Method for rapid detection of aflatoxins in medicinal material and test paper card used in method

A technology of aflatoxin and medicinal materials, which is applied in the field of medical testing and medicine, and can solve the problems that affect the measurement results, complex components of Chinese medicinal materials, and cannot be directly applied.

Active Publication Date: 2018-11-30
BEIJING INST FOR DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] However, in the sample processing process of some traditional Chinese medicines before the determination of aflatoxin, due to the complex composition of Chinese medicinal materials, it may affect the results of the determination, so some impurities that interfere with the detection need to be removed during the sample processing process
[0021] Although the prior art di

Method used

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  • Method for rapid detection of aflatoxins in medicinal material and test paper card used in method

Examples

Experimental program
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Example Embodiment

[0049] Example 1: Method for detecting the total amount of aflatoxin in medicinal materials

[0050] (1) Provide colloidal gold test paper cards for the detection of aflatoxin (this example directly applies commercialized ROSA FAST5 aflatoxin quantitative rapid test strip, article number LF-AFQ-FAST5-100K, brand bioshome); the LF-AFQ-FAST5-100K includes: sample pad, nitrocellulose membrane (NC membrane), absorbent pad and PVC back Lining; the nitrocellulose membrane is adhered to the PVC backing, the sample pad and the absorbent pad are respectively adhered to the two ends of the nitrocellulose membrane; the nitrocellulose membrane is sequentially distributed with sample pads, test lines T1, test lines T2, and quality control Thread, absorbent pad;

[0051] (2) Sample extraction:

[0052] (2a) Take 2 grams of powder of the medicinal material to be tested or its decoction pieces, put it into a sample tube, and add 6 ml of 85% acetonitrile solution (which uses a phosphate ion concent...

Example Embodiment

[0063] Example 2: Method for detecting the total amount of aflatoxin in medicinal materials

[0064] (1) Provide colloidal gold test paper cards for detecting aflatoxin;

[0065] (2) Sample extraction:

[0066] (2a) Take 2 grams by weight of the powder of the medicinal material to be tested or its decoction pieces, put it into the sample tube, and add 4 ml of 90% acetonitrile solution (with a phosphate ion concentration of 12 mmol / L and a pH of 7.8). Phosphate buffer solution is added to the sample, vortex extraction for 3 minutes; after standing for 1 minute, centrifuge at 2000 rpm for 8 minutes, transfer 2 ml volume of the supernatant to the NH2 / C18 column (such as Grace-Alltech Adsorbosphere NH2 column) , Purify the sample supernatant through the column and collect it into a clean test tube;

[0067] (2b) Pipette 3 ml of methanol to the C18 column, install the syringe assembly, and discard after passing the column; pipette 3 ml of deionized water or distilled water to the C18 col...

Example Embodiment

[0072] Example 3: Method for detecting the total amount of aflatoxin in medicinal materials

[0073] (1) Provide colloidal gold test paper cards for detecting aflatoxin;

[0074] (2) Sample extraction:

[0075] (2a) Take 2 grams by weight of the powder of the medicinal material to be tested or its decoction pieces, put it into the sample tube, and add 8 ml by volume of 80% acetonitrile solution (which uses phosphoric acid with a phosphate ion concentration of 20 mmol / L and a pH of 7.3 Add salt buffer solution) to the sample, vortex for extraction for 1 minute; after standing for 1 minute, centrifuge at 5000 rpm for 3 minutes, transfer 2 ml volume of supernatant to NH2 / C18 column (such as Grace-Alltech Adsorbosphere NH2 column), Purify the sample supernatant through the column and collect it in a clean test tube;

[0076] (2b) Pipette 3 ml of methanol to the C18 column, install the syringe assembly, and discard after passing the column; pipette 3 ml of deionized water or distilled wa...

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Abstract

The invention relates to a method for rapid detection of aflatoxins in a medicinal material and a test paper card used in the method. The method includes the following steps: providing a colloidal gold test paper card for aflatoxin detection; extracting a sample with an acetonitrile solution, applying the sample to a NH2/C18 column for purifying, applying the sample to a C18 column for secondary,transferring the sample extract, applying the sample extract to the colloidal gold test paper card, performing incubation at constant temperature of 45DEG C, measuring test strip reading by a reader,and calculating the content of the aflatoxins in the medicinal material according to sample loading quantity and sample diluting time. The method can detect results in 5 minutes, and the method can accurately and quantitatively detect various root and rhizome traditional Chinese medicines. The test paper card has the characteristics of simple operation, high sensitivity, fast detection speed, accurate detection result and low cost, and is suitable for screening and on-site monitoring of a large number of samples. The method has high recovery rate, and the test results can be stored for a longtime.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to the technical field of medical detection, and relates to a method for rapidly detecting aflatoxin in Chinese medicinal materials, especially its total amount, and a test paper card used in the method. In particular, the present invention relates to a A method for rapidly detecting the amount of aflatoxin in Chinese medicinal materials, especially the total amount, by using a colloidal gold test paper card. The method of the invention exhibits excellent technical effects. Background technique [0002] Aflatoxin (AFT) is a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins. AFT is a secondary metabolite produced by toxin-producing strains such as Aspergillus flavus and Aspergillus parasiticus, and is the most stable class of toxins found in nature. There are more than 20 known AFTs, the common ones are aflatoxin B1, aflato...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 范妙璇傅欣彤杨文良郭洪祝陈有根
Owner BEIJING INST FOR DRUG CONTROL
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