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dsRNA of wing development related gene optomotor-blind and application thereof in pest control

A technology of genes and DNA molecules, which is applied in the field of Bactrocera dorsalis control, can solve the problems that males cannot choose mates correctly, and achieve the effects of ecological security, good human health, prevention and control of invasion and spread

Inactive Publication Date: 2018-12-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RNA interference was first applied to Drosophila and developed. Relevant scholars used RNA interference technology to study the mating behavior of Drosophila and found that males lacking Genderblind protein in their bodies could not choose their mates correctly (Grosjean et al., 2007)

Method used

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  • dsRNA of wing development related gene optomotor-blind and application thereof in pest control
  • dsRNA of wing development related gene optomotor-blind and application thereof in pest control
  • dsRNA of wing development related gene optomotor-blind and application thereof in pest control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. Obtaining dsRNA of wing development-related gene Optomotor blind (abbreviated omb)

[0051] 1. Samples of Bactrocera dorsalis for testing

[0052] The insect source Bactrocera dorsalis used in this experiment is the indoor population of the Plant Quarantine and Invasion Biology Laboratory of China Agricultural University. The indoor breeding conditions: the temperature is 25°C; the relative humidity is kept at about 70%; D). The breeding method of Bactrocera dorsalis is as follows: Bactrocera dorsalis is reared uniformly in the insect cage, and the larval feed (the formula of the larval feed is shown in Table 1) is added to 500mL distilled water, boiled and bottled for later use, and it is frozen after being cooled. ; Adult feed (adult feed is obtained by mixing sucrose and soybean peptone in a mass ratio of 1:3) is fed to adults after adding water and moistening. After feeding on the feed, the larvae burrow into the soil to pupate after they are mature, an...

Embodiment 2

[0157] Embodiment 2, the application of the dsRNA of wing development-related gene optomotor blind in the control of Bactrocera dorsalis

[0158] 1. Feeding dsRNA

[0159] The 1st instar healthy and normally developing Bactrocera dorsalis larvae were selected as experimental materials. The experiment was set up with blank control group, dsGFP (irrelevant gene) control group and treatment group. Each group had three replicates, with 40 larvae in each replicate.

[0160] 1. Blank control group (CK)

[0161] Take 20 1.5mL centrifuge tubes, add about 0.15g crushed B. dorsalis larva feed and 4 μL ddHO to each centrifuge tube 2 O, mix well. Two larvae were placed in each centrifuge tube for feeding. The feeding and growth of larvae were observed daily. After 48 hours, the feed is completely eaten, and then only supplementary feed is sufficient until the larvae grow to the end of the third instar. One of the surviving larvae in the centrifuge tube was picked into the soil to pupat...

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Abstract

The invention discloses a dsRNA of a wing development related gene optomotor-blind and an application thereof in pest control. Primers are designed for the optomotor blind of wing development of citrus fruit fly and dsRNA is synthesized for the first time, and RNAi is carried out on the optomotor blind gene of citrus fruit fly by applying a feeding method, so that deformed phenotype of the wing development is obtained on the premise of ensuring interference efficiency. The dsRNA not only solves the problem that injection and introduction of dsRNA are hard to operate in the field, thereby laying a foundation for controlling and preventing citrus fruit fly by directly spraying an RNA preparation for future research, and also achieving good economical and social benefits in effective prevention of invasion and diffusion of the citrus fruit flies , protection of agricultural production and ecological safety of china and human health.

Description

technical field [0001] The invention relates to the fields of biotechnology and agricultural pest control, in particular to the dsRNA of the wing development-related gene optomotorblind (omb) and its application in pest control, in particular to its application in the control of Bactrocera dorsalis. Background technique [0002] Bactrocera dorsalis Hendel belongs to Diptera, Tephritidae, and Bactrocera, and is an important pest of many tropical and subtropical fruits and vegetables. The expansion situation is grim. Bactrocera dorsalis is an omnivorous pest with a wide range of hosts. It can harm more than 250 kinds of fruits and vegetables in more than 40 families such as citrus, guava, carambola, mango, banana, eggplant, pepper, and melon (Deng Zhisheng, 2001; Shang Hong Sheng, 1997), in 2014, the Ministry of Agriculture of my country listed it as a key agricultural invasion target, and the control strategy of Bactrocera dorsalis has received widespread attention. At prese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A01N57/16A01P7/04
CPCA01N57/16C12N15/113C12N2310/14
Inventor 李志红郭韶堃沈杰
Owner CHINA AGRI UNIV
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