Protein-coated fluorescent gold nanocluster as well as preparation method and application thereof
A technology of fluorescent gold nanometers and gold nanoclusters, which is applied in the field of preparation and protein-wrapped fluorescent gold nanoclusters, can solve problems such as complex reaction conditions, and achieve broad application prospects, good biocompatibility, detection function and therapeutic potential Effect
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Embodiment 1
[0034] Take 0.1 mL of 25 mM HAuCl 4 Add it into 2.36mL of 0.53mM aprotinin solution, and mix it thoroughly with a vortexer for 5min; then add 0.08mL of 1.0M NaOH solution into the tube, and mix it quickly with a vortexer to avoid precipitation. The mixing time is 5min. into a synthetic solution; the synthetic solution was airtight and protected from light, and shaken on a shaker at 90 rpm at 37° C. for 12 hours. After the reaction was completed, the sample was dialyzed in a 1000Da dialysis bag for 10 h, and then stored in the dark at 4°C for later use. Aprotinin and HAuCl 4 The molar ratio is 1:2.
[0035] The Ap-Au NCs that embodiment 1 makes, its morphology is as follows figure 1 As shown, it is a spherical shape with uniform size;
[0036] Such as figure 2 Shown: 118 Ap-Au NCs of gold nanocluster particles were measured, and the particle size distribution of Ap-Au NCs was found to be in the range of 2.25nm-3.25nm, and the average particle size was 2.84±0.6nm.
[0037]...
Embodiment 2
[0040] Take 0.1 mL of 25 mM HAuCl 4 Add it to 1.57mL of 0.53mM aprotinin solution, and mix it thoroughly with a vortex for 8 minutes; then add 0.06mL of 1.0M NaOH solution into the tube, and mix it quickly with a vortex to avoid precipitation, and mix for 8 minutes; The synthesized solution was shaken on a shaker at 90 rpm at 37° C. for 12 hours under airtight conditions and protected from light. After the reaction, the sample was dialyzed in a 1000Da dialysis bag for 36 hours, and then stored at 4°C in the dark until use. Aprotinin and HAuCl 4 The molar ratio is 1:3.
Embodiment 3
[0042] Take 0.1 mL of 25 mM HAuCl 4 Add it to 3.14mL of 0.53mM aprotinin solution, and mix it thoroughly with a vortex for 10 minutes; then add 0.1mL of 1.0M NaOH solution to the tube, and mix it quickly with a vortex to avoid precipitation, and mix for 10 minutes; The synthesized solution was shaken on a shaker at 90 rpm at 37° C. for 24 hours under airtight conditions and protected from light. After the reaction was completed, the sample was dialyzed in a 1000Da dialysis bag for 16 hours, and then stored in the dark at 4°C for later use. Aprotinin and HAuCl 4 The molar ratio is 1:1.5.
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