Mulberry fungus strain cultivation method

A cultivation method and mulberry technology, applied in the field of mulberry strain cultivation, can solve problems such as insufficient product quality, low acceptance by growers, difficulty in promotion, etc., so as to reduce cultivation costs, reduce excessive dependence, and improve product quality. quality effect

Inactive Publication Date: 2018-12-21
四川尚好桑业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems such as low yield and insufficient product quality in the cultivation of edible fungi using mulberry branches. Therefore, it is difficult to promote and the acceptance of growers is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The cultivation side of the present embodiment Morangibacterium bacterial classification comprises the following steps:

[0019] (1) A method for cultivating Morangibacterium strains, including the separation and cultivation of the mother species: wipe the surface of the mushroom buds that meet the requirements for growing mushrooms with 75% alcohol, then use a knife to cut the mushroom buds longitudinally into two parts, and Cut off 0.5cm at the junction of the cap and the sperm 3 A small piece of bacterial meat was placed in the center of the slant culture medium. After inoculation, the test tube was placed in an incubator, and cultured at 23°C for 7 days in a dark environment, and the relative humidity of the air was kept at 70%. After the silk grows on the slant, it is moved to a new slant medium and cultivated into a mother species;

[0020] (2) Cultivation of original species and cultivated species: pick a large piece of broad bean from the cultivated mother spec...

Embodiment 2

[0025] The cultivation side of the present embodiment Morangibacterium bacterial classification comprises the following steps:

[0026] (1) A method for cultivating Morangibacterium strains, including the separation and cultivation of the mother species: wipe the surface of the mushroom buds that meet the requirements for growing mushrooms with 75% alcohol, then use a knife to cut the mushroom buds longitudinally into two parts, and Cut off 0.5cm at the junction of the cap and the sperm 3 A small piece of bacterial meat was placed in the center of the slant medium. After inoculation, the test tube was placed in an incubator, and cultured at 24°C for 10 days in a dark environment, and the relative air humidity was kept at 65%. After the silk grows on the slant, it is moved to a new slant medium and cultivated into a mother species;

[0027] (2) Cultivation of original species and cultivated species: pick a large piece of broad bean from the cultivated mother species with an in...

Embodiment 3

[0032] The cultivation side of the present embodiment Morangibacterium bacterial classification comprises the following steps:

[0033] (1) A method for cultivating Morangibacterium strains, including the separation and cultivation of the mother species: wipe the surface of the mushroom buds that meet the requirements for growing mushrooms with 75% alcohol, then use a knife to cut the mushroom buds longitudinally into two parts, and Cut off 0.5cm at the junction of the cap and the sperm 3 A small piece of bacterial flesh was placed in the center of the slant culture medium. After inoculation, the test tube was placed in an incubator, and cultured at 22°C for 8 days in a dark environment, and the relative air humidity was kept at 80%. After the silk grows on the slant, it is moved to a new slant medium and cultivated into a mother species;

[0034] (2) Cultivation of original species and cultivated species: pick a large piece of broad bean from the cultivated mother species wi...

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PUM

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Abstract

The invention discloses a mulberry fungus strain cultivation method. The method comprises the following steps that contextus is cut from mushroom buds meeting various mushroom requirements for cultivation to obtain a mother strain, the cultivated mother strain is inoculated into an oblique plane culture medium, cultivation is conducted under proper conditions, a stock is obtained, the stock is subjected to amplification culture, after cultivation, the strain is pressed to be bacterial spawn, then a culture material bacteria stick is made from mulberry sawdust, miscellaneous sawdust, wheat bran, silkworm manure, monopotassium phosphate, gypsum and saccharose, after sterilization, the bacterial spawn is inoculated onto the culture material bacteria stick and put into a greenhouse with properconditions, controlling is conducted to achieve the proper conditions, and an ordinary method is adopted for the subsequent planting process. The method has the advantages that the mulberry sawdust made from mulberry branches is adopted for replacing partial miscellaneous sawdust to make the culture material, over-dependence on forest resources can be reduced, the fungus-forest contradiction is relieved, mulberry branches can be effectively utilized, and the edible fungus cultivation cost is lowered; meanwhile, the mulberry sawdust contains rich mineral elements, and the product quality of the edible fungus can be improved.

Description

technical field [0001] The invention relates to the field of strain cultivation, in particular to a method for cultivating Morangi fungus strains. Background technique [0002] In recent years, my country's edible fungus industry has developed rapidly, but at present there is a shortage of wood, and the contradiction between mushroom forests is becoming more and more serious in the process of cultivating and planting edible fungi. Although some cottonseed shells are used to replace miscellaneous wood chips, this problem cannot be fundamentally solved. The sericulture industry in my country is relatively large-scale, and mulberry trees are widely planted in some parts of southern China. The number of branches discarded in the summer pruning of mulberry trees every year is astonishing. These branches are often piled up on the ground and burned or left to rot in the wasteland. Mulberry branches are rich in 16 kinds of mineral elements such as potassium, calcium and magnesium, es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20A01G18/00
CPCA01G18/00A01G18/20A01G18/40
Inventor 刘露民刘惠民程博赵庆华
Owner 四川尚好桑业有限公司
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