A molecular marker of cervical cancer and its application

A cervical cancer technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of high detection accuracy, fast and accurate quantification, and good application prospects

Active Publication Date: 2019-09-24
上海市长宁区妇幼保健院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research showing whether hsa_circ_0020594 is related to cervical cancer. The inventors further analyzed the hsa-let-7c-5p interacting with hsa_circ_0020594 and the target gene of hsa-let-7c-5p, and constructed the hsa_circ_0020594 biological information regulation network

Method used

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  • A molecular marker of cervical cancer and its application
  • A molecular marker of cervical cancer and its application
  • A molecular marker of cervical cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Data Screening and Integrated Analysis

[0037] The GEO (Gene Expression Omnibus) database is developed and maintained by NCBI (National Center for Biotechnology Information), and the GEO database is the largest database of gene expression data. Develop dataset inclusion criteria:

[0038] ①The selected data set must be genome-wide mRNA / miRNA / circRNA transcriptome data;

[0039] ② These data come from tissues of cervical squamous cell carcinoma (CSCC) case group and control group (N) (without drug stimulation or transfection);

[0040] ③ This study considers standardized or original data sets.

[0041] After screening, the following data sets were obtained:

[0042] Table 1 List of datasets included in screening

[0043]

[0044] data analysis:

[0045] The tool used was R-3.3.3, and the metaMA package was used for analysis. The method used for p-value merging in meta-analysis was the inversenormal method.

[0046] Screening criteria for differentiall...

Embodiment 2

[0051] Example 2 RT-PCR verification of the relative expression of hsa_circ_0020594 in cervical squamous cell carcinoma tissue and adjacent tissue

[0052] 1. Materials

[0053] Tissue samples from 27 patients with cervical squamous cell carcinoma who underwent surgery in the gynecological ward of the hospital were collected, and the corresponding paracancerous tissues of the patients were used as the control group. The pathological diagnosis of the patients was verified by pathologists. None of the patients had received radiotherapy or chemotherapy before operation, and had no other surgical, endocrine, metabolic, infectious diseases and other gynecological diseases such as uterus and ovary. Fresh tissue specimens were obtained under sterile conditions, washed with normal saline to remove blood, and divided into RNase-free cryopreservation tubes, quick-frozen in liquid nitrogen overnight, and stored in a -80°C refrigerator for a long time.

[0054] 2. Experimental method

...

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PUM

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Abstract

The invention relates to a cervical cancer molecular marker and an application thereof, and particularly relates to an application of hsa_circ_0020594 and a related gene hsa-let-7c-5p thereof in diagnosing cervical cancer. The inventor further excavates, integrates and analyzes the sequencing data of the cervical cancer, finds out the key molecule hsa_circ_0020594 participating in the regulation and control of the cervical cancer and the related gene has-let-7c-5p, draws a biological information regulation network, thereby providing a new molecular diagnostic marker for the diagnosis of the cervical squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the technical field of tumor gene detection, and specifically relates to cervical cancer-related circRNA molecular markers and their application in the preparation of diagnostic reagents, more specifically to hsa_circ_0020594 and its related gene hsa-let-7c-5p in the diagnosis of cervical cancer Applications. Background technique [0002] Cervical cancer is one of the three major female reproductive system malignancies, which seriously threatens women's health. Early clinical symptoms are not obvious, and the main method of diagnosis - pathological tissue biopsy is invasive. Currently, reliable molecular markers for cervical cancer include DNA, RNA, and protein. In recent years, scholars have found that more and more RNA molecular markers are abnormally expressed in cervical cancer, which are closely related to the clinicopathological features, metastasis and invasion of cervical cancer. LncRNAs, miRNAs, and circRNAs do not ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
Inventor 马成斌刘平杨龙涛张文缨吴琼蔚刘彧
Owner 上海市长宁区妇幼保健院
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