Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapid detection of aflatoxins in medicinal material and test paper card used in method

A technology of aflatoxin and medicinal materials, which is applied in the field of medical testing and medicine, and can solve the problems that affect the measurement results, complex components of Chinese medicinal materials, and cannot be directly applied.

Active Publication Date: 2018-11-30
BEIJING INST FOR DRUG CONTROL
View PDF7 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] However, in the sample processing process of some traditional Chinese medicines before the determination of aflatoxin, due to the complex composition of Chinese medicinal materials, it may affect the results of the determination, so some impurities that interfere with the detection need to be removed during the sample processing process
[0021] Although the prior art discloses in detail the colloidal gold test strips that can be used for the detection of aflatoxins and even has finished products on the market, however, it cannot be directly applied to some Chinese medicinal materials for qualitative or even quantitative detection of aflatoxins. , especially there may be some impurities that affect the detection in Chinese herbal medicines

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapid detection of aflatoxins in medicinal material and test paper card used in method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: the method for detecting the total amount of aflatoxin in medicinal materials

[0050] (1) provide colloidal gold test paper card for detecting aflatoxin (this embodiment directly applies the commercialized ROSA FAST5 aflatoxin quantitative rapid detection strip, article number LF-AFQ-FAST5-100K, brand bioshome); the LF-AFQ-FAST5-100K includes: sample pad, nitrocellulose membrane (NC membrane), absorbent pad and PVC back lining; the nitrocellulose membrane is adhered to the PVC backing, and the sample pad and the absorbent pad are respectively adhered to the two ends of the nitrocellulose membrane; the sample pad, detection line T1, detection line T2, quality control line are distributed on the nitrocellulose membrane in sequence. line, absorbent pad;

[0051] (2) Sample extraction:

[0052] (2a) Get 2 grams by weight of the powder of the medical material to be tested or its decoction pieces, put it into the sample tube, add 6 milliliters of 85% aceton...

Embodiment 2

[0063] Embodiment 2: detect the method for aflatoxin total amount in medicinal material

[0064] (1) Provide colloidal gold test strips for the detection of aflatoxins;

[0065] (2) Sample extraction:

[0066] (2a) Get 2 grams by weight of the powder of the medical material to be tested or its decoction pieces, put it into the sample tube, add 4 milliliters of 90% acetonitrile solution by volume (it uses a solution with a phosphate ion concentration of 12mmol / L and a pH value of 7.8) Phosphate buffer solution) into the sample, vortex extraction for 3 minutes; after standing for 1 minute, centrifuge at 2000rpm for 8 minutes, pipette 2 ml volume supernatant to NH2 / C18 column (such as Adsorbosphere NH2 column of Grace-Alltech) , purify the sample supernatant through the column, and collect it into a clean test tube;

[0067] (2b) Pipette 3 ml of methanol to the C18 column, install a syringe assembly, pass through the column and discard; pipette 3 ml of volume deionized water ...

Embodiment 3

[0072] Embodiment 3: the method for detecting the total amount of aflatoxin in medicinal materials

[0073] (1) Provide colloidal gold test strips for the detection of aflatoxins;

[0074] (2) Sample extraction:

[0075] (2a) Get 2 grams by weight of the powder of the medical material to be tested or its decoction pieces, put it into the sample tube, add 8 milliliters of volume parts 80% acetonitrile solution (it uses phosphoric acid whose phosphate ion concentration is 20mmol / L and pH value is 7.3) Salt buffer solution) into the sample, vortex extraction for 1 minute; after standing for 1 minute, centrifuge at 5000rpm for 3 minutes, pipette 2 ml volume supernatant to NH2 / C18 column (such as Adsorbosphere NH2 column of Grace-Alltech), Purify the sample supernatant through the column and collect it into a clean test tube;

[0076] (2b) Pipette 3 ml of methanol to the C18 column, install a syringe assembly, pass through the column and discard; pipette 3 ml of volume deionize...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for rapid detection of aflatoxins in a medicinal material and a test paper card used in the method. The method includes the following steps: providing a colloidal gold test paper card for aflatoxin detection; extracting a sample with an acetonitrile solution, applying the sample to a NH2 / C18 column for purifying, applying the sample to a C18 column for secondary,transferring the sample extract, applying the sample extract to the colloidal gold test paper card, performing incubation at constant temperature of 45DEG C, measuring test strip reading by a reader,and calculating the content of the aflatoxins in the medicinal material according to sample loading quantity and sample diluting time. The method can detect results in 5 minutes, and the method can accurately and quantitatively detect various root and rhizome traditional Chinese medicines. The test paper card has the characteristics of simple operation, high sensitivity, fast detection speed, accurate detection result and low cost, and is suitable for screening and on-site monitoring of a large number of samples. The method has high recovery rate, and the test results can be stored for a longtime.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to the technical field of medical detection, and relates to a method for rapidly detecting aflatoxin in Chinese medicinal materials, especially its total amount, and a test paper card used in the method. In particular, the present invention relates to a A method for rapidly detecting the amount of aflatoxin in Chinese medicinal materials, especially the total amount, by using a colloidal gold test paper card. The method of the invention exhibits excellent technical effects. Background technique [0002] Aflatoxin (AFT) is a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins. AFT is a secondary metabolite produced by toxin-producing strains such as Aspergillus flavus and Aspergillus parasiticus, and is the most stable class of toxins found in nature. There are more than 20 known AFTs, the common ones are aflatoxin B1, aflato...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 范妙璇傅欣彤杨文良郭洪祝陈有根
Owner BEIJING INST FOR DRUG CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com