Application of ZINC62678696 in preparation of medicine for inhibiting liver fibrosis
A technology of liver fibrosis and drugs, applied in the field of preparation of drugs to inhibit liver fibrosis, can solve the problems of reversing liver fibrosis and no specific drugs
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Embodiment 1
[0017] ZINC62678696 Add pharmaceutically acceptable excipients and make various dosage forms according to conventional methods, such as liquid injections of various specifications, powder injections, emulsions for injections, tablets, pills, capsules, ointments, creams, patches, liniments , powder, spray, implant, drop, suppository, ointment, candy, etc.
[0018] The administration routes of ZINC62678696 include various administration routes: oral administration, injection administration, implant administration, intracavitary administration, sublingual administration, anal administration, transdermal administration, internal and external application, etc.
experiment example 1
[0019] Experimental example 1: gene chip screening and verification
[0020] Differential mRNAs in fibrotic liver tissue and normal liver tissue were screened by gene chip. The chip is currently the most powerful whole-transcriptome research tool, designed with 5.5 million probes, which basically include all known classic transcripts and all predicted possible gene transcription forms. In addition, the chip is designed with multiple probes for each sequence, which increases the reliability of the signal. We extracted total RNA from 5 fibrotic liver tissues and 5 normal liver tissues, and passed the quality inspection through high-throughput chip screening. According to the expression difference between fibrotic liver tissue and normal liver tissue, the expression difference was more than 1.6 times and the P value was less than or equal to According to the standard of 0.05, 1526 mRNAs were differentially expressed between fibrotic liver tissue and normal liver tissue, among wh...
experiment example 2
[0022] Experimental Example 2: Constructing CCl 4 and bile duct ligation (BDL)-induced liver fibrosis model in mice
[0023] CCl 4Induced mouse liver fibrosis model: purchase 36 balb / c male mice, and after a week of normal feeding, they were randomly divided into 6 groups, 6 in each group: control group (injected with olive oil) and CCl 4 2, 4, 6, 8, 10 week groups. CCl 4 Group mice were intraperitoneally injected with 0.3ml / kg CCl 4 (5%, v / v, dissolved in olive oil; ), twice a week for 8 weeks. After 8 weeks of treatment, the control group and CCl were anesthetized with chloral hydrate. 4 Group mice, recorded changes in liver appearance and photographed, and liver tissues were fixed and cryopreserved for subsequent experiments.
[0024] Bile duct ligation (BDL)-induced liver fibrosis model in mice:
[0025] (1) After buying 24 balb / c male mice and feeding them normally for one week, they were randomly divided into four groups with 6 mice in each group: control group (...
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