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A strain of urea-producing bacteria and its application

A technology of urea and bacterial strains, applied in urea-producing bacteria and its application fields, to achieve the effect of promoting growth, efficient crop growth, and low cost of use

Active Publication Date: 2021-05-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the recent research, the present inventor found for the first time that the 1433 strain can produce urea on the conventional culture medium, and has a significant growth-promoting effect on tomato, flue-cured tobacco and other crops, and is an important strain for the development of biological fertilizers. And its application on microbial fertilizer has not been reported

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  • A strain of urea-producing bacteria and its application
  • A strain of urea-producing bacteria and its application
  • A strain of urea-producing bacteria and its application

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Embodiment Construction

[0020] Below in conjunction with embodiment the present invention is described in further detail. The microbial fertilizer in the embodiment is made by the conventional method of microbial fermentation and the conventional method of microbial fertilizer preparation.

[0021] 1. Determination of urea production by strain 1433

[0022] Draw the standard curve of urea content: Prepare the urea aqueous solution with mass concentration of 20mg / L, 50mg / L, 100mg / L, 200mg / L, 300mg / L respectively. Add 5.4ml mixed reagent in brown colorimetric tube (mixed reagent formula: 120mg thiosemicarbazide, 125mg Ce(SO 4 ) 2 2(NH 4 ) 2 SO 4 , 55ml of concentrated sulfuric acid, 46ml of 85% phosphoric acid, 380mg of 4-antipyrine, fixed to 1000ml with deionized water), respectively add 100 μL of urea aqueous solution of each concentration in the colorimetric tube, then add 540 μL of mass concentration to be 1.2% Diacetyl monoxime aqueous solution, after mixing, 100 ℃ water bath for 8 minutes, ...

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Abstract

The invention relates to a urea-producing bacterium and its application, and belongs to the technical field of microbial fertilizers. The urea-producing bacterium used is the 1433 strain of Bacillus zanthoxyli, which has been preserved in the China Center for Type Culture Collection on June 14, 2018; the address of the preservation unit: China. Wuhan. Wuhan University; preservation number: CCTCC No: M 2018369. The capsicum bacillus (B. zanthoxyli) 1433 bacterial strain of the present invention can produce urea under conventional microbial culture conditions, and its bacterial body and metabolites are used in the preparation of microbial fertilizers. The beneficial effect of the present invention is that when the microbial fertilizer prepared by the 1433 strain is used to replace urea, the yield increase rate of flue-cured tobacco and tomato is still more than 20%, and it has the characteristics of low use cost, good yield increase effect, no residue, and safety for humans, animals and the environment .

Description

technical field [0001] The invention relates to a urea-producing bacterium and its application, belonging to the technical field of microbial fertilizers. Background technique [0002] Nitrogen is an important element necessary for the normal growth and development of plants. Nitrogen fertilizer contributes 40% to the increase in grain production, and is the dominant factor limiting crop yield in agricultural production (Jones et al., 2005). The supply of nitrogen in the vast majority of cultivated land in my country is insufficient, and it is necessary to continuously increase the application of nitrogen fertilizer to ensure the sustained high yield of crops (Liu Chunhong et al., 2003). Urea is currently the most widely used nitrogen fertilizer variety with the largest amount of application in agricultural production in the world, accounting for about 50% of the world's nitrogen fertilizer production and consumption market. However, long-term excessive application of nitro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C05G1/00C12R1/07
CPCC05B1/02C05F11/08C05G3/00C12N1/205C12R2001/07C05D1/02C05C9/00C05D9/00
Inventor 莫明和祝明亮茶琦雁刘子仪张体坤王学坚马莉夏振远
Owner YUNNAN UNIV