Pretreatment method of lignocellulosic biomass matched with saccharification of cellulosome whole bacteria
A cellulosome and wood fiber technology, which can be used in biofuels, fermentation, etc., can solve the problems such as the applicability of whole cellulosome saccharification that has not been investigated
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Embodiment 1
[0030] 1) Straw crushing
[0031] Extrude and crush the wheat straw with a twin-screw extruder to a particle size of 0.05-1.0 cm to obtain a pretreated material A, which is collected in a ziplock bag for use.
[0032] 2) Sulfonation pretreatment
[0033] Weigh 80g of the dry mass of the pretreated material A prepared in step 1), put it into the reactor group tank (1.5 liters of single tank volume), and weigh 16g of the ammonium sulfite solid (equivalent to the dry mass of the pretreated material A) at the same time. 20% by weight) was added into water and stirred until completely dissolved, and the dissolved medicinal solution was poured into the tanks of the reaction kettle group, so that the liquid-solid ratio of the whole reaction system was 6:1, the lid was tightened, and the rotary reaction kettle was loaded into Medium, heat to 160 degrees, keep warm for 1 hour. After the reaction, lower the temperature and cool down, open the kettle to discharge the material, squeeze ...
Embodiment 2
[0041] 1) Straw crushing
[0042] Step is the same as Step 1 in Example 1.
[0043] 2) Sulfonation pretreatment
[0044] Step is the same as step 2 in embodiment 1.
[0045] 3) Xylan hydrolysis pretreatment
[0046] Weigh 1g of pretreated solid material B dry mass in step 2) and add it to a 50ml glass bottle, and add a pre-prepared citric acid / sodium citrate buffer solution with pH=4.5-5.0 into the bottle to make the reaction system pretreated The absolute dry content of solid material B is 5%, then add 0.005g xylanase (amount equivalent to 0.005g / g substrate, label enzyme activity 13200U / g) to it, place the bottle at constant temperature after the bottle is tightly closed In the air shaker, the temperature was kept at 50 degrees, and the rotation speed was 120 rpm. After the hydrolysis reaction was performed for 24 hours, the solid-liquid separation was carried out. The liquid is mainly xylose and xylooligosaccharides degraded by hemicellulose; the solid is mainly cellulo...
Embodiment 3
[0053] 1) Corn stalk crushing
[0054] Extrude and crush the wheat straw with a twin-screw extruder to a particle size of 0.5-2.0 cm to obtain a pretreated material A, which is collected in a ziplock bag for use.
[0055] 2) Sulfonation pretreatment
[0056] Weigh 70g of pretreatment material A absolute dry mass prepared in step 1), put it into the reactor group tank (1.5 liters of single tank volume), and weigh 10.5g of ammonium sulfite solid (equivalent to pretreatment material A absolute 15% of the dry weight) was added into water and stirred until completely dissolved, and the dissolved medicinal liquid was poured into the tanks of the reaction kettles, so that the liquid-solid ratio of the entire reaction system was 5:1, the lid was tightened, and the rotary reaction In the kettle, heat up to 150°C at a rate of 2°C per minute, and keep the temperature for 1.5 hours. After the reaction, cool down, open the kettle and discharge the material, squeeze the cooked material to...
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