Penaeus monodonantibacterial peptideALFpm10 and preparation method thereof

Abstract

The invention belongs to the technical field of crustacean genetic engineering, and discloses a Ppenaeus monodonantibacterial peptide ALFpm10 and a preparation method thereof. The Ppenaeus monodonantibacterial peptide ALFpm10 is prepared from 132 amino acids, the molecular weight is 15.3 kDa, and the signal peptide is prepared from 27 amino acids; the full-length cDNA of the ALFpm10 antibacterialpeptide is prepared from 517 bases, and obtained from RT-PCR cloning in the total RNA of a Penaeus monodon blood cell; the ALFpm10 antibacterial peptide can be obtained by fusion expression with SUMOprotein through a prokaryotic expression system, which is a solid phase chemosynthetic method. The ALFpm10 antibacterial peptide disclosed by the invention has antibacterial and bactericidal effects against Staphylococcus aureus, coastal Exiguobacterium,algae-occupying Bacillusand the like, and has the characteristics of high bactericidal efficiency, small usage amount, long lasting, and capable of suppressing seawater bacteria, and shows a broad application prospect in the aquaculture industry.

Description

technical field [0001] The invention belongs to the technical field of crustacean genetic engineering, and in particular relates to an antibacterial peptide ALFpm10 of Penaeus monodon and a preparation method thereof. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] In recent years, the aquaculture and fishing output in my country's fishery has jumped to the forefront of the world, and the fishery economy has developed very rapidly, and aquaculture accounts for 70% of the global aquaculture. However, with the development of intensive and semi-intensive aquaculture models in China and the increasing degree of intensification, eutrophic factors such as residual bait, feces, and aquatic animal and plant carcasses coexist in one water body, coupled with water pollution and The reduction of self-purification and regulation ability of ponds has led to severe excess of chemical oxygen demand, biological oxyg...

AI Technical Summary

Problems solved by technology

[0010] Therefore, the antimicrobial peptides derived from prawns have the following advantages: ①Good biological safety, the antimicrobial peptides derived from prawns themselves will not cause harm to prawn farming and the water environment; ; ③ It can inhibit and kill bacteria from the ocean. Many marine bacteria can tolerate stress such as high salt, resulting in poor antimicrobial peptides from other sources.

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