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Micro-fluidic chip and immunofluorescence analyzer

A microfluidic chip and immunofluorescence technology, applied in the direction of material analysis, material analysis, fluorescence/phosphorescence, etc. through optical means, can solve the problem of low sensitivity and achieve the effect of improving sensitivity

Pending Publication Date: 2018-11-27
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The inventors of the present application found in the long-term research process that traditional microfluidic chips use chemiluminescence to detect stacked samples, which has low sensitivity

Method used

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  • Micro-fluidic chip and immunofluorescence analyzer
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  • Micro-fluidic chip and immunofluorescence analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment one: detection of procalcitonin;

[0044]Procalcitonin (PCT) is a protein whose plasma levels are elevated in severe bacterial, fungal, and parasitic infections, as well as in sepsis and multiorgan failure. However, PCT will not increase in autoimmunity, allergy and virus infection. Local limited bacterial infection, minor infection and chronic inflammation do not cause it to be elevated. PCT reflects the active degree of systemic inflammatory response, and the test for PCT has significant clinical significance. For the detection of PCT, the double-antibody sandwich immunoluminescence method is widely used. The principle is to use double monoclonal antibodies, one of which is calcitonin antibody (primary antibody), which binds to the magnetic beads and the calcitonin part of the PCT molecule; The other is an anti-calcein antibody (secondary antibody), with the anti-calcein site labeled with fluorescein and PCT molecules. The two antibodies combine with PCT...

Embodiment 2

[0054] Embodiment 2, combined detection of BNP and troponin;

[0055] Clinical significance of combined detection of brain natriuretic peptide (BNP) and troponin Ⅰ (cTn Ⅰ) in auxiliary diagnosis of cardiac function. Usually, the detection of brain natriuretic peptide (BNP) and troponin I (cTnI) is performed twice, which increases the cost of detection on the one hand, and makes the detection more cumbersome and complicated on the other hand. For the detection of brain natriuretic peptide (BNP) and troponin I (cTnI), the double-antibody sandwich immunofluorescence method was used for detection in this example. , combined with brain natriuretic peptide (BNP) molecules, the surface of the primary antibody is modified with fluorescein; the second antibody is a secondary brain natriuretic peptide antibody, which binds to magnetic beads and another site of the brain natriuretic peptide (BNP) molecule. The detection principle of troponin Ⅰ (cTn Ⅰ) is the same as that of brain natriu...

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Abstract

The invention discloses a micro-fluidic chip and an immunofluorescence analyzer. The micro-fluidic chip comprises a substrate, wherein a micro-fluidic channel is formed in the substrate, and comprisesa trapping area; a plurality of concave parts are formed in the bottom of the trapping area and are distributed in a micro-array manner, are used for trapping micro-particle substances in a sample entering the trapping area, and have spaces capable of accommodating one or two micro-particle substance. By the mode, the micro-fluidic chip can be used for separating micro-particle substances in a sample, and the testing sensitivity can be improved.

Description

technical field [0001] The present application relates to the technical field of medical equipment, in particular to a microfluidic chip and an immunofluorescence analyzer. Background technique [0002] Microfluidic chip technology is a micron-scale structure processing micro (10 -9 L-10 -18 L) The reaction system of the sample is widely used in cell screening, immune detection, cell detection and analysis, etc.; the traditional microfluidic chip is characterized by the microstructure of the micropipeline network, and the micropipeline, micropump, microvalve Like integrated circuits, functional components such as micro-reservoirs and micro-detection elements are integrated on chip materials to complete sample processing and detection. [0003] The inventors of the present application have found in the long-term research process that traditional microfluidic chips use chemiluminescence to detect stacked samples, which has low sensitivity. Contents of the invention [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/64B01L3/00
CPCG01N21/64G01N21/6486G01N33/53B01L3/5027B01L2300/0861B01L2200/10
Inventor 蔡佳刘治志
Owner SHENZHEN DYMIND BIOTECH