Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

circRNA_26852 and application thereof

A technology of reagents and pigskin, applied in the field of circRNA_26852 and its application in pig molecular assisted breeding, can solve unclear problems

Active Publication Date: 2018-11-20
INST OF ANIMAL SCI CAAS
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether circRNA is involved in fat deposition and metabolism is still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • circRNA_26852 and application thereof
  • circRNA_26852 and application thereof
  • circRNA_26852 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1c

[0042] Example 1 circRNA sequencing and selection of differentially expressed circRNAs

[0043] 1.1 Experimental animals:

[0044]In this experiment, large white pigs and Laiwu pigs with obvious differences in fat deposition were used as materials. They were raised in Laiwu Daqian Agriculture and Animal Husbandry Co., Ltd., grown and fattened under the same feeding conditions and environment, and fed according to the nutritional requirements standard (National Research Council, NRC, 1998) To feed the diet, choose 3 large white pigs (about 100kg) and 3 Laiwu pigs (about 35kg) each with a similar weight in the species, which are 180 days old, healthy and in good condition.

[0045] 1.2 Sample collection:

[0046] After slaughtering the experimental pigs, the subcutaneous adipose tissue was quickly separated. To reduce RNA degradation, all processes were performed on ice. Afterwards, the tissue was cut into small pieces with sterile scissors, put into 5mL cryopreservation tube...

Embodiment 2

[0071] Example 2 In-depth analysis of circRNA

[0072] 2.1 Functional analysis of differentially expressed circRNA:

[0073] After obtaining the differentially expressed circRNAs, we used the information of the circRNA source genes to perform GO enrichment analysis on the differentially expressed circRNAs. The number of differential circRNAs included in each GO entry was counted, and the significance of differential circRNA enrichment in each GO entry was calculated using the hypergeometric distribution test method. Using the KEGG database to perform Pathway analysis of differential circRNA source genes, Pathway entries enriched for differential circRNA source genes can be found, and potential differential circRNAs in different samples may be related to changes in cellular pathways.

[0074] 2.2 circRNA-miRNA-mRNA interaction network diagram

[0075] The circRNA_26852 down-regulated in Laiwu subcutaneous adipose tissue was selected for subsequent analysis, combined with bioi...

Embodiment 3

[0078] Embodiment 3 real-time fluorescent quantitative PCR verification

[0079]Select circRNA_26852, 2 miRNAs (ssc-miR-486, ssc-miR-874) in the target gene of circRNA_26852 and related genes SCD, SMAD7, ABHD5, each setting 20 biological replicates (Large White pig and Laiwu pig each 20 Example), using glyceraldehyde-3-phosphate dehydrogenase (glyceraldehyde-3-phosphate dehydrogenase, GAPDH) gene and 5S rRNA as internal references, respectively, qRT-PCR method was used to verify the expression level of the gene.

[0080] 3.1 Primer design:

[0081] circRNA_26852 amplification:

[0082] Forward primer: 5'-CACCCTGTGAGAGCCAGATTTA-3' (SEQ ID NO.2)

[0083] Reverse primer: 5'-CTGAAGCGCTGTAGTACCTGG-3' (SEQ ID NO.3)

[0084] SCD amplification:

[0085] Forward primer: 5'-CCTTTATTCTTCTCTAACCCGT-3' (SEQ ID NO.4)

[0086] Reverse primer: 5'-TCAGTGAGCAGAGAGACTTGTG-3' (SEQ ID NO.5)

[0087] ABHD5 amplification:

[0088] Forward primer: 5'-GAGGAGATGGACTCCACG-3' (SEQ ID NO.6)

[008...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to circRNA_26852 and application thereof and in particular relates to circRNA_26852 and application thereof in pig molecule auxiliary breeding. Expression of circRNA in subcutaneous fat tissue of large white pigs and Laiwu pigs is identified and analyzed for a first time, hoping to find potential circRNA related to adipogenic differentiation and lipid metabolism. Results showthat with the combination of circRNA_26852 and miRNA such as ssc-miR-486 and ssc-miR-874, expression of related genes in related signal paths of fat deposition and lipid metabolism can be regulated and controlled. With the combination of circRNA_26852 and miRNA, expression of related genes in signal paths is regulated and controlled, a potential regulation and control function can be brought intoplay in fat deposition and lipid metabolism, and a base can be made for development of improved varieties of livestock.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to circRNA_26852 and its application, more specifically to circRNA_26852 and its application in pig molecular assisted breeding. Background technique [0002] As a special non-coding RNA, circRNA is ubiquitous in animal and plant tissues and cells. It is abundant, stable, conserved and tissue-specific. It has become a new research of RNA family after miRNA and lncRNA hotspot. circRNA is a kind of circular endogenous molecule produced by special alternative cleavage, which is a covalently closed circular structure, without 5' and 3' polarity, and cannot be degraded by ribonuclease. Studies have confirmed that circRNA plays an important role in the occurrence and development of diseases. Some circRNAs play an important role in the regulation of gene expression, some circRNAs regulate RNA-binding proteins, some circRNAs can be used as translation templates, and some circRNAs can ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12Q1/6888
CPCC12N15/113C12N2310/532C12Q1/6888C12Q2600/178
Inventor 苗向阳李嫒解领丽黄万龙张秀秀
Owner INST OF ANIMAL SCI CAAS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com