A kind of detection method of xylooligosaccharide in beverage

Abstract

The invention relates to quantitative detection of xylooligosaccharide in beverage, and specifically relates to a detection method of the xylooligosaccharide in the beverage. The method comprises thefollowing steps: (1) preparing a standard solution and performing liquid chromatography-tandem mass spectrometry to obtain a standard atlas of mixed standard solutions in different concentrations; (2)respectively manufacturing a concentration and chromatographic peak area relation curve chart through the standard atlas acquired in the step (1) according to the xylooligosaccharide variety; (3) pretreating the beverage sample and performing liquid chromatography-tandem mass spectrometry; (4) determining the content of various xylooligosaccharide in the sample, and determining the xylooligosaccharide variety through the retention time of a sample atlas obtained in the step (3) and comparing the chromatographic peak area of the xylooligosaccharide variety with the standard curve obtained in the step (2), and determining the xylooligosaccharide content by using an interpolation method. The detection method disclosed by the invention has the characteristics of being simple in pre-treatmentprocess, good in separation effect, fast in detection speed, accurate in detection result, high in sensitivity and good in detection reproducibility.

Description

technical field [0001] The invention relates to quantitative detection of xylooligosaccharides in beverages, in particular to a detection method of xylooligosaccharides in beverages. The invention belongs to the technical field of chemical analysis and instrumental analysis. Background technique [0002] Xylo-oligosaccharides are mainly made of xylan-rich plants such as bagasse, corn cobs, rice, cottonseed hulls, etc., and processed through a series of process steps. The main components are linked by β-1,4 glycosidic bonds It is an oligomeric mixed sugar composed of 2-7 xylose molecules. Xylo-oligosaccharide is a super bifidum factor, which is not absorbed by the human stomach, but it is beneficial to the growth of beneficial bacteria in the intestinal tract of humans and animals, and is not conducive to the growth of harmful bacteria. It is a food raw material with health care functions. [0003] Xylo-oligosaccharide has good physical and chemical properties: it is not ea...

AI Technical Summary

Problems solved by technology

[0004] Due to the complex composition of xylooligosaccharides, conventional chemical methods cannot be used to detect and analyze xylooligosaccharides. Thin layer chromatography, gas chromatography and liquid chromatography can be used to detect and analyze xylooligosaccharides. This method takes a long time, and it is more common to use liquid chromatography analysis. Usually, the sulfuric acid hydrolysis method (liquid chromatography) is used to indirectly calculate the xylose content. The calculation is more complicated, and it is impossible to measure the specific xylose to xylose sugar content

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