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Preparation method of uricase-modified titanium diboride composite paste electrode sensor

An electrode sensor, titanium diboride technology, which is applied in the field of electrochemical analysis, can solve the problems affecting the normal function of human cells, the acidification of human body fluids, and the retention of uric acid, and achieves the effects of low cost, small residual current and easy surface

Inactive Publication Date: 2018-11-27
山东政和产业大数据技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if there is too much uric acid in the body to excrete or the mechanism of uric acid excretion degrades, the body will retain too much uric acid. When the blood uric acid concentration is high, the body fluids will become acidic, affecting the normal function of human cells. If left alone for a long time, it will cause gout
In addition, excessive fatigue or lack of rest can also lead to relatively slow metabolism and lead to the onset of gout

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Nano TiB 2 / Preparation of carbon nanotube composite paste electrodes: separately added in agate mortar, nano-TiB 2 : 40g, carbon oxide nanotubes: 25g, 1-propenyl-3-methylimidazolium tetrafluoroborate: 28g, glycerol: 7g, grind evenly to obtain nano-TiB 2 / carbon nanotubes / 1-propenyl-3-methylimidazolium tetrafluoroborate mixture carbon paste; then put the carbon paste into a glass tube with an inner diameter of Φ3.5mm connected with wires, compact, dry, and use Polished with polishing powder and washed with deionized water to obtain nano-TiB 2 / Carbon nanotube composite paste electrode.

[0028] (2) 3-Chloropropyltrimethoxysilane-modified nano-TiB 2 / Carbon nanotube composite paste electrode preparation: In the reactor, add, respectively, absolute ethanol: 86 mL, 3-chloropropyltrimethoxysilane: 32 g, mix well, and mix the nano-TiB 2 / Carbon nanotube composite paste electrode was put in, the temperature was raised to 55±2 °C for constant temperature react...

Embodiment 2

[0032] (1) Nano TiB 2 / Preparation of carbon nanotube composite paste electrodes: separately added in agate mortar, nano-TiB 2 : 42g, carbon nanotubes: 23g, 1-propenyl-3-methylimidazolium tetrafluoroborate: 25g, glycerol: 10g, grind evenly to obtain nano-TiB 2 / carbon nanotubes / 1-propenyl-3-methylimidazolium tetrafluoroborate mixture carbon paste; then put the carbon paste into a glass tube with an inner diameter of Φ3.5mm connected with wires, compact, dry, and use Polished with polishing powder and washed with deionized water to obtain nano-TiB 2 / Carbon nanotube composite paste electrode.

[0033] (2) 3-Chloropropyltrimethoxysilane-modified nano-TiB 2 / Carbon nanotube composite paste electrode preparation: In the reactor, add, respectively, absolute ethanol: 82 mL, 3-chloropropyltrimethoxysilane: 35 g, mix well, mix the nano-TiB 2 / Carbon nanotube composite paste electrode was put in, the temperature was raised to 55±2 °C for constant temperature reaction for 6...

Embodiment 3

[0037] (1) Nano TiB 2 / Preparation of carbon nanotube composite paste electrodes: separately added in agate mortar, nano-TiB 2 : 38g, carbon oxide nanotubes: 26g, 1-propenyl-3-methylimidazolium tetrafluoroborate: 30g, glycerol: 6g, grind evenly to obtain nano-TiB 2 / carbon nanotubes / 1-propenyl-3-methylimidazolium tetrafluoroborate mixture carbon paste; then put the carbon paste into a glass tube with an inner diameter of Φ3.5mm connected with wires, compact, dry, and use Polished with polishing powder and washed with deionized water to obtain nano-TiB 2 / Carbon nanotube composite paste electrode.

[0038] (2) 3-Chloropropyltrimethoxysilane-modified nano-TiB 2 / Carbon nanotube composite paste electrode preparation: in the reactor, add, respectively, absolute ethanol: 89mL, 3-chloropropyltrimethoxysilane: 30g, mix well, the nano TiB 2 / Carbon nanotube composite paste electrode was put in, the temperature was raised to 55±2 °C for constant temperature reaction for 6 ...

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Abstract

The invention discloses a preparation method of a uricase-modified titanium diboride composite paste electrode sensor. The preparation method is characterized by comprising the following steps: firstly preparing a nano-TiB2 / carbon nanotube composite paste electrode from nano-TiB2, oxidized carbon nanotubes and 1-propenyl-3-methylimidazolium tetrafluoroborate; carrying out surface modification on the nano-TiB2 / carbon nanotube composite paste electrode by virtue of 3-chloropropyltrimethoxysilane, so as to obtain a 3-chloropropyltrimethoxysilane-modified nano-TiB2 / carbon nanotube composite pasteelectrode; and modifying the 3-chloropropyltrimethoxysilane-modified nano-TiB2 / carbon nanotube composite paste electrode by virtue of uricase stationary liquid prepared from a Tris buffer solution, gelatin and bovine serum albumin, so as to obtain the uricase-modified titanium diboride composite paste electrode sensor, wherein the pH value of the Tris buffer solution is 8.5. The electrode sensor prepared by virtue of the preparation method has higher conductivity than a common carbon paste electrode, has specific recognition ability to uric acid and is high in sensitivity.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method and application of a uricase modified titanium diboride composite paste electrode sensor. It belongs to the field of electrochemical analysis. Background technique [0002] Under normal circumstances, the uric acid in the body is about 1200 mg, about 600 mg is newly generated every day, and 600 mg is excreted at the same time, which is in a balanced state. However, if there is too much production in the body before it can be excreted or the uric acid excretion mechanism is degraded, the body will retain too much uric acid. When the blood uric acid concentration is high, the body fluids will become sour and affect the normal function of human cells. Long-term neglect will cause gout. In addition, excessive fatigue or insufficient rest can also lead to a relatively slow metabolism and lead to the onset of gout. Too high or too low uric acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N27/327G01N27/26G01N33/573
CPCG01N1/28G01N27/26G01N27/3271G01N33/573
Inventor 李慧芝李冬梅张卫民
Owner 山东政和产业大数据技术研究院
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