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A kind of Aureobasidium pullulans strain and application thereof

A technology of Aureobasidium pullulans and strains, applied in the direction of fungi, microorganisms, microorganisms, etc., can solve the problems of clear research on liamocin biosynthesis mechanism, complex liamocin biosynthesis pathway, and low yield of liamocin, and simplify the post-processing process and method The effect of simple and easy to implement and simple extraction method

Active Publication Date: 2021-02-26
优鲜工坊(浙江)食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biosynthetic pathway of liamocin is very complicated, and the biosynthetic mechanism of liamocin has not been studied clearly
Scholars represented by Kurosawa T and Price NPJ have done in-depth research on the structure, performance and application of liamocin, but there are few studies on the fermentation of liamocin, and the yield of liamocin in the current prior art is generally not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The method that Aureobasidium pullulans strain of the present invention is used for fermentation to produce liamocin is as follows:

[0046] S1. Activation of strains: transfer the slant bacteria to PDA slant medium, and cultivate them in a constant temperature incubator at 26°C for 2 days;

[0047]S2. Seed cultivation: select 3 loops of well-cultured slant strains and inoculate them in 50 mL of seed culture medium, and cultivate them in a shaker at 26°C and 160r / min for 2 days;

[0048] Among them, the composition of the seed medium is: 5% glucose, 0.2% yeast extract, 0.5% KH 2 PO 4 , 0.04% MgSO 4 ·7H 2 O, 0.04% MnSO 4 ·H 2 O and 0.1% NaCl.

[0049] S3, fermentation culture: divided into two stages

[0050] (1) Inoculate the seed liquid cultivated in step S2 into the fermentation medium (1) at a ratio of 6%, and cultivate it in a shaker at 26° C. and 160 r / min for 3 days;

[0051] Wherein, the composition of fermentation medium (1) is: 5% glucose, 0.2% yeast ex...

Embodiment 2

[0056] The method that Aureobasidium pullulans strain of the present invention is used for fermentation to produce liamocin is as follows:

[0057] S1. Strain activation: transfer the slant bacteria to the PDA slant medium, and cultivate them in a constant temperature incubator at 28°C for 2.5 days;

[0058] S2. Seed culture: select the well-cultured slant strains in step S1, pick 2 loops and inoculate them in 50 mL of seed culture medium, and cultivate them in a shaker at 26° C. and 160 r / min for 2 days;

[0059] Among them, the composition of the seed medium is: 5% glucose, 0.2% yeast extract, 0.5% KH 2 PO 4 , 0.04% MgSO 4 ·7H 2 O, 0.04% MnSO 4 ·H 2 O and 0.1% NaCl.

[0060] S3, fermentation culture: divided into two stages:

[0061] (1) Inoculate the seed solution cultivated in step S2 into the fermentation medium (1) at a ratio of 10%, and cultivate it in a shaker at 28° C. and 200 r / min for 3 days;

[0062] Among them, the composition of fermentation medium (1) is...

Embodiment 3

[0067] The method that Aureobasidium pullulans strain of the present invention is used for fermentation to produce liamocin is as follows:

[0068] S1. Activation of strains: transfer the slant bacteria to PDA slant medium, and cultivate them in a constant temperature incubator at 26°C for 2 days;

[0069] S2. Seed culture: select the slant bacteria cultured well in step S1, pick 3 loops and inoculate them in the seed culture medium, and cultivate them in a shaker at 28°C and 200r / min for 2 days;

[0070] Among them, the composition of the seed medium is: 5% glucose, 0.2% yeast extract, 0.5% KH 2 PO 4 , 0.04% MgSO 4 ·7H 2 O, 0.04% MnSO 4 ·H 2 O and 0.1% NaCl.

[0071] S3, fermentation culture: divided into two stages

[0072] (1) Inoculate the seed solution cultivated in step S2 into the fermentation medium (1) at a ratio of 8%, and cultivate it in a shaker at 28° C. and 180 r / min for 3 days;

[0073] Among them, the composition of the fermentation medium (1) is: 7% ma...

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PUM

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Abstract

The invention relates to an Aureobasidium pullulans strain and an application thereof, belonging to the fields of fermentation engineering and biotechnology. The preservation name of this Aureobasidium pullulans strain is: Aureobasidium pullulans (Aureobasidium pullulans) ZK-502, preservation unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms, preservation address: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, date of deposit: August 20, 2018, deposit number: CGMCC No.16326. The Aureobasidium pullulans strain provided by the invention can efficiently ferment and produce liamocin, and the Aureobasidium pullulans strain does not produce melanin, and the fermentation liquid is light pink to light yellow, which is beneficial to simplify the post-treatment process. The method provided by the present invention is simple and easy, the raw material components used are simple, the output of liamocin can reach 8-10g / L, and it is expected to realize the commercialization and large-scale production of liamocin; the obtained liamocin product is soluble in water and can be used in medicine and cosmetics , Food has broad application prospects.

Description

technical field [0001] The invention relates to an Aureobasidium pullulans strain and an application thereof, belonging to the fields of fermentation engineering and biotechnology. Background technique [0002] Liamocin is one of the new active products of Aureobasidium pullulans. Because its density is heavier than water, it will settle at the bottom when it is still, so it is also called heavy oil. Liamocin currently generally refers to a class of polyol ester compounds that are heavier than water produced by Aureobasidium pullulans. The head group of the polyol ester is mostly mannitol or arabic alcohol, and the tail is a triplet of 3,5-dihydroxydecanoic acid. body or quadruple. [0003] Liamocin has a coconut aroma and is denser than water. The color of the oil varies from light yellow to malachite, and most of the oil is fluorescent. It is slightly soluble in water and easily soluble in organic solvents. It has been proved that the polyol ester has various biological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/62C12R1/645
CPCC12P7/62C12N1/145C12R2001/645
Inventor 魏培莲周丹凤吕磊磊刘士旺肖功年龚金炎柳永鲍文娜袁海娜
Owner 优鲜工坊(浙江)食品有限公司