Compound water-retaining agent for Chinese-style meat dishes, preparation method thereof and application thereof
The technology of a water-retaining agent and meat is applied in the field of compound water-retaining agent for Chinese-style meat dishes and its preparation to achieve the effects of improving whiteness, improving structural quality and improving water-holding capacity.
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Embodiment 1
[0028] 1. Carrageenan oligosaccharide nano-liposome: mix soybean lecithin and cholesterol according to the mass ratio of 1:3, and dissolve it in the external aqueous phase ether solvent, and take a carrageenan oligosaccharide solution with a concentration of 10 mg / mL As the inner water phase, mix according to the volume ratio of the outer and inner water phases of 4:1.25, homogenize once in a dynamic high-pressure micro-fluidizer at 80MPa and 45°C for 5 minutes to form a stable emulsion, and transfer the emulsion into an evaporating bottle 35°C rotary evaporation until ether evaporates to dryness, then add 300~500mL PBS buffer solution and Tween 80 with 1% solution volume, continue rotary evaporation to evaporate all organic solvents, and then ultrasonic treatment for 20 min to obtain carrageenan oligoglycolipid plastid.
[0029] 2. Secondary homogenization: add 0.2% carrageenan oligosaccharide nanoliposome, 0.2% soybean protein isolate, 0.1% trisodium citrate, 0.4% sodium cas...
Embodiment 2
[0033] 1. Carrageenan oligosaccharide nano-liposome: mix soybean lecithin and cholesterol according to the mass ratio of 1:3, and dissolve it in the external aqueous phase ether solvent, and take a carrageenan oligosaccharide solution with a concentration of 10 mg / mL As the inner water phase, mix according to the volume ratio of the outer and inner water phases of 4:1.25, homogenize once in a dynamic high-pressure micro-fluidizer at 80MPa and 45°C for 5 minutes to form a stable emulsion, and transfer the emulsion into an evaporating bottle 35°C rotary evaporation until ether evaporates to dryness, then add 300~500mL PBS buffer solution and Tween 80 with 1% solution volume, continue rotary evaporation to evaporate all organic solvents, and then ultrasonic treatment for 20 min to obtain carrageenan oligoglycolipid plastid.
[0034] 2. Secondary homogenization: Add 0.4% carrageenan oligosaccharide nanoliposomes, 0.3% soybean protein isolate, 0.2% trisodium citrate, 0.6% sodium ca...
Embodiment 3
[0038] 1. Carrageenan oligosaccharide nano-liposome: mix soybean lecithin and cholesterol according to the mass ratio of 1:3, and dissolve it in the external aqueous phase ether solvent, and take a carrageenan oligosaccharide solution with a concentration of 10 mg / mL As the inner water phase, mix according to the volume ratio of the outer and inner water phases of 4:1.25, homogenize once in a dynamic high-pressure micro-fluidizer at 80MPa and 45°C for 5 minutes to form a stable emulsion, and transfer the emulsion into an evaporating bottle 35°C rotary evaporation until ether evaporates to dryness, then add 300~500mL PBS buffer solution and Tween 80 with 1% solution volume, continue rotary evaporation to evaporate all organic solvents, and then ultrasonic treatment for 20 min to obtain carrageenan oligoglycolipid plastid.
[0039] 2. Secondary homogenization: add 0.5% carrageenan oligosaccharide nanoliposomes, 0.3% soybean protein isolate, 0.2% trisodium citrate, 0.5% sodium ca...
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