Brucella biological terrorism warfare agent field gene detection method

A Brucella and gene detection technology, applied in the field of molecular biology, can solve the problems of high detection cost, complicated operation, and difficult operation, and achieve reliable detection, good stability and specificity

Inactive Publication Date: 2019-01-04
CHINESE PEOPLES ARMED POLICE FORCE ACAD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in my country, there is no biological detection method established for the fire brigade to deal with bioterrorism incidents. The inst

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  • Brucella biological terrorism warfare agent field gene detection method
  • Brucella biological terrorism warfare agent field gene detection method
  • Brucella biological terrorism warfare agent field gene detection method

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Embodiment Construction

[0049] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts fall within the protection scope of the present invention.

[0050] The invention provides a kind of brucella bioterrorism warfare agent on-the-spot gene detection method, wherein, comprise the following steps:

[0051] S1. Collect samples from the attack site and process them.

[0052] According to different types of samples collected, different types of processing are required to obtain samples that can be used for detection. When the sample collected is a swab sample, the sample processing steps are:

[0053] 1) Insert the cotton swa...

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Abstract

The invention discloses a brucella biological terrorism warfare agent field gene detection method. The brucella biological terrorism warfare agent field gene detection method comprises the steps of S1, collecting an attack site sample and treating the attack site sample; S2, using a brucella field detection kit to carry out real-time fluorescent quantitative PCR inspection on the treated sample, wherein the brucella field detection kit comprises negative control, positive control and brucella specific upstream and downstream primers; correspondingly using the treated sample, the negative control and the positive control as substrates to carry out same real-time fluorescent quantitative PCR treatment under a same condition; and analyzing a detection result of a sample to be detected so as to judge whether the treated sample carries brucella. The brucella biological terrorism warfare agent field gene detection method disclosed by the invention can directly use the sample as the PCR detection primer, and is suitable for rapidly screening samples with brucella genome DNA from environment samples with various phase states. A solution is provided for fire fighting forces to rapidly recognize biological hazards of biological terrorism attack fields.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for on-site gene detection of Brucella bioterrorism warfare agents. Background technique [0002] Brucella can infect humans and livestock and cause brucellosis. In my country, brucellosis is a Class B infectious disease. The World Health Organization defines it as a Class B infectious disease. It is highly contagious and harmful to humans. After recurrent brucellosis becomes chronic, it will leave sequelae leading to permanent disability, and will be transmitted to the next generation. So far, more than 170 countries and regions in the world have reported outbreaks. Despite my country's mandatory intervention, the brucellosis epidemic is still growing at the current double-digit percentage rate, which poses a serious threat to my country's economic construction, people's health, and national security. [0003] With the continuous expansion of the emergency man...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6851C12Q1/06C12R1/01
CPCC12Q1/6851C12Q1/689C12Q2531/113C12Q2545/113C12Q2563/107
Inventor 王慧飞宗鹏康青春
Owner CHINESE PEOPLES ARMED POLICE FORCE ACAD
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