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A kind of breeding method of male parent of mating line of high multiplication type tilapia

A technology of tilapia and matching lines, applied in fish farming, application, climate change adaptation, etc., to achieve the effects of improving fecundity, avoiding blindness, and ensuring male rate

Active Publication Date: 2020-12-29
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the parents obtained by this method are still pure lines of Olia tilapia and Nile tilapia in germplasm, which cannot change biologically. Due to the nature of interbreeding, with the increase of selective breeding generations, there is limited room for further improvement of reproductive performance of mating lines

Method used

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  • A kind of breeding method of male parent of mating line of high multiplication type tilapia
  • A kind of breeding method of male parent of mating line of high multiplication type tilapia
  • A kind of breeding method of male parent of mating line of high multiplication type tilapia

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Experimental program
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Effect test

Embodiment

[0050] like figure 1 Shown, the breeding method of the male parent of the high-proliferation type tilapia mating line of the present embodiment comprises the steps:

[0051] Step 1: Establishment of the F1 hybrid population

[0052] like figure 2 As shown, the principle of this step is explained: the male tilapia ZX♂ individual and the male tilapia ZX♂ individual are crossed to produce four sex chromosome types of hybrid F1 generation XZ♂, ZZ♂, XW♀ and ZW♀.

[0053] Select 20 sexually mature male tilapia ZX♂ individuals over 10 months old, with a total length of 25.0cm and above, and a weight of 400g and above. , 40 sexually mature female Olia tilapia ZW♀ individuals, at 200m 2 Carry out hybridization in the cement pond (mud bottom), obtain hybrid F1 generation population, select the seedling with strong vigor to total 2000 tails, cultivate to more than 200 days old. The hybrid F1 population includes male ZX♂ individuals, male ZZ♂ individuals, female XW♀ individuals and ...

experiment example

[0070] Step 1: Breeding test of high-propagation tilapia mating lines

[0071] Use 200m 2 The cement pool (mud bottom) was used as the breeding test pool, and a control group and an experimental group were set up, with 3 repetitions in each group. Wherein, the female parent of the control group adopts female Nile tilapia, and the male parent adopts male Olia tilapia for hybridization; The male parent of high-proliferation tilapia.

[0072] The fry were collected on the 15th day after pairing, 2 times a day, continuously collected for 15 days, and the average seedling yield of each pond was calculated. The results showed that the average seedling yield of the test group was 5553.0 fish per day, and the average seedling yield of the control group was 3001.3 tails. Tail / day, the test group is 85.02% higher than the control group, and the benefit is remarkable. The specific data are shown in Table 2.

[0073] Table 2 Breeding test of high-propagation type tilapia mating line s...

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Abstract

The present invention discloses a breeding method for male parent of a high-prolificacy tilapia synthetic line, and belongs to the technical field of breeding of tilapia. The breeding method for the male parent of the high-prolificacy tilapia synthetic line comprises the following steps: step 1 : development of F1 hybrid population; step 2, screening of ZZ individuals in the F1 hybrid population;step 3, screening of heterozygous ZW individuals; and step 4, breeding of the male parent of the high-prolificacy tilapia synthetic line. According to the present invention, hybrid tilapia having sexchromosomes of the type ZZ and autosomes comprising those of nile tilapia are bred through the steps of hybridization, crossbreeding, backcrossing and the like, and the genetic relationship between the female parent and the male parent of the synthetic line is allowed to get closer; thus, the prolificacy of the synthetic line is improved, and the male rate of the first filial generation is also ensured.

Description

technical field [0001] The invention relates to a method for selecting male parents of a high-propagation type tilapia mating line, and belongs to the technical field of tilapia breeding. Background technique [0002] Tilapia is one of the important freshwater aquaculture species in my country, and the genetic improvement of its economic traits plays an important role in industrial development. In terms of sex chromosomes, there are two types of sex-determining chromosomes in tilapia, one is male heterogeneity, namely XX♀ and XY♂, and the other is female heterogeneity, namely ZW♀ and ZZ♂ type. Studies have shown that the order of sex-determining ability of sex chromosomes is Y>W>Z>X. The Oria tilapia male fish (ZZ) and the Nile tilapia female fish (XX) are used to form a matching line to obtain the first generation (ZX) through interspecific hybridization by taking advantage of this characteristic. It has strong disease resistance and cold resistance, the natural...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 杜雪松黄姻文露婷陈忠覃俊奇林勇彭金霞王卉
Owner GUANGXI ACADEMY OF FISHERY SCI