Visual detection method for ultrafast amplification of copper and calcium-cleaved functional nucleic acids with universal isolation

An amplification system and fast technology, applied in the field of biosensors, can solve the problems of expensive, cumbersome sample processing, and lack of universal applicability
CN108949932BActive Publication Date: 2020-07-28CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-07-28

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Abstract

The invention provides a universal-partition ultrafast-amplification cleavage type functional-nucleic acid visual detection method for copper and calcium. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can be realized in the presence of copper and calcium ions, and amplification products are allowed to form G-quadruplexes in a proper environment; and color development is performed by utilizing the peroxidase-like activity of the G-quadruplexes. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of copper and calcium ions is realized. Moreover, the method provided in the invention has the characteristics of high specificity and high sensitivity to copper and cadmium ions, and is more objective and accurate in detection results.
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Description

technical field

[0001] The invention relates to the technical field of biosensors, in particular to a method for visual detection of copper and calcium-cutting functional nucleic acids with universal partition ultrafast amplification. Background technique

[0002] Copper is a transition element, chemical symbol Cu, atomic number 29, atomic weight 63.546, belonging to group IB. Pure copper is a soft metal, its surface is reddish orange with metallic luster when it is just cut, and its single substance is purplish red. Good ductility, high thermal conductivity and electrical conductivity. The amount of copper in the normal human body is 100-200 mg. In the human body, copper ions are mainly used as catalytic cofactors or structures for many enzymes and proteins, and are widely involved in many important metabolisms in the body. The process affects the generation of human blood, the formation of connective tissue, the central nervous system, the metabolism of cholesterol and gl...

Claims

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