NORMAL UNIV
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