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Culture medium capable of amplifying number of endothelial progenitor cells

A technology of endothelial progenitor cells and culture medium, which is applied in the field of culture medium for expanding the number of endothelial progenitor cells, which can solve the problems of impaired function and low number of endothelial progenitor cells, reduce pollution, facilitate separation, and have good safety Effect

Inactive Publication Date: 2019-01-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, compared with normal people, the number of endothelial progenitor cells in patients with coronary heart disease is not only small but also impaired

Method used

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  • Culture medium capable of amplifying number of endothelial progenitor cells
  • Culture medium capable of amplifying number of endothelial progenitor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In this example, under the condition that EGM-2 is used as the basal medium, 10% fetal bovine serum, 5 g / L soybean plant hydrolyzed protein, 10 μg / L VEGF, 2 μg / L bFGF, and 20 μg / L epidermal growth factor are used , 50 μM Vitamin C, 1 μM AR-A014418.

[0016] This embodiment reduces the amount of fetal bovine serum used in conventional medium, uses soybean plant hydrolyzed protein, and its cost is lower than conventional medium, and solves the problem of premature cell death and unsaturated morphology caused by reducing the use of fetal bovine serum question, the number of endothelial progenitor cells was expanded using AR-A014418.

Embodiment 2

[0018] In this example, under the condition that EGM-2 is used as the basal medium, 10% fetal bovine serum is used, and 10 g / L soybean plant hydrolyzed protein, 50 μg / L VEGF, 10 μg / L bFGF, and 100 μg are added to the medium. / L of epidermal growth factor, 200 μM of vitamin C, 5 μM of AR-A014418.

[0019] This embodiment reduces the amount of fetal bovine serum used in conventional medium, uses soybean plant hydrolyzed protein, and its cost is lower than conventional medium, and solves the problem of premature cell death and unsaturated morphology caused by reducing the use of fetal bovine serum Question, using AR-A014418 to expand endothelial progenitor cell numbers.

Embodiment 3

[0021] In this example, under the condition that EGM-2 is used as the basal medium, 10% fetal bovine serum is used, and 7 g / L soybean plant hydrolyzed protein, 30 μg / L VEGF, 7 μg / L bFGF, 70 μg / L of epidermal growth factor, 100 μM of vitamin C, 3 μM of AR-A014418.

[0022] This embodiment reduces the amount of fetal bovine serum used in conventional medium, uses soybean plant hydrolyzed protein, and its cost is lower than conventional medium, and solves the problem of premature cell death and unsaturated morphology caused by reducing the use of fetal bovine serum Question, using AR-A014418 to expand endothelial progenitor cell numbers.

[0023] The low-serum culture medium developed by the present invention is similar in shape to conventionally cultured 20% fetal bovine serum, reduces pollution, is beneficial to the separation of downstream products of cells, and has good safety; compared with commercial culture medium, the cost is reduced, Suitable for bulk production of end...

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Abstract

The invention discloses a culture medium capable of amplifying the number of endothelial progenitor cells. Under the condition that EGM-2 is adopted as a basic culture medium, and on the basis of conventional 20% fetal calf serum, the serum concentration can be reduced to 10%, and 5-10g / L soybean plant protein protolysate, 10-50mu g / L VEGF (Vascular Endothelial Growth Factor), 2-10mu g / L bFGF, 20-100mu g / L epidermal growth factors, 50-200mu M of vitamin C and 1-5mu M of AR-A014418 are put into the culture medium. Compared with the 20% fetal calf serum of a conventional culture medium, the culture medium disclosed by the invention is similar in morphology, is capable of reducing pollution, is beneficial to separation of downstream products of cells, and is good in security; compared with acommercial culture medium, the culture medium is reduced in cost and is applicable to on-scale production of endothelial progenitor cells; by adopting the culture medium disclosed by the invention, anAR-A014418 component is increased, and as GSKi, the AR-A014418 is capable of remarkably increasing the number of endothelial progenitor cells (EPCs).

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a culture medium capable of expanding the number of endothelial progenitor cells, which is used for the cultivation and proliferation of endothelial progenitor cells. Background technique [0002] Coronary artery disease is the most common disease among cardiovascular diseases, and it is also a disease with high mortality and morbidity. The main reason is the stenosis of blood vessels caused by atherosclerosis. The most common way to treat coronary artery disease is to use a balloon to open narrowed blood vessels and then permanently place a metal support material, known as a stent. The role of bare metal stents is only to keep blood vessels open; drug-eluting stents not only keep blood vessels open, but also slowly release the drug coating to help prevent restenosis or re-blockage of blood vessels after surgery. However, restenosis after coronary stent implantation and lon...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/069C12N2500/38C12N2501/11C12N2501/115C12N2501/165C12N2501/727C12N2501/998
Inventor 张基昌张明刘永利石旭东刘斌郭子源赵卓刘一航吴熙张建奇
Owner JILIN UNIV
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