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Test strip for detecting amphetamine and preparation method and application of test strip

A technology of test strips and amphetamine, applied in the field of amphetamine detection, can solve the problems of inability to realize rapid detection of large quantities of samples, time-consuming, complicated operation, etc., and achieve the effects of short detection time, simple storage and simple operation

Inactive Publication Date: 2018-11-20
郑州左安检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the complex operation and time-consuming characteristics of the existing methods for detecting amphetamine, and can not realize the characteristics of rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, fast detection speed, Low-cost test strip for detecting amphetamine and its preparation method and application method, so as to realize rapid detection and on-site monitoring of large quantities of amphetamine samples

Method used

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  • Test strip for detecting amphetamine and preparation method and application of test strip
  • Test strip for detecting amphetamine and preparation method and application of test strip
  • Test strip for detecting amphetamine and preparation method and application of test strip

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preparation example Construction

[0041] The preparation method of the test strip for detecting amphetamine mainly comprises the following steps:

[0042] 1) Label the amphetamine antibody with fluorescent microspheres and spray it on the conjugate release pad to prepare the conjugate release pad sprayed with fluorescent microsphere-labeled amphetamine antibody;

[0043] 2) Spray the hapten-carrier protein conjugate and goat anti-mouse secondary antibody on the reaction membrane as the test line (T) and quality control line (C) to prepare the amphetamine-coated hapten-carrier protein conjugate The detection line of the substance and the reaction membrane of the quality control line coated with goat anti-mouse secondary antibody;

[0044] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the bottom plate into a test strip.

[0045] The following step-by-step detailed description:

[0046] 1. Preparation of fluorescent mi...

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Abstract

The invention discloses a test strip for detecting amphetamine and a preparation method and application of the test strip. The test strip comprises a sample absorption pad, a conjugate release pad, areaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of amphetamine can be achieved. The invention further provides a method for detecting amphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale amphetamine samples can be achieved.

Description

technical field [0001] The invention relates to the detection of amphetamine, in particular to a test strip for quantitative detection of amphetamine by using time-resolved fluorescent microsphere immunochromatography technology and a preparation method and an application method thereof. Background technique [0002] Amphetamine, or amphetamine, is the prototype of a series of synthetic drugs with pronounced stimulant effects on the central nervous system. It is classified as a drug because of the addictive nature of intravenous injection. Adverse reactions caused by amphetamine include symptoms such as excessive excitement, restlessness, insomnia, tremor, tension and irritability. Physical tolerance to amphetamines develops very quickly, so long-term users must take more and more. The most severe consequence of high doses of amphetamines is a toxic neurosis with symptoms resembling paranoid schizophrenia. Abuse of amphetamines often occurs together with abuse of barbitur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/94G01N33/577G01N33/558G01N33/533
CPCG01N33/533G01N33/558G01N33/577G01N33/946
Inventor 贾嘉张振兴吕小翠赵琳周亮崔浩哲张鹏高翔
Owner 郑州左安检测科技有限公司
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