Culture medium capable of amplifying number of endothelial progenitor cells

A technology of endothelial progenitor cells and culture medium, which is applied in the field of culture medium for expanding the number of endothelial progenitor cells, which can solve the problems of impaired function and low number of endothelial progenitor cells, reduce pollution, facilitate separation, and have good safety Effect

Inactive Publication Date: 2019-01-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, compared with normal people, the number of endothelial progenitor

Method used

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  • Culture medium capable of amplifying number of endothelial progenitor cells
  • Culture medium capable of amplifying number of endothelial progenitor cells

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example one:

[0015] In this example, using EGM-2 as the basic medium, 10% fetal bovine serum, 5g / L soybean phytohydrolyzed protein, 10μg / L VEGF, 2μg / L bFGF, 20μg / L epidermal growth factor , 50μM vitamin C, 1μM AR-A014418.

[0016] This embodiment reduces the amount of fetal bovine serum used in conventional culture media, and uses soybean plant hydrolyzed protein, which has a lower cost than conventional culture media, and solves the problem of premature cell death and morphological unsaturation caused by reducing the use of fetal bovine serum. The problem of using AR-A014418 to amplify the number of endothelial progenitor cells.

Example Embodiment

[0017] Embodiment two:

[0018] In this example, under the condition of using EGM-2 as the basal medium, 10% fetal bovine serum was used, and then 10g / L of soybean plant hydrolyzed protein, 50μg / L of VEGF, 10μg / L of bFGF, 100μg were added to the medium / L epidermal growth factor, 200μM vitamin C, 5μM AR-A014418.

[0019] This embodiment reduces the amount of fetal bovine serum used in conventional culture media, and uses soybean plant hydrolyzed protein, which has a lower cost than conventional culture media, and solves the problem of premature cell death and morphological unsaturation caused by reducing the use of fetal bovine serum. The problem of using AR-A014418 to amplify the number of endothelial progenitor cells.

Example Embodiment

[0020] Embodiment three:

[0021] In this example, under the condition of using EGM-2 as the basal medium, 10% fetal bovine serum was used, and 7g / L soybean phytohydrolyzed protein, 30μg / L VEGF, 7μg / L bFGF, 70μg were added to the medium. / L epidermal growth factor, 100μM vitamin C, 3μM AR-A014418.

[0022] This embodiment reduces the amount of fetal bovine serum used in conventional culture media, and uses soybean plant hydrolyzed protein, which has a lower cost than conventional culture media, and solves the problem of premature cell death and morphological unsaturation caused by reducing the use of fetal bovine serum. The problem of using AR-A014418 to amplify the number of endothelial progenitor cells.

[0023] The low-serum culture medium developed by the present invention is similar in shape to conventionally cultured 20% fetal bovine serum, reduces pollution, facilitates the separation of downstream products of cells, and has good safety; compared with commercial culture mediu...

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Abstract

The invention discloses a culture medium capable of amplifying the number of endothelial progenitor cells. Under the condition that EGM-2 is adopted as a basic culture medium, and on the basis of conventional 20% fetal calf serum, the serum concentration can be reduced to 10%, and 5-10g/L soybean plant protein protolysate, 10-50mu g/L VEGF (Vascular Endothelial Growth Factor), 2-10mu g/L bFGF, 20-100mu g/L epidermal growth factors, 50-200mu M of vitamin C and 1-5mu M of AR-A014418 are put into the culture medium. Compared with the 20% fetal calf serum of a conventional culture medium, the culture medium disclosed by the invention is similar in morphology, is capable of reducing pollution, is beneficial to separation of downstream products of cells, and is good in security; compared with acommercial culture medium, the culture medium is reduced in cost and is applicable to on-scale production of endothelial progenitor cells; by adopting the culture medium disclosed by the invention, anAR-A014418 component is increased, and as GSKi, the AR-A014418 is capable of remarkably increasing the number of endothelial progenitor cells (EPCs).

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a culture medium capable of expanding the number of endothelial progenitor cells, which is used for the cultivation and proliferation of endothelial progenitor cells. Background technique [0002] Coronary artery disease is the most common disease among cardiovascular diseases, and it is also a disease with high mortality and morbidity. The main reason is the stenosis of blood vessels caused by atherosclerosis. The most common way to treat coronary artery disease is to use a balloon to open narrowed blood vessels and then permanently place a metal support material, known as a stent. The role of bare metal stents is only to keep blood vessels open; drug-eluting stents not only keep blood vessels open, but also slowly release the drug coating to help prevent restenosis or re-blockage of blood vessels after surgery. However, restenosis after coronary stent implantation and lon...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/069C12N2500/38C12N2501/11C12N2501/115C12N2501/165C12N2501/727C12N2501/998
Inventor 张基昌张明刘永利石旭东刘斌郭子源赵卓刘一航吴熙张建奇
Owner JILIN UNIV
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