Preparation method of fish collagen oligopeptide

A technology of fish collagen and oligopeptide, applied in the direction of fermentation, can solve the problems of difficult absorption and utilization, high content of heavy metals, unable to meet food-grade requirements, etc., and achieve the effect of improving quality and reducing waste.

Inactive Publication Date: 2019-01-11
新昌县赛因斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The prior art, such as the Chinese invention patent with the authorized announcement number CN 102286589 B, discloses a method for extracting soft-shelled turtle oligopeptides. After the soft-shelled turtle is used as a raw material and enzymolyzed with alkaline protease, the flavor protease is used to exocut and adjust the flavor. Ultrafiltration of the enzymolysis solution, the addition of alkaline protease is 0.2-5% of the weight of the raw soft-shelled turtle, the addition of flavor protease is 0.1-2% of the weight of the raw soft-shelled turtle, and the ultr...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of fish collagen oligopeptide comprises the following production processes:

[0032] Raw material cleaning → gel extraction → enzymatic hydrolysis → metal removal → decolorization, deodorization and desalination → membrane filtration → nanofiltration → concentration → sterilization filtration → spray drying.

[0033] The preparation method of fish collagen oligopeptide specifically comprises the following steps:

[0034] (1) Cleaning: Wash the fish skin with food grade 0.1% sodium hydroxide and table salt for 2 hours according to the ratio of material to liquid 1:3, drain the water, and then wash it with tap water for 5 times until the water is translucent and colorless , pH is 8, standby;

[0035] (2) Gel extraction: After feeding, add high-temperature water according to the production instructions, adjust the pH to 6.0, and extract the gel at 70°C;

[0036] (3) Enzymolysis: adjust pH to 8.0, temperature 50°C, use 0.02% fish skin compound flavo...

Embodiment 2

[0046] The preparation method of fish collagen oligopeptide comprises the following production processes:

[0047] Raw material cleaning → gel extraction → enzymatic hydrolysis → metal removal → decolorization, deodorization and desalination → membrane filtration → nanofiltration → concentration → sterilization filtration → spray drying.

[0048] The preparation method of fish collagen oligopeptide specifically comprises the following steps:

[0049] (1) Cleaning: Wash the fish skin with food grade 0.2% sodium hydroxide and table salt for 3 hours according to the ratio of material to liquid 1:3, drain the water, and then wash it with tap water for 6 times until the water is translucent and colorless , pH is 8, standby;

[0050] (2) Gum extraction: After feeding, add high-temperature water according to the production instructions, adjust the pH to 6.2, and extract the gel at 72°C;

[0051] (3) Enzymolysis: adjust the pH to 8.3, the temperature is 52°C, use 0.05% fish skin com...

Embodiment 3

[0061] The preparation method of fish collagen oligopeptide comprises the following production processes:

[0062] Raw material cleaning → gel extraction → enzymatic hydrolysis → metal removal → decolorization, deodorization and desalination → membrane filtration → nanofiltration → concentration → sterilization filtration → spray drying.

[0063] The preparation method of fish collagen oligopeptide specifically comprises the following steps:

[0064] (1) Cleaning: Wash the fish skin with food grade 0.3% sodium hydroxide and table salt for 4 hours according to the ratio of material to liquid ratio of 1:4, drain the water, and then wash it with tap water for 8 times until the water is translucent and colorless , pH is 9, standby;

[0065] (2) Gum extraction: After feeding, add high-temperature water according to the production instructions, adjust the pH to 6.5, and extract the gel at 75°C;

[0066] (3) Enzymolysis: adjust pH to 8.5, temperature 55°C, use 1.0% fish skin compou...

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Abstract

The invention discloses a preparation method of fish collagen oligopeptide, which takes marine fish skin as raw material, and is subjected to enzymolysis and other treatments to prepare a product witholigopeptide content higher than 96%, wherein the protease used in the enzymatic hydrolysis process contains L-Sodium malate and trimethylglycine can promote the hydrolysis of collagen and increase the yield of small molecular fish collagen oligopeptide. The fish collagen oligopeptide can be widely used in functional food and pharmaceutical industries such as wine industry, beverage industry, condiment industry, health care industry, oral liquid, tablet, capsule and so on. The fish collagen oligopeptide prepared by the invention has the beneficial effects that the molecular weight of the fishcollagen oligopeptide is concentrated in 300-1000Da small molecule mixed peptides, easy to be absorbed by small intestine, human skin and other direct, reduce the waste of nutrients; and the contentof heavy metals is small, the quality is better.

Description

technical field [0001] The invention relates to the technical field of preparation of oligopeptides, in particular to a preparation method of fish collagen oligopeptides. Background technique [0002] Collagen is widely found in connective tissues such as animal skin, bone, tendon, and membrane, and is the main component of connective tissue. In the past, the production of collagen mainly used terrestrial animals with abundant resources, such as the skin and bone of cattle and pigs, as raw materials for processing and production. Although fish collagen has long been recognized by humans, such as tortoise shell glue and yellow fish glue, etc., it has not been utilized as an important industrial application material. It is mainly a product that relies on marine fishing, and its source is unstable and lacks scale. In recent years, because terrestrial animals are affected by various factors such as the environment and infectious diseases, the safety of their proteins has been q...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC12P21/06C07K1/14C07K1/34
Inventor 徐康
Owner 新昌县赛因斯生物科技有限公司
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