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Method for detecting lincomycin in cow milk

A lincomycin and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex processing process and inaccurate characterization, and achieve the effect of simplifying pre-processing steps, reducing detection costs, and complete extraction

Inactive Publication Date: 2018-12-11
蒙牛乳业泰安有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pretreatment process of high performance liquid chromatography and gas chromatography is complicated and cannot be accurately qualitative
Although enzyme-linked immunosorbent assay (ELISA) and colloidal gold immunochromatography test strip method are simple and fast to operate, they are only suitable for the screening of a large number of samples and cannot be used for accurate qualitative and quantitative detection.

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  • Method for detecting lincomycin in cow milk
  • Method for detecting lincomycin in cow milk
  • Method for detecting lincomycin in cow milk

Examples

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Embodiment Construction

[0072] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0073] A method for detecting lincomycin in milk, comprising the following steps:

[0074] a. Weigh 4g sample, put it in a 50ml centrifuge tube, add 20ml acetonitrile, vortex mix for 30s, ultrasonically extract in an ice-water bath for 10min, centrifuge at 8000r / min at 4°C for 5min, transfer the supernatant to another 50ml centrifuge tube ;

[0075] b. Repeat the extraction with 10ml of acetonitrile again, and combine the supernatant into the same centrifuge tube;

[0076] c. Add 10ml of n-hexane to the supernatant, vortex and mix for 1min, centrifuge at 8000r / min at 4°C for 5min, discard the n-hexane layer, and transfer all the lower layer solution to a chicken heart bottle;

[0077] d. Rotary evaporate at 45°C to near dryness, wash the heart bottle with acetonitrile aqueous solution (acetonitrile and ...

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Abstract

The invention relates to the field of food safety detection, and particularly relates to a method for detecting lincomycin in cow milk. According to the method, lincomycin remained in milk can be quickly and accurately subjected to qualitative and quantitative analysis by a liquid chromatography-tandem mass spectrometry method; the sample is subjected to secondary extraction with acetonitrile, n-hexane is added into an extracting liquid to remove fat therein, and lincomycin in cow milk can be detected by the high-efficient liquid chromatography-tandem mass spectrometry method.

Description

technical field [0001] The invention relates to the field of food safety detection, in particular to a detection method for lincomycin in milk. Background technique [0002] Lincomycin (lincomycin, LIN), also known as lincomycin and lincomycin, is a lincosamide antibiotic produced by the fermentation of Streptomyces lincoln. It is mainly effective against Gram-positive bacteria, certain anaerobic bacteria and mycoplasma Strong antibacterial effect, but ineffective against Gram-negative bacteria. It is often used as a feed additive or directly injected into the udder of cows to treat mastitis. Both methods of administration will cause lincomycin to remain in the cow’s milk. The milk is excreted, resulting in a higher concentration of drug residues in the milk. Residual lincomycin in milk can cause renal dysfunction and increase drug resistance of Gram-positive bacteria after entering the human body through the food chain. Therefore, Announcement No. 235 of the Ministry of ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047G01N2030/062
Inventor 孙健郭晓梅王连凤孟庆红陈志斌
Owner 蒙牛乳业泰安有限责任公司