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Plant extract and preparation method and application thereof

A technology of plant extracts and substances, applied in the field of plant extracts and their preparation, can solve problems such as scarcity of resources and poor efficacy of medicinal materials, and achieve the effects of simple and convenient operation steps, resisting organ aging, and delaying skin aging

Pending Publication Date: 2019-01-04
SHANGHAI SHENG WEI BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a plant extract and its preparation method and application in view of the shortage of traditional Chinese medicinal materials that can significantly inhibit skin aging in the prior art, while the medicinal materials with abundant resources are not effective.

Method used

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  • Plant extract and preparation method and application thereof
  • Plant extract and preparation method and application thereof
  • Plant extract and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The Tremella fungus involved in the present invention is a widespread species, known in modern technology, and can be obtained from commercial channels. This patent randomly purchased two strains in the market, strain 1: white fungus preserved in the China Forestry Microorganism Culture Collection Management Center, No.: CFCC 87563; strain 2: white fungus preserved in the China Agricultural Microbiology Culture Collection Center , No.: ACCC 50546.

[0041] 1. Liquid fermentation culture

[0042] Liquid medium: potato extract 1.0L, glucose 20.0g, KH 2 PO 4 3.0g, MgSO 4 .7H 2 O 1.5g, vitamin B1 trace, agar 15.0g, pH 6.0. Sterilize at 126°C for 30 minutes, cool to get ready;

[0043] Potato extract: Take 200g of peeled potatoes, cut into small pieces, add 1.0L of water and boil for 30min, filter out the potato pieces, and make up the filtrate to 1.0L.

[0044] Liquid fermentation culture: Inoculate 1% of the above-mentioned strain 1 on the above-mentioned liquid med...

Embodiment 2

[0050] 1. Liquid fermentation culture

[0051] Liquid medium: potato extract 1.0L, glucose 20.0g, KH 2 PO 4 3.0g, MgSO 4 .7H 2 O 1.5g, vitamin B1 trace, agar 15.0g, pH 6.0. Sterilize at 126°C for 30 minutes, cool to get ready;

[0052] Potato extract: Take 200g of peeled potatoes, cut into small pieces, add 1.0L of water and boil for 30min, filter out the potato pieces, and make up the filtrate to 1.0L.

[0053] Liquid fermentation culture: Inoculate 1% of the above-mentioned strain 2 on the above-mentioned liquid medium, and ferment at 18°C ​​for 12 days to obtain Tremella liquid strain;

[0054] 2. Solid-state fermentation culture

[0055] Take 200g of Ophiopogon japonicus and 800g of Dendrobium officinale, crush them into 20 meshes, add 2 times the quality of water, after sterilization, inoculate 10% of Tremella fungus liquid strains, ferment for 16 days at 18°C, and then inactivate at high temperature;

[0056] 3. Extraction and preparation

[0057] Add 8 times the...

Embodiment 3

[0059] 1. Liquid fermentation culture

[0060] Liquid medium: potato extract 1.0L, glucose 20.0g, KH 2 PO 4 3.0g, MgSO 4 .7H 2 O 1.5g, vitamin B1 trace, agar 15.0g, pH 6.0. Sterilize at 126°C for 30 minutes, cool to get ready;

[0061] Potato extract: Take 200g of peeled potatoes, cut into small pieces, add 1.0L of water and boil for 30min, filter out the potato pieces, and make up the filtrate to 1.0L.

[0062] Liquid fermentation culture: Inoculate 1% of the above-mentioned strain 2 on the above-mentioned liquid medium, and ferment at 35° C. for 4 days to obtain Tremella liquid strain;

[0063] 2. Solid-state fermentation culture

[0064] Take 900g of Ophiopogon japonicus and 100g of Dendrobium officinale, crush them into 60 meshes, add 2.5 times the quality of water, after sterilization, inoculate 2% of Tremella fungus liquid strains, ferment for 3 days at 35°C, and then inactivate at high temperature;

[0065] 3. Extraction and preparation

[0066] Add 20 times the...

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Abstract

The invention discloses a plant extract and a preparation method and application thereof. The preparation method of the plant extract is characterized by comprising the following steps of (1) using tremella fuciformis for microbial conversion of radix ophiopogonis and dendrobium officinale to obtain a microbial conversion substance; (2) using an alcohol water solution or water for extracting the microbial conversion substance obtained in step (1) to obtain a plant extract. The preparation method is simple and convenient in operation procedure and reasonable in process, and the obtained plant extract has the effects of obviously delaying skin aging and improving the body functions and can promote production of ATP in fibroblasts and inhibit gene expression of metalloproteinase-1 and metalloproteinase-3 in aging fibroblasts. Medicine can be taken orally or applied to the skin, the effects of improving the body functions and resisting aging of the skin and other organs are achieved, and considerable economic and social benefits are obtained.

Description

technical field [0001] The invention relates to a plant extract and its preparation method and application. Background technique [0002] Aging (ageing) refers to the gradual degenerative changes in the tissue structure of the organism with the increase of age after the organism matures under normal conditions. Lowering, a natural phenomenon that tends toward death. [0003] In the long history of human wisdom, human beings have been trying to fight against aging. Human civilization has discovered that the internal and external environment is an important factor affecting aging, such as: ultraviolet radiation, the invasion of toxic substances, etc. are important factors leading to aging. By avoiding the damage of harmful factors and using natural resources to stimulate or improve the body's resistance, aging can be effectively delayed. Human longevity continues to increase as humans battle these factors that contribute to aging. [0004] However, with the development of h...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61Q19/08A61P17/18
CPCA61K8/97A61K36/07A61K36/8968A61K36/8984A61K2236/19A61K2800/85A61Q19/08A61K2300/00
Inventor 翟春涛许华金贺改英张太亮
Owner SHANGHAI SHENG WEI BIOTECHNOLOGY CO LTD