Microbial strain and screening method thereof and application in treating heavy metal polluted soil

A technology of polluted soil and screening method, applied in the field of microorganisms, to achieve the effect of good effect, small soil disturbance and strong tolerance

Active Publication Date: 2018-11-27
苏州中益世纪生态环境设计研究有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Microbial strain and screening method thereof and application in treating heavy metal polluted soil
  • Microbial strain and screening method thereof and application in treating heavy metal polluted soil
  • Microbial strain and screening method thereof and application in treating heavy metal polluted soil

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Effect test

Embodiment 1

[0033] The screening of microorganism bacterial strain of the present invention adopts liquid phase enrichment method, comprises the following steps:

[0034] (1) Weigh 5g of cadmium and copper polluted soil samples (Dongshan town agriculture and forestry planting field, Cd 2+ The content is 100mg / L, Cu 2+ content of 50 mg / L), added to the liquid medium containing beef extract peptone, added glass beads, and the Erlenmeyer flask was shaken at 28 ° C and 160 rpm to obtain a soil mixture;

[0035] (2) the soil mixture is transferred to a centrifuge tube, and the centrifuged supernatant is used as the source of cadmium and copper passivation microorganisms;

[0036] (3) the supernatant that step (2) obtains is inoculated into the beef extract peptone liquid medium (Cd) that contains cadmium and copper 2+ The content is 100mg / L, Cu 2+ content of 50mg / L), shake culture at 28°C and 160rpm, centrifuge after completion of cultivation, draw 2mL supernatant with a sterile pipette, tr...

Embodiment 2

[0045] Add different concentrations of Cd to beef extract peptone broth 2+ and Cu 2+ , sterilized at 121°C for 15min; then inoculate the microbial strains screened by the present invention (inoculum size is 2wt%); 2+ 、Cu 2+ When the concentration was 5mg / L, 20mg / L, 50mg / L, 100mg / L, the strain entered the logarithmic growth phase at 4, 5, 9, and 16 hours respectively, and the highest OD values ​​that could be achieved were 5.45, 4.99, 4.20 and 3.16, from the data show that the strain on Cd 2+ and Cu 2+ Still very well tolerated.

[0046] The above-mentioned beef extract-peptone liquid culture medium comprises the following components in mass percentage: 1% peptone, 0.5% beef extract, 0.5% sodium chloride, and the rest is distilled water.

Embodiment 3

[0048] The microbial strains screened by the present invention are inoculated in the beef extract peptone liquid medium, wherein the beef extract peptone liquid medium comprises the following components in mass percentages: 1% peptone, 0.5% beef extract, 0.5% sodium chloride, the rest It is distilled water, cultured to the logarithmic growth phase, and the bacterial liquid is obtained; or the bacterial liquid is freeze-dried with a vacuum freeze dryer for future use.

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Abstract

The invention discloses a microbial strain and a screening method thereof and an application in treating heavy metal polluted soil. The screened microbial strain is bacillus subtilis, the preservationnumber of which is CCTCC NO: M2017830. The application comprises the following steps: inoculating the microbial strain in a culture medium to be cultured to a logarithmic phase to obtain a bacterialsolution; adding the bacterial solution into the heavy metal polluted soil to be uniformly mixed to treat the heavy metal polluted soil; or adding the bacterial solution which is vacuum-frozen and dried into soil to be uniformly mixed to treat the heavy metal polluted soil; and fixing cadmium and copper in the polluted soil by means of passivation, wherein the bio-availability of cadmium is reduced and adsorption and utilization of cadmium by plants are reduced, so that the purpose of repairing soil is achieved.

Description

technical field [0001] The invention relates to the field of microbes, and relates to designing a microbial strain for repairing heavy metal polluted soil, a screening method and application thereof. Background technique [0002] According to the 2014 "National Soil Pollution Survey Bulletin", the overall situation of my country's soil environment is not optimistic. Man-made activities such as mining and agriculture are the main causes of soil pollution or exceeding standards. The total excess rate of soil in the country is 16.1%, and the pollution type is mainly inorganic. Among them, the cadmium pollutant point exceeding rate is the first among the 8 kinds of inorganic pollutants, accounting for 7.0%. The proportions of different levels of pollution points are: slight 5.2%, mild 0.8%, moderate 0.5%, and severe 0.5%. The distribution of cadmium pollutant content presents a trend of increasing gradually from northwest to southeast, and from northeast to southwest. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02B09C1/10C12R1/07
CPCB09C1/10C12N1/02C12N1/20C12N1/205C12R2001/075
Inventor 王佳斌蔡慧贺志刚程刚
Owner 苏州中益世纪生态环境设计研究有限公司
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