A method for inducing embryogenic callus from immature embryos of Iris germanica

A technology of embryogenic callus and callus, which is applied in the field of embryogenic callus induced by German iris immature embryos, can solve the problems of difficult breeding, long breeding cycle, and few hybrid offspring seedlings, and achieve the induction rate High, easy to operate, and the effect of improving the induction rate

Active Publication Date: 2021-10-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the breeding method of German iris is mainly conventional hybrid breeding, which has a long breeding cycle, fewer hybrid offspring seedlings, and difficult breeding. However, embryogenic callus induced by immature embryos is used to construct regenerated plants, which can proliferate a large number in a short period of time. Hybrid offspring provide convenience for the selection and breeding of fine varieties

Method used

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  • A method for inducing embryogenic callus from immature embryos of Iris germanica
  • A method for inducing embryogenic callus from immature embryos of Iris germanica
  • A method for inducing embryogenic callus from immature embryos of Iris germanica

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Experimental program
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Embodiment 1

[0038] In the present invention, MS medium was purchased from Nanjing Tengchun Biological Co., Ltd., with a specification of 50 L; agar powder was purchased from Nanjing Qingke Biological Co., Ltd., with a specification of 500 g; 2,4-D (2,4-Dichlorophenoxyacetic acid, 2, 4-dichlorophenoxyacetic acid) was purchased from Nanjing Tengchun Biological Co., Ltd., and the specification was 10 g, which was packaged by Sigma; 6-BA (6-Benzylaminopurine, 6-benzylaminoadenine) was purchased from Nanjing Tengchun Biological Co., Ltd. company, the specification is 5g, and the product is repackaged by Sigma Company.

[0039] The steps are as follows:

[0040] (1) Material preparation:

[0041] 45 days after selfing in Iris germanica ‘00258’, take well-grown capsules and store them in a refrigerator at 4°C for later use;

[0042] (2) explant disinfection:

[0043] Soak the capsules in step (1) in a 10% detergent solution by volume for 10 minutes, then rinse them under running water for 30 ...

Embodiment 2

[0053] Take well-grown stem tip, young tender ovary, capsule immature embryo, young tender flower bud, root tip respectively as explants, and all the other processing steps are the same as in Example 1, and the pollution rate, The callus induction rate, the state and color of the callus and the time when the callus was induced were recorded. The results are shown in Table 1.

[0054] Table 1 Effects of different explants on callus induction of Iris germanica

[0055]

[0056]

[0057] Analysis of the above data shows that the present invention uses German Iris immature embryos as explants, which has the characteristics of low pollution rate, short callus induction time and easy access to materials. Its outstanding advantage is that the callus induction rate is as high as 95%. %, the operation is simple, and the obtained callus has good embryogenicity.

Embodiment 3

[0059] Adopt the processing method among the embodiment 1, its difference is the culture condition of immature embryo in the step (3), count embryogenic callus induction rate, callus tissue diameter and obtain callus color respectively after inoculation 30d, the result is as table 2 shown.

[0060] Table 2 Effects of different temperatures and photoperiods on callus induction rate

[0061]

[0062] From the analysis of the above data, it can be seen that the present invention adopts 25° C. and dark conditions as the culture conditions of immature embryos, which can make the callus induction rate the highest, up to 93%.

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Abstract

The invention belongs to the technical field of cell engineering tissue culture, and in particular relates to a method for inducing embryogenic callus from german iris immature embryos. The specific steps include: material preparation, explant disinfection, explant inoculation, induction of embryogenic callus and proliferation of embryogenic callus. The invention uses german iris immature embryos as explants, has low pollution rate, high callus induction rate, good embryogenicity of callus, and is easy to operate.

Description

technical field [0001] The invention relates to the technical field of cell engineering tissue culture, in particular to a method for inducing embryogenic callus from german iris immature embryos. Background technique [0002] German iris (Iris germanica L.) is a perennial herb of the genus Iris (Iris.L) of the family Iridaceae (Iridaceae), and is one of the world-renowned perennial flowers. High ornamental value, widely cultivated all over the world, has huge development space in landscaping, potted plants and ground cover applications. [0003] At present, the German iris varieties used in China are all imported from abroad, not only the number of varieties is small, the provenance is limited, but also the price is expensive. Urgently hope to obtain more german iris seedlings with excellent traits to meet various demands. Due to the low natural seed setting rate of German iris, it is difficult to use seeds for propagation, and the introduction of mother plants is limited...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张永侠原海燕杨永恒刘清泉王银杰顾春笋徐晓洋张婷孙玉明黄苏珍
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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