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Apolipoprotein E test kit

A detection kit and apolipoprotein technology, applied in the field of apolipoprotein E detection kits, can solve the problem of limiting the application of apolipoprotein E, affecting the stability and accuracy of detection of apolipoprotein E, and the interference of inaccurate detection, etc. problem, to achieve the effect of improving the detection stability and detection accuracy, and facilitating the application of the detection effect

Inactive Publication Date: 2018-12-28
浙江强盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the conventional detection of apolipoprotein E, it is very easy to cause inaccurate interference due to concentration or other individual differences, which affects the stability and accuracy of detection of apolipoprotein E, and greatly limits the detection of apolipoprotein E. Application of Protein E

Method used

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Embodiment Construction

[0011] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the examples in the implementation manners, other examples obtained by those skilled in the art without making creative efforts all belong to the protection scope of this patent.

[0012] A detection kit for apolipoprotein E comprises reagent R1 and reagent R2, said reagent R1 comprises glycine buffer solution 50-80mmol / L, polyoxyethylene 20-30mmol / L, sodium edetate 15-28mmol / L , dodecylphenol 27-35mmol / L, purine nucleoside phosphorylase 3-8u / L, xanthine oxidase 6-10u / L, sodium benzoate 20-35mmol / L, glyceraldehyde 4.5-6.8g / L , Potassium dihydrogen phosphate 10-15mmol / L;

[0013] The R2 reagent includes glycine buffer 30-50mmol / L, latex particle...

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Abstract

The invention discloses an apolipoprotein E test kit, comprising a reagent R1 and a reagent R2. The reagent R1 comprises a glycine buffer solution, polyoxyethylene, sodium ethylene diamine tetracetate, purine nucleoside phosphorylase, dodecyl phenol, xanthine oxidase, sodium benzoate, glyceraldehyde, potassium dihydrogen phosphate. The reagent R2 comprises a glycine buffer, a latex particle of apolipoprotein E monoclonal antibody, nonidet p40, potassium chloride, calcium acetate, adenosine, disodium hydrogen phosphate, sodium ethylene diamine tetracetate, hypoxanthine nucleotide. The apolipoprotein E test kit can accurately measure in vitro, and is effectively applied to clinical auxiliary diagnosis and screenings for diseases such as hyperlipemia, coronary heart disease, hepatopathy, acute hepatitis, biliary cirrhosis, etc., and has excellent detecting stability and detecting accuracy.

Description

technical field [0001] The invention relates to a detection kit for apolipoprotein E. Background technique [0002] Apolipoprotein E (apolipoprotein E, ApoE) is a polymorphic protein that participates in the transformation and metabolism of lipoproteins. Its genes can regulate many biological functions and are related to the pathogenesis of many ophthalmic diseases. ApoE and its gene polymorphisms Sexual research is one of the hotspots in medical research at present, exploring the inner relationship between the two has important clinical application value for the prevention, diagnosis and treatment of eye diseases. Apolipoprotein E mainly exists in CM, VLDL, IDL and part of HDL, and the normal human plasma ApoE concentration is 0.03-0.05g / L. ApoE concentrations are positively correlated with plasma triglyceride levels. At present, in the conventional detection of apolipoprotein E, it is very easy to cause inaccurate interference due to concentration or other individual dif...

Claims

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Application Information

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IPC IPC(8): G01N33/92G01N33/577
CPCG01N33/92G01N33/577
Inventor 曹春梅钱静黄信用
Owner 浙江强盛生物科技有限公司
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