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QTLs (quantitative trait loci) of related traits of radish fleshy root and their positioning method

A positioning method, a technique for succulent roots

Active Publication Date: 2019-01-04
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0005] In order to solve the defect that QTLs related to radish fleshy root length have not been studied in the prior art, and the previous radish linkage map has no connection with the physical map of radish genome, the present invention provides QTLs for radish fleshy root-related traits and its positioning method , the purpose of realization is that the present invention combines the linkage map of radish with its genome physical Map integration, mapping to the QTLs controlling radish fleshy root traits, including root length, root diameter and root weight, thereby improving the efficiency of variety breeding

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  • QTLs (quantitative trait loci) of related traits of radish fleshy root and their positioning method
  • QTLs (quantitative trait loci) of related traits of radish fleshy root and their positioning method
  • QTLs (quantitative trait loci) of related traits of radish fleshy root and their positioning method

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Embodiment 1

[0039] Example 1: The present invention provides a method for locating QTLs of radish fleshy root-related traits. The method includes the following steps: (1) Using two high-generation radish inbred lines'pp12q-4-2' and '36- 2'as parent material for offspring F 1 , F 2 Separation population construction: the female parent'pp12Q-4-2' is a cultivated long-pod radish with slender roots and does not expand; the male parent '36-2' is a cultivated radish with a long cylindrical fleshy root with white skin and white flesh . The phenotypes of fleshy roots of the two parent materials are significantly different. F 1 Obtained by artificial cross-pollination of two parental materials. 1 Strict artificial bud stage self-pollination to obtain F 2 . Parents (30 plants each), F 1 (30 strains) and F 2 (330 plants) were sown in the greenhouse of the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences in the fall of 2013, and were cultivated and managed by conven...

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Abstract

The invention discloses 13 QTLs (quantitative trait loci) of related traits of radish fleshy root, which locate at chromosomes Chr1, Chr2, Chr3, Chr4, Chr5, Chr6, Chr7 and Chr8 respectively and have gene sequences shown as SEQ ID NO. 1 to SEQ ID NO. 48, and also discloses a method of positioning the above QTLs. The method includes: (1) constructing F2 segregation population; (2) acquiring and statistically analyzing related phenotypic trait data (root length, root thickness and root weight) of fleshy root of female and male parents and F1 and F2 segregation populations; (3) extracting DNA forthe female and male parents and F1 and F2 segregation populations; (4) screening polymorphic SSR (simple sequence repeat) primers, and genotyping the female and male parents and F1 and F2 segregationpopulations; (5) constructing a genetic map; (6) using the software MapQTL 4.0 to perform QTLs analysis. The QTLs and the method have the advantages that linkage map of radishes is connected to its genome physical map for the first time, 2 QTLs to control the radish root length are positioned for the first time, and variety breeding efficiency is improved accordingly.

Description

Technical field [0001] The present invention belongs to the technical field of molecular biology, and specifically relates to QTLs of radish fleshy root-related traits and a positioning method thereof. Background technique [0002] Radish (Raphanus sativus L., 2n=2×=18) is an important root vegetable in the world. Its fleshy root contains a variety of ingredients beneficial to human health. The development of fleshy roots is crucial for determining the yield and quality of radish product organs. Similar to other root plants, the development of radish fleshy roots is also regulated by many factors such as genetic, environmental and physiological factors. At present, attention has been paid to the genetic and molecular mechanism of fleshy root development in radish. [0003] The field trait investigation of fleshy roots is time-consuming and laborious, which limits the genetic improvement of fleshy roots of radish. The development of molecular markers based on DNA sequences such ...

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李锡香孙玉燕邱杨汪精磊王海平张晓辉宋江萍段蒙蒙
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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